Lentivirus vector system (lentivirus expression system, lentivirus packaging plasmid system) (Amp and Kanamycin)
Lentivirus vector system - Introduction
GeneMedi's lentivirus Vector System, also named the lentivirus expression system or lentivirus packaging plasmid system, is a powerful tool for in-vitro & in-vivo gene delivery, shRNA mediated RNA interference (RNAi), gene editing and stable cellline development. You can easily produce a recombinant lentivirus particle in HEK293T or HEK293FT cell line in high titer using GeneMedi's lentivirus Vector System.
The Genemedi lentivirus vector system including 3 lentivirus packaging plasmids: the lentivirus expression plasmids with different promoters and tags, an envelope protein VSV-G expressing plasmid pMD2G and a packaging plasmid pSPAX2 expressing Gag-pol and Tat.
GeneMedi offers both Ampicillin (Amp) resistance and Kanamycin (Kan) resistance for the Lentivirus Vector Systems.
Figure 1: Workflow of lentivirus packaging
GeneMedi has developed a variety of lentivirus expression vectors with different expression cassettes, containing kinds of verified promoters and reporters including GFP, zsgreen, RFP, mcherry and luciferase. The GeneMedi's lentivirus expression vectors have been proved very suitalble for unique gene overexpression or shRNA-mediated knock-down (also called RNAi (RNA interference ). You can also achieve gene knock-out(KO) or gene editing using our Crispr-cas9-gRNA lentivirus expression vector.
Lentivirus vector system (Amp and Kanamycin)
Cat.NO | Product Name | Organization Type | Price(In USD) | ||
---|---|---|---|---|---|
P-PK23 | Lentivirus vector system (Amp resistance) 1. PMD2G, Cat No.: P-ALV-C01; 5ug 2. pSPAX2, Cat No.: P-ALV-C02; 5ug 3. Lentivirus expression vectors: pGMLV-CMV-MCS-EF1-ZsGreen (Cat No.: P-ALV-B52), 5ug (Expression vector refers only to B52, if you need other expression vector, please check the table below) |
Academic | $2,430.00 | ||
P-PK23 | Lentivirus vector system (Amp resistance) 1. PMD2G, Cat No.: P-ALV-C01; 5ug 2. pSPAX2, Cat No.: P-ALV-C02; 5ug 3. Lentivirus expression vectors: pGMLV-CMV-MCS-EF1-ZsGreen (Cat No.: P-ALV-B52), 5ug (Expression vector refers only to B52, if you need other expression vector, please check the table below) |
Industry R&D (excluding CRO&CDMO&CXO) |
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P-PK23 | Lentivirus vector system (Amp resistance) 1. PMD2G, Cat No.: P-ALV-C01; 5ug 2. pSPAX2, Cat No.: P-ALV-C02; 5ug 3. Lentivirus expression vectors: pGMLV-CMV-MCS-EF1-ZsGreen (Cat No.: P-ALV-B52), 5ug (Expression vector refers only to B52, if you need other expression vector, please check the table below) |
CRO&CDMO& CXO&CMC& Manufacturing company |
