Lentivirus vector system (lentivirus expression system, lentivirus packaging plasmid system) (Amp and Kanamycin)

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Lentivirus vector system - Introduction

GeneMedi's lentivirus Vector System, also named the lentivirus expression system or lentivirus packaging plasmid system, is a powerful tool for in-vitro & in-vivo gene delivery, shRNA mediated RNA interference (RNAi), gene editing and stable cellline development. You can easily produce a recombinant lentivirus particle in HEK293T or HEK293FT cell line in high titer using GeneMedi's lentivirus Vector System.

The Genemedi lentivirus vector system including 3 lentivirus packaging plasmids: the lentivirus expression plasmids with different promoters and tags, an envelope protein VSV-G expressing plasmid pMD2G and a packaging plasmid pSPAX2 expressing Gag-pol and Tat.

GeneMedi offers both Ampicillin (Amp) resistance and Kanamycin (Kan) resistance for the Lentivirus Vector Systems.

Figure 1: Workflow of lentivirus packaging

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GeneMedi has developed a variety of lentivirus expression vectors with different expression cassettes, containing kinds of verified promoters and reporters including GFP, zsgreen, RFP, mcherry and luciferase. The GeneMedi's lentivirus expression vectors have been proved very suitalble for unique gene overexpression or shRNA-mediated knock-down (also called RNAi (RNA interference ). You can also achieve gene knock-out(KO) or gene editing using our Crispr-cas9-gRNA lentivirus expression vector.



Lentivirus vector system (Amp and Kanamycin)


Cat.NO Product Name Organization Type Price(In USD)
P-PK23 Lentivirus vector system (Amp resistance)
1. PMD2G, Cat No.: P-ALV-C01; 5ug
2. pSPAX2, Cat No.: P-ALV-C02; 5ug
3. Lentivirus expression vectors:
pGMLV-CMV-MCS-EF1-ZsGreen (Cat No.: P-ALV-B52), 5ug
(Expression vector refers only to B52, if you need other expression vector, please check the table below)
Academic $2,430.00
P-PK23 Lentivirus vector system (Amp resistance)
1. PMD2G, Cat No.: P-ALV-C01; 5ug
2. pSPAX2, Cat No.: P-ALV-C02; 5ug
3. Lentivirus expression vectors:
pGMLV-CMV-MCS-EF1-ZsGreen (Cat No.: P-ALV-B52), 5ug
(Expression vector refers only to B52, if you need other expression vector, please check the table below)
Industry R&D
(excluding
CRO&CDMO&CXO)
P-PK23 Lentivirus vector system (Amp resistance)
1. PMD2G, Cat No.: P-ALV-C01; 5ug
2. pSPAX2, Cat No.: P-ALV-C02; 5ug
3. Lentivirus expression vectors:
pGMLV-CMV-MCS-EF1-ZsGreen (Cat No.: P-ALV-B52), 5ug
(Expression vector refers only to B52, if you need other expression vector, please check the table below)
CRO&CDMO&
CXO&CMC&
Manufacturing company
P-PK23 Lentivirus vector system (Amp resistance)
1. PMD2G, Cat No.: P-ALV-C01; ≥10mg
2. pSPAX2, Cat No.: P-ALV-C02; ≥10mg
3. Lentivirus expression vectors:
pGMLV-CMV-MCS-EF1-ZsGreen (Cat No.: P-ALV-B52), ≥10mg
(Expression vector refers only to B52, if you need other expression vector, please check the table below)
All
P-PK23-Kan Lentivirus vector system (Kan resistance)
1. PMD2G(Kan resistance), Cat No.: P-ALV-C01-Kan; ≥10mg
2. pSPAX2(Kan resistance), Cat No.: P-ALV-C02-Kan; ≥10mg
3. Lentivirus expression vectors:
pGMLV-CMV-MCS-EF1-ZsGreen(Kan resistance) (Cat No.: P-ALV-B52-Kan), ≥10mg
All
GeneMedi offers both seed and amplification services for the Amp-resistant Lentivirus Vector System. GeneMedi only offers amplification services and do not provide seed for the Kan-resistant Lentivirus Vector System currently.
If you want to obtain higher quantities of plasmids (mg), please visit GM TransExcellent Plasmid DNA Rapid Preparation Service.
Terms of sale:
For Seed (5ug): The purchaser may not reverse engineer this product to extract the sequence for independent use. The purchaser may not transfer this product to others for manufacturing purposes. The purchaser may not use this material to manufacture this product for any other party.

