Femto Signal Western ECL Substrate
Order Information
| Package | Catalog No. | Price(In USD) | Qty (Quantity) | Sum(In USD) |
|---|---|---|---|---|
| 100 ml | R-ECLF01 | 220 | ||
| 200 ml | R-ECLF02 | 395 | ||
| 500 ml | R-ECLF03 | 722 | ||
| Sub total: | ||||
| Shipping Cost: | 169.00 | |||
| Total: | ||||
Introduction to Femto Signal Western ECL Substrate
The Femto Signal Western ECL Substrate is a hypersensitive ECL chemiluminescence kit produced by Genemedi for the detection of horseradish peroxidase (HRP) on immunoblots. It detects low-femtogram (mid-zeptomole) amounts of protein in bands on nitrocellulose or PVDF membranes when probed with appropriate primary and secondary antibodies. It is compatible with different membranes, blocking reagents, and a wide range of antibody dilutions making it an ideal choice for most western blotting applications.
Properties
| Femto Signal Western ECL Substrate | |
|---|---|
| Quantity/Unit | 2 bottles. |
| Form | Liquid. |
| Sipping and Storage Guidelines | Shipped at 4°C. Stable for 2 years at 4 °C and 6 months at room temperature with protection from light. To avoid repeated thawing and freezing, keep the kit at 4 °C for frequent use. |
Advantages
1. Femtogram sensitivity—detect femtogram amounts of target protein on nitrocellulose or PVDF membrane.
2. High signal stability—incubated blots provide stable signal duration over the critical 4 hour time period with up to 24 hours of light output under optimal conditions.
3. Stable reagent—kit components are stable for 1 year at 4°C or 6 months at room temperature.
4. Economical—optimized for dilute antibody concentrations:
· 1ng to 0.2 µg/mL primary antibody (1:5,000 to 1:100,000 dilution from 1µg/mL stock).
· 2 to 10 ng/mL secondary antibody (1:100,000 to 1:500,000 dilution from µg/mL stock).
5. Exceptional robustness—Low abundance target proteins that cannot be detected by conventional ECL substrates can be detected if substrate is used with optimized antibody concentration and block buffer.
Applications and Figures
Technical Documents
1. For more information please refer to 
Femto Signal Western ECL Substrate User Manual
Frequently Asked Questions(FAQs)
- 1. What is the recommended dilution range for Femto Signal Western ECL Substrate?
-
Answer
Primary antibody recommended dilution range is 1 to 0.2 µg/mL primary antibody (1:5,000 to 1:100,000 dilution from 1µg/mL stock). Secondary antibody recommended dilution range is 2 to 10 ng/mL secondary antibody (1:100,000 to 1:500,000 dilution from µg/mL stock).
- 2. What is the sensitivity of Femto Signal Western ECL Substrate?
-
Answer
Femto Signal Western ECL Substrate enables detection of low-femtogram amounts of target protein on nitrocellulose or PVDF membrane.
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