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P-PK23 | Lentivirus vector system (Amp resistance) 1. PMD2G, Cat No.: P-ALV-C01; ≥10mg 2. pSPAX2, Cat No.: P-ALV-C02; ≥10mg 3. Lentivirus expression vectors: pGMLV-CMV-MCS-EF1-ZsGreen (Cat No.: P-ALV-B52), ≥10mg (Expression vector refers only to B52, if you need other expression vector, please check the table below) |
All | |||
P-PK23-Kan | Lentivirus vector system (Kan resistance) 1. PMD2G(Kan resistance), Cat No.: P-ALV-C01-Kan; ≥10mg 2. pSPAX2(Kan resistance), Cat No.: P-ALV-C02-Kan; ≥10mg 3. Lentivirus expression vectors: pGMLV-CMV-MCS-EF1-ZsGreen(Kan resistance) (Cat No.: P-ALV-B52-Kan), ≥10mg |
All | |||
GeneMedi offers both seed and amplification services for the Amp-resistant Lentivirus Vector System. GeneMedi only offers amplification services and do not provide seed for the Kan-resistant Lentivirus Vector System currently. | |||||
If you want to obtain higher quantities of plasmids (mg), please visit GM TransExcellent Plasmid DNA Rapid Preparation Service. | |||||
Terms of sale: For Seed (5ug): The purchaser may not reverse engineer this product to extract the sequence for independent use. The purchaser may not transfer this product to others for manufacturing purposes. The purchaser may not use this material to manufacture this product for any other party. For plasmid production amplification (≥10mg): The purchaser may not reverse engineer this product to extract the sequence for independent use. The purchaser may not transfer this product to others for manufacturing purposes. The purchaser may not use this material to manufacture this product for themselves or any other party. |
Note: Industry R&D excludes CRO&CDMO&CXO; Others includes CRO&CDMO&CXO&CMC&Manufacturing company
Helper plasmids of lentivirus packaging (Amp and Kanamycin)
Helper plasmids | PMD2G (Amp resistance) | pSPAX2 (Amp resistance) |
Catalog No. | P-ALV-C01 | P-ALV-C02 |
Description | an envelope protein VSV-G expressing plasmid | lentivirus packaging plasmids expressing Gag-pol and Tat |
Map and sequence | Download the detail and read in Snapgene Software |
Download the detail and read in Snapgene Software |
Click here to download SnapGene Viewer
Cat.No | Product Name | Detail |
P-ALV-C01 | pMD2G; 5ug (seed) / ≥10mg (amplification services) | Details |
P-ALV-C01-Kan | pMD2G (Kan resistance); ≥10mg (amplification services) | Details |
P-ALV-C02 | pSPAX2; 5ug (seed) / ≥10mg (amplification services) | Details |
P-ALV-C02-Kan | pSPAX2 (Kan resistance); ≥10mg (amplification services) | Details |
Lentivirus expression vectors (lentivirus expression plasmids) (Amp and Kanamycin)
Note: Industry R&D excludes CRO&CDMO&CXO; Others includes CRO&CDMO&CXO&CMC&Manufacturing company