For plasmid production amplification (≥10mg): The purchaser may not reverse engineer this product to extract the sequence for independent use. The purchaser may not transfer this product to others for manufacturing purposes. The purchaser may not use this material to manufacture this product for themselves or any other party.

Note: Industry R&D excludes CRO&CDMO&CXO; Others includes CRO&CDMO&CXO&CMC&Manufacturing company


Helper plasmids of lentivirus packaging (Amp and Kanamycin)


Helper plasmids PMD2G (Amp resistance) pSPAX2 (Amp resistance)
Catalog No. P-ALV-C01 P-ALV-C02
Description an envelope protein VSV-G expressing plasmid lentivirus packaging plasmids expressing Gag-pol and Tat
Map and sequence

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Cat.No Product Name Detail
P-ALV-C01 pMD2G; 5ug (seed) / ≥10mg (amplification services) Details
P-ALV-C01-Kan pMD2G (Kan resistance); ≥10mg (amplification services) Details
P-ALV-C02 pSPAX2; 5ug (seed) / ≥10mg (amplification services) Details
P-ALV-C02-Kan pSPAX2 (Kan resistance); ≥10mg (amplification services) Details

Lentivirus expression vectors (lentivirus expression plasmids) (Amp and Kanamycin)



Note: Industry R&D excludes CRO&CDMO&CXO; Others includes CRO&CDMO&CXO&CMC&Manufacturing company