Cat.No | Expression | Name of Vectors | Promoter | Reporter | Cre-dependent | TAG (unfusion) | Order |
P-ALV-B01 | Overexpression | pCDH-MSCV-MCS-EF1-copGFP-T2A-Puromycin | MSCV | copGFP | Puromycin | NULL | |
P-ALV-B02 | Overexpression | pGMLV-CMV-Circ-MCS-EF1-ZsGreen-T2A-Puromycin | CMV | ZsGreen | Puromycin | NULL | |
P-ALV-B03 | Overexpression | pGMLV-CMV-MCS-3flag-EF1-fLUC-T2A-Puromycin | CMV | Luciferase | Puromycin | 3FLAG(C-terminal) | |
P-ALV-B04 | Overexpression | pGMLV-CMV-MCS-EF1-fLUC-T2A-Puromycin | CMV | Luciferase | Puromycin | NULL | |
P-ALV-B05 | Overexpression | pGMLV-CMV-MCS-EF1-mCherry-T2A-Puromycin | CMV | mCherry | Puromycin | NULL | |
P-ALV-B06 | Overexpression | pGMLV-CMV-MCS-3flag-EF1-mCherry-T2A-Puromycin | CMV | mCherry | Puromycin | 3FLAG(C-terminal) | |
P-ALV-B07 | Overexpression | pGMLV-CMV-MCS-EF1-Puromycin | CMV | Null | Puromycin | NULL | |
P-ALV-B08 | Overexpression | pGMLV-CMV-MCS-3flag-EF1-Puromycin | CMV | Null | Puromycin | 3FLAG(C-terminal) | |
P-ALV-B09 | Overexpression | pGMLV-CMV-MCS-3flag-T2A-ZsGreen | CMV | ZsGreen | Null | 3FLAG(C-terminal) | |
P-ALV-B10 | Overexpression | pGMLV-CMV-MCS-T2A-ZsGreen | CMV | ZsGreen | Null | NULL | |
P-ALV-B11 | Overexpression | pGMLV-CMV-MCS-3flag-EF1-ZsGreen-T2A-Puromycin | CMV | ZsGreen | Puromycin | 3FLAG(C-terminal) | |
P-ALV-B12 | Overexpression | pGMLV-CMV-MCS-EF1-ZsGreen-T2A-Puromycin | CMV | ZsGreen | Puromycin | NULL | |
P-ALV-B13 | Overexpression | pGMLV-CMV-MCS-T2A-Puromycin | CMV | Null | Puromycin | NULL | |
P-ALV-B14 | Overexpression | pGMLV-CMV-MCS-dsRed-PGK-Puromycin | CMV | dsRed | Puromycin | NULL | |
P-ALV-B15 | Overexpression | pGMLV-CMV-MCS-IRES-Puromycin | CMV | Null | Puromycin | NULL | |
P-ALV-B16 | Overexpression | pGMLV-CMV-MCS-IRES-ZsGreen | CMV | ZsGreen | Null | NULL | |
P-ALV-B17 | Overexpression | pGMLV-CMV-MCS-PGK-ZsGreen | CMV | ZsGreen | Null | NULL | |
P-ALV-B18 | Overexpression | pGMLV-CMV-MCS-PGK-Puromycin-IRES-ZsGreen | CMV | ZsGreen | Puromycin | NULL | |
P-ALV-B19 | shRNA for RNAi | pGMLV-U6-MCS-PGK-Puromycin | U6 | Null | Puromycin | NULL | |
P-ALV-B20 | shRNA for RNAi | pGMLV-U6-MCS-CMV-ZsGreen | U6 | ZsGreen | Null | NULL | |
P-ALV-B21 | shRNA for RNAi | pGMLV-U6-MCS-CMV-ZsGreen-PGK-Puromycin | U6 | ZsGreen | Puromycin | NULL | |
P-ALV-B22 | Overexpression | pGMLV-EM7-MCS-Ubc-rtTA3-Puromycin | EM7 | Null | Puromycin | NULL | |
P-ALV-B23 | Overexpression | pGMLV-PGK-Puromycin-Tnl-EGFP | PGK | EGFP | Puromycin | NULL | |
P-ALV-B24 | Overexpression | pGMLV-CMV-MCS-PGK-RFP | CMV | RFP | Null | NULL | |
P-ALV-B25 | Overexpression | pGMLV-CMV-MCS-PGK-Z418 | CMV | Null | Z418 | NULL | |
P-ALV-B26 | shRNA for RNAi | pGMLV-U6-MCS-PGK-BSD | U6 | Null | Blasticidin | NULL | |
P-ALV-B27 | Overexpression | pGMLV-TetOn-SV40-Puromycin-TRE3GS-MCS | TRE3GS | Null | Puromycin | NULL | |
P-ALV-B28 | shRNA for RNAi | pGMLV-U6-MCS-hPGK-Puromycin | U6 | Null | Puromycin | NULL | |
P-ALV-B29 | Overexpression | pGMLV-CMV-MCS-EF1-RFP-T2A-Luc | CMV | RFP/Luciferase | Null | NULL | |
P-ALV-B30 | Overexpression | pGMLV-CMV-MCS-EF1-RFP-T2A-Puromycin | CMV | RFP | Puromycin | NULL | |
P-ALV-B31 | Overexpression | pGMLV-CMV-MCS-EF1-ZsGreen-T2A-Luc | CMV | ZsGreen /Luciferase |
Null | NULL | |
P-ALV-B32 | shRNA for RNAi | pGMLV-U6-MCS-EF1-Luc-T2A-Puromycin | U6 | Luciferase | Puromycin | NULL | |
P-ALV-B33 | shRNA for RNAi | pGMLV-U6-MCS-EF1-RFP-T2A-Luc | U6 | RFP /Luciferase |
Null | NULL | |
P-ALV-B34 | shRNA for RNAi | pGMLV-U6-MCS-EF1-RFP-T2A-Puromycin | U6 | RFP | Puromycin | NULL | |
P-ALV-B35 | shRNA for RNAi | pGMLV-U6-MCS-EF1-ZsGreen-T2A-Luc | U6 | ZsGreen /Luciferase |
Null | NULL | |
P-ALV-B36 | shRNA for RNAi | pGMLV-U6-MCS-EF1-ZsGreen-T2A-BSD | U6 | ZsGreen | Blasticidin | NULL | |
P-ALV-B37 | Overexpression | pGMLV-CMV-MCS-EF1-ZsGreen-T2A-BSD | CMV | ZsGreen | Blasticidin | NULL | |
P-ALV-B38 | shRNA for RNAi | pGMLV-U6-MCS-EF1-fLuc-T2A-mCherry | U6 | mCherry /Luciferase |
Null | NULL | |
P-ALV-B39 | Overexpression | pGMLV-CMV-MCS-EF1-fLuc-T2A-mcherry | CMV | mCherry /Luciferase |
Null | NULL | |
P-ALV-B40 | shRNA for RNAi | pGMLV-U6-MCS-EF1-fLuc-T2A-BSD | U6 | Luciferase | Blasticidin | NULL | |
P-ALV-B41 | shRNA for RNAi | pGMLV-U6-MCS-PGK-Z418 | U6 | Null | Z418 | NULL | |
P-ALV-B42 | Overexpression | pGMLV-CMV-MCS-fLUC-EF1-ZsGreen-T2A-Puromycin | CMV | ZsGreen /Luciferase |
Puromycin | NULL | |
P-ALV-B43 | Overexpression | pGMLV-CMV-MCS-3flag-EF1-ZsGreen-T2A-Puromycin(I) | CMV | ZsGreen | Puromycin | 3FLAG(C-terminal) | |
P-ALV-B44 | Overexpression | pGMLV-CMV-MCS-EF1-ZsGreen-T2A-fLUC(I) | CMV | ZsGreen /Luciferase |
Null | NULL | |
P-ALV-B45 | shRNA for RNAi | pGMLV-U6-MCS-CMV-mCherry-P2A-BSD | U6 | mCherry | Blasticidin | NULL | |
P-ALV-B46 | shRNA for RNAi | pGMLV-U6-MCS-CMV-EGFP-P2A-BSD | U6 | EGFP | Blasticidin | NULL | |
P-ALV-B47 | Overexpression | pGMLV-CMV-DIO-ZsGreen-P2A-MCS-DIO-EF1-Puromycin | CMV | ZsGreen | Puromycin | NULL | |
P-ALV-B48 | Overexpression | pGMLV-CMV-MCS-P2A-ZsGreen | CMV | ZsGreen | Null | NULL | |
P-ALV-B49 | gRNA | pGMLV-U6-gRNA-EF1-Puromycin | U6 | Null | Puromycin | NULL | |
P-ALV-B50 | cas9/gRNA | pGMLV-U6-gRNA-EF1-CAS9-Puromycin | U6 | Null | Puromycin | 1FLAG | |
P-ALV-B51 | shRNA for RNAi | pGMLV-U6-MCS-EF1-mCherry-T2A-Puromycin | U6 | mCherry | Puromycin | NULL | |
P-ALV-B52 | Overexpression | pGMLV-CMV-MCS-EF1-ZsGreen | CMV | ZsGreen | Null | NULL | |
P-ALV-B53 | Overexpression | pGMLV-CMV-MCS-3flag-EF1-ZsGreen | CMV | ZsGreen | Null | 3FLAG(C-terminal) | |