Cat.No Expression Name of Vectors Promoter Reporter Cre-dependent TAG (unfusion) Order
P-ALV-B01 Overexpression pCDH-MSCV-MCS-EF1-copGFP-T2A-Puromycin MSCV copGFP Puromycin NULL Picture loading failed.
P-ALV-B02 Overexpression pGMLV-CMV-Circ-MCS-EF1-ZsGreen-T2A-Puromycin CMV ZsGreen Puromycin NULL Picture loading failed.
P-ALV-B03 Overexpression pGMLV-CMV-MCS-3flag-EF1-fLUC-T2A-Puromycin CMV Luciferase Puromycin 3FLAG(C-terminal) Picture loading failed.
P-ALV-B04 Overexpression pGMLV-CMV-MCS-EF1-fLUC-T2A-Puromycin CMV Luciferase Puromycin NULL Picture loading failed.
P-ALV-B05 Overexpression pGMLV-CMV-MCS-EF1-mCherry-T2A-Puromycin CMV mCherry Puromycin NULL Picture loading failed.
P-ALV-B06 Overexpression pGMLV-CMV-MCS-3flag-EF1-mCherry-T2A-Puromycin CMV mCherry Puromycin 3FLAG(C-terminal) Picture loading failed.
P-ALV-B07 Overexpression pGMLV-CMV-MCS-EF1-Puromycin CMV Null Puromycin NULL Picture loading failed.
P-ALV-B08 Overexpression pGMLV-CMV-MCS-3flag-EF1-Puromycin CMV Null Puromycin 3FLAG(C-terminal) Picture loading failed.
P-ALV-B09 Overexpression pGMLV-CMV-MCS-3flag-T2A-ZsGreen CMV ZsGreen Null 3FLAG(C-terminal) Picture loading failed.
P-ALV-B10 Overexpression pGMLV-CMV-MCS-T2A-ZsGreen CMV ZsGreen Null NULL Picture loading failed.
P-ALV-B11 Overexpression pGMLV-CMV-MCS-3flag-EF1-ZsGreen-T2A-Puromycin CMV ZsGreen Puromycin 3FLAG(C-terminal) Picture loading failed.
P-ALV-B12 Overexpression pGMLV-CMV-MCS-EF1-ZsGreen-T2A-Puromycin CMV ZsGreen Puromycin NULL Picture loading failed.
P-ALV-B13 Overexpression pGMLV-CMV-MCS-T2A-Puromycin CMV Null Puromycin NULL Picture loading failed.
P-ALV-B14 Overexpression pGMLV-CMV-MCS-dsRed-PGK-Puromycin CMV dsRed Puromycin NULL Picture loading failed.
P-ALV-B15 Overexpression pGMLV-CMV-MCS-IRES-Puromycin CMV Null Puromycin NULL Picture loading failed.
P-ALV-B16 Overexpression pGMLV-CMV-MCS-IRES-ZsGreen CMV ZsGreen Null NULL Picture loading failed.
P-ALV-B17 Overexpression pGMLV-CMV-MCS-PGK-ZsGreen CMV ZsGreen Null NULL Picture loading failed.
P-ALV-B18 Overexpression pGMLV-CMV-MCS-PGK-Puromycin-IRES-ZsGreen CMV ZsGreen Puromycin NULL Picture loading failed.
P-ALV-B19 shRNA for RNAi pGMLV-U6-MCS-PGK-Puromycin U6 Null Puromycin NULL Picture loading failed.
P-ALV-B20 shRNA for RNAi pGMLV-U6-MCS-CMV-ZsGreen U6 ZsGreen Null NULL Picture loading failed.
P-ALV-B21 shRNA for RNAi pGMLV-U6-MCS-CMV-ZsGreen-PGK-Puromycin U6 ZsGreen Puromycin NULL Picture loading failed.
P-ALV-B22 Overexpression pGMLV-EM7-MCS-Ubc-rtTA3-Puromycin EM7 Null Puromycin NULL Picture loading failed.
P-ALV-B23 Overexpression pGMLV-PGK-Puromycin-Tnl-EGFP PGK EGFP Puromycin NULL Picture loading failed.
P-ALV-B24 Overexpression pGMLV-CMV-MCS-PGK-RFP CMV RFP Null NULL Picture loading failed.
P-ALV-B25 Overexpression pGMLV-CMV-MCS-PGK-Z418 CMV Null Z418 NULL Picture loading failed.
P-ALV-B26 shRNA for RNAi pGMLV-U6-MCS-PGK-BSD U6 Null Blasticidin NULL Picture loading failed.
P-ALV-B27 Overexpression pGMLV-TetOn-SV40-Puromycin-TRE3GS-MCS TRE3GS Null Puromycin NULL Picture loading failed.
P-ALV-B28 shRNA for RNAi pGMLV-U6-MCS-hPGK-Puromycin U6 Null Puromycin NULL Picture loading failed.
P-ALV-B29 Overexpression pGMLV-CMV-MCS-EF1-RFP-T2A-Luc CMV RFP/Luciferase Null NULL Picture loading failed.
P-ALV-B30 Overexpression pGMLV-CMV-MCS-EF1-RFP-T2A-Puromycin CMV RFP Puromycin NULL Picture loading failed.
P-ALV-B31 Overexpression pGMLV-CMV-MCS-EF1-ZsGreen-T2A-Luc CMV ZsGreen
/Luciferase
Null NULL Picture loading failed.
P-ALV-B32 shRNA for RNAi pGMLV-U6-MCS-EF1-Luc-T2A-Puromycin U6 Luciferase Puromycin NULL Picture loading failed.
P-ALV-B33 shRNA for RNAi pGMLV-U6-MCS-EF1-RFP-T2A-Luc U6 RFP
/Luciferase
Null NULL Picture loading failed.
P-ALV-B34 shRNA for RNAi pGMLV-U6-MCS-EF1-RFP-T2A-Puromycin U6 RFP Puromycin NULL Picture loading failed.
P-ALV-B35 shRNA for RNAi pGMLV-U6-MCS-EF1-ZsGreen-T2A-Luc U6 ZsGreen
/Luciferase
Null NULL Picture loading failed.
P-ALV-B36 shRNA for RNAi pGMLV-U6-MCS-EF1-ZsGreen-T2A-BSD U6 ZsGreen Blasticidin NULL Picture loading failed.
P-ALV-B37 Overexpression pGMLV-CMV-MCS-EF1-ZsGreen-T2A-BSD CMV ZsGreen Blasticidin NULL Picture loading failed.
P-ALV-B38 shRNA for RNAi pGMLV-U6-MCS-EF1-fLuc-T2A-mCherry U6 mCherry
/Luciferase
Null NULL Picture loading failed.
P-ALV-B39 Overexpression pGMLV-CMV-MCS-EF1-fLuc-T2A-mcherry CMV mCherry
/Luciferase
Null NULL Picture loading failed.
P-ALV-B40 shRNA for RNAi pGMLV-U6-MCS-EF1-fLuc-T2A-BSD U6 Luciferase Blasticidin NULL Picture loading failed.
P-ALV-B41 shRNA for RNAi pGMLV-U6-MCS-PGK-Z418 U6 Null Z418 NULL Picture loading failed.
P-ALV-B42 Overexpression pGMLV-CMV-MCS-fLUC-EF1-ZsGreen-T2A-Puromycin CMV ZsGreen
/Luciferase
Puromycin NULL Picture loading failed.
P-ALV-B43 Overexpression pGMLV-CMV-MCS-3flag-EF1-ZsGreen-T2A-Puromycin(I) CMV ZsGreen Puromycin 3FLAG(C-terminal) Picture loading failed.
P-ALV-B44 Overexpression pGMLV-CMV-MCS-EF1-ZsGreen-T2A-fLUC(I) CMV ZsGreen
/Luciferase
Null NULL Picture loading failed.
P-ALV-B45 shRNA for RNAi pGMLV-U6-MCS-CMV-mCherry-P2A-BSD U6 mCherry Blasticidin NULL Picture loading failed.
P-ALV-B46 shRNA for RNAi pGMLV-U6-MCS-CMV-EGFP-P2A-BSD U6 EGFP Blasticidin NULL Picture loading failed.
P-ALV-B47 Overexpression pGMLV-CMV-DIO-ZsGreen-P2A-MCS-DIO-EF1-Puromycin CMV ZsGreen Puromycin NULL Picture loading failed.
P-ALV-B48 Overexpression pGMLV-CMV-MCS-P2A-ZsGreen CMV ZsGreen Null NULL Picture loading failed.
P-ALV-B49 gRNA pGMLV-U6-gRNA-EF1-Puromycin U6 Null Puromycin NULL Picture loading failed.
P-ALV-B50 cas9/gRNA pGMLV-U6-gRNA-EF1-CAS9-Puromycin U6 Null Puromycin 1FLAG Picture loading failed.
P-ALV-B51 shRNA for RNAi pGMLV-U6-MCS-EF1-mCherry-T2A-Puromycin U6 mCherry Puromycin NULL Picture loading failed.
P-ALV-B52 Overexpression pGMLV-CMV-MCS-EF1-ZsGreen CMV ZsGreen Null NULL Picture loading failed.
P-ALV-B53 Overexpression pGMLV-CMV-MCS-3flag-EF1-ZsGreen CMV ZsGreen Null 3FLAG(C-terminal) Picture loading failed.
P-ALV-B54 Overexpression pCDH-MSCV-MCS-3flag-EF1-copGFP-T2A-Puromycin MSCV copGFP Puromycin 3FLAG(C-terminal) Picture loading failed.
P-ALV-B55 gRNA pGMLV-U6-gRNA-EF1-ZsGreen U6 ZsGreen Null NULL Picture loading failed.
P-ALV-B56 Overexpression pGMLV-CMV-MCS-EF1-fLUC-T2A-BSD CMV Luciferase Blasticidin NULL Picture loading failed.
P-ALV-B57 Overexpression pGMLV-CMV-MCS-EF1-Cas9-P2A-BSD CMV Null Blasticidin NULL Picture loading failed.
P-ALV-B58 Overexpression pGMLV-CMV-MCS-EF1-RFP-T2A-Hygro CMV RFP hygro NULL Picture loading failed.
P-ALV-B59 shRNA for RNAi pGMLV-U6-CMV-RFP-P2A-BSD-N U6 RFP Blasticidin NULL Picture loading failed.
P-ALV-B60 Overexpression pGMLV-CMV-EF1-ZsGreen-P2A-BSD CMV ZsGreen Blasticidin NULL Picture loading failed.
P-ALV-B61 Overexpression pGMLV-CMV-MCS-T2A-mCherry CMV mCherry Null NULL Picture loading failed.