P-ALV-B54 | Overexpression | pCDH-MSCV-MCS-3flag-EF1-copGFP-T2A-Puromycin | MSCV | copGFP | Puromycin | 3FLAG(C-terminal) | |
P-ALV-B55 | gRNA | pGMLV-U6-gRNA-EF1-ZsGreen | U6 | ZsGreen | Null | NULL | |
P-ALV-B56 | Overexpression | pGMLV-CMV-MCS-EF1-fLUC-T2A-BSD | CMV | Luciferase | Blasticidin | NULL | |
P-ALV-B57 | Overexpression | pGMLV-CMV-MCS-EF1-Cas9-P2A-BSD | CMV | Null | Blasticidin | NULL | |
P-ALV-B58 | Overexpression | pGMLV-CMV-MCS-EF1-RFP-T2A-Hygro | CMV | RFP | hygro | NULL | |
P-ALV-B59 | shRNA for RNAi | pGMLV-U6-CMV-RFP-P2A-BSD-N | U6 | RFP | Blasticidin | NULL | |
P-ALV-B60 | Overexpression | pGMLV-CMV-EF1-ZsGreen-P2A-BSD | CMV | ZsGreen | Blasticidin | NULL | |
P-ALV-B61 | Overexpression | pGMLV-CMV-MCS-T2A-mCherry | CMV | mCherry | Null | NULL | |
P-ALV-B62 | shRNA for RNAi | pGMLV-U6-CMV-EGFP-P2A-BSD | U6 | EGFP | Blasticidin | NULL | |
P-ALV-B63 | Overexpression | pGMLV-DIO-ZsGreen-P2A-MCS-DIO-Puromycin | CMV | ZsGreen | Puromycin | NULL | |
P-ALV-B64 | Overexpression | pGMLV-CMV-EF1-ZsGreen-P2A-Puromycin | CMV | ZsGreen | Puromycin | NULL | |
P-ALV-B65 | Overexpression | pGMLV-CMV-P2A-ZsGreen-P2A-Puromycin | CMV | ZsGreen | Puromycin | NULL | |
P-ALV-B66 | Overexpression | pGMLV-MCS-EF1-ZsGreen-T2A-Puromycin | Null | ZsGreen | Puromycin | NULL | |
P-ALV-B67 | LC3 | pGMLV-CMV-EGFP-LC3-T2A-Puromycin | CMV | EGFP | Puromycin | NULL | |
P-ALV-B68 | LC3 | pGMLV-CMV-mCherry-EGFP-LC3-T2A-Puromycin | CMV | EGFP /mCherry |
Puromycin | NULL | |
P-ALV-B69 | cas9/gRNA | pGMLV-U6-scramble-EF1-cas9-Puromycin | U6 | Null | Puromycin | NULL | |
P-ALV-B70 | cas9/gRNA | pGMLV-U6-scramble-CMV-Cas9-T2A-ZsGreen | U6 | ZsGreen | Null | NULL | |
P-ALV-B71 | cas9/gRNA | pGMLV-U6-scramble-EF1-Cas9-P2A-ZsGreen | U6 | ZsGreen | Null | NULL | |
P-ALV-B72 | Overexpression | pGMLV-CMV-MCS-EF1-Z418 | CMV | Null | Z418 | NULL | |
P-ALV-B73 | Overexpression | pGMLV-CMV-MCS-3flag-EF1-mCherry-P2A-BSD | CMV | mCherry | Blasticidin | 3FLAG(C-terminal) | |
P-ALV-B74 | shRNA for RNAi | pGMLV-U6-MCS-PGK-Z418(E) | U6 | Null | Z418 | NULL | |
P-ALV-B75 | shRNA for RNAi | pGMLV-U6-gRNA-PGK-BSD | U6 | Null | Blasticidin | NULL | |
P-ALV-B76 | Overexpression | pGMLV-EF1-T2A-MCS-ZsGreen | EF1 | ZsGreen | Null | NULL | |
P-ALV-B77 | cas9/gRNA | pGMLV-U6-gRNA-EF1-mCherry-BSD | U6 | mCherry | Blasticidin | NULL | |
P-ALV-B78 | Overexpression | pGMLV-CMV-DIO-EF1-Puromycin | CMV | Null | Puromycin | NULL | |
P-ALV-B79 | Overexpression | pGMLV-CMV-Circ-MCS-EF1-ZsGreen | CMV | ZsGreen | Null | NULL | |
P-ALV-B80 | Overexpression | pGMLV-CMV-cre-3flag-EF1-ZsGreen-T2A-Puromycin | CMV | ZsGreen | Puromycin | 3FLAG(C-terminal) | |
P-ALV-B81 | Overexpression | pGMLV-EF1-MCS-CMV-Puromycin | EF1 | Null | Puromycin | NULL | |
P-ALV-B82 | Overexpression | pGMLV-