P-ALV-B62 shRNA for RNAi pGMLV-U6-CMV-EGFP-P2A-BSD U6 EGFP Blasticidin NULL Picture loading failed.
P-ALV-B63 Overexpression pGMLV-DIO-ZsGreen-P2A-MCS-DIO-Puromycin CMV ZsGreen Puromycin NULL Picture loading failed.
P-ALV-B64 Overexpression pGMLV-CMV-EF1-ZsGreen-P2A-Puromycin CMV ZsGreen Puromycin NULL Picture loading failed.
P-ALV-B65 Overexpression pGMLV-CMV-P2A-ZsGreen-P2A-Puromycin CMV ZsGreen Puromycin NULL Picture loading failed.
P-ALV-B66 Overexpression pGMLV-MCS-EF1-ZsGreen-T2A-Puromycin Null ZsGreen Puromycin NULL Picture loading failed.
P-ALV-B67 LC3 pGMLV-CMV-EGFP-LC3-T2A-Puromycin CMV EGFP Puromycin NULL Picture loading failed.
P-ALV-B68 LC3 pGMLV-CMV-mCherry-EGFP-LC3-T2A-Puromycin CMV EGFP
/mCherry
Puromycin NULL Picture loading failed.
P-ALV-B69 cas9/gRNA pGMLV-U6-scramble-EF1-cas9-Puromycin U6 Null Puromycin NULL Picture loading failed.
P-ALV-B70 cas9/gRNA pGMLV-U6-scramble-CMV-Cas9-T2A-ZsGreen U6 ZsGreen Null NULL Picture loading failed.
P-ALV-B71 cas9/gRNA pGMLV-U6-scramble-EF1-Cas9-P2A-ZsGreen U6 ZsGreen Null NULL Picture loading failed.
P-ALV-B72 Overexpression pGMLV-CMV-MCS-EF1-Z418 CMV Null Z418 NULL Picture loading failed.
P-ALV-B73 Overexpression pGMLV-CMV-MCS-3flag-EF1-mCherry-P2A-BSD CMV mCherry Blasticidin 3FLAG(C-terminal) Picture loading failed.
P-ALV-B74 shRNA for RNAi pGMLV-U6-MCS-PGK-Z418(E) U6 Null Z418 NULL Picture loading failed.
P-ALV-B75 shRNA for RNAi pGMLV-U6-gRNA-PGK-BSD U6 Null Blasticidin NULL Picture loading failed.
P-ALV-B76 Overexpression pGMLV-EF1-T2A-MCS-ZsGreen EF1 ZsGreen Null NULL Picture loading failed.
P-ALV-B77 cas9/gRNA pGMLV-U6-gRNA-EF1-mCherry-BSD U6 mCherry Blasticidin NULL Picture loading failed.
P-ALV-B78 Overexpression pGMLV-CMV-DIO-EF1-Puromycin CMV Null Puromycin NULL Picture loading failed.
P-ALV-B79 Overexpression pGMLV-CMV-Circ-MCS-EF1-ZsGreen CMV ZsGreen Null NULL Picture loading failed.
P-ALV-B80 Overexpression pGMLV-CMV-cre-3flag-EF1-ZsGreen-T2A-Puromycin CMV ZsGreen Puromycin 3FLAG(C-terminal) Picture loading failed.
P-ALV-B81 Overexpression pGMLV-EF1-MCS-CMV-Puromycin EF1 Null Puromycin NULL Picture loading failed.
P-ALV-B82 Overexpression pGMLV-EF1-MCS-CMV-ZsGreen EF1 ZsGreen Null NULL Picture loading failed.
P-ALV-B83 Overexpression pGMLV-EF1-MCS-CMV-ZsGreen-T2A-Puromycin EF1 ZsGreen Puromycin NULL Picture loading failed.
P-ALV-B84 Overexpression pGMLV-EF1-MCS-P2A-ZsGreen-P2A-Puromycin EF1 ZsGreen Puromycin NULL Picture loading failed.
P-ALV-B85 Overexpression pGMLV-CMV-MCS-3flag-EF1-mcherry CMV mCherry Null 3FLAG(C-terminal) Picture loading failed.
P-ALV-B86 Overexpression pGMLV-CMV-MCS-3flag-EF1-eRFP-T2A-Puromycin CMV eRFP Puromycin 3FLAG(C-terminal) Picture loading failed.
P-ALV-B87 shRNA for RNAi pGMLV-U6-MCS-EF1-eRFP-T2A-Puromycin U6 eRFP Puromycin NULL Picture loading failed.
P-ALV-B88 Overexpression pGMLV-CMV-eRFP-EGFP-LC3-T2A-Puromycin CMV eRFP/EGFP Puromycin NULL Picture loading failed.
P-ALV-B89 Overexpression pGMLV-CMV-circ-EF1-copGFP-Puromycin CMV copGFP Puromycin NULL Picture loading failed.
P-ALV-B90 Overexpression pGMLV-CMV-LUC-EF1-eRFP-T2A-Puromycin CMV eRFP
/Luciferase
Puromycin NULL Picture loading failed.
P-ALV-B91 Overexpression pGMLV-CMV-circ-EF1-ZsGreen-T2A-Puromycin-N CMV ZsGreen Puromycin NULL Picture loading failed.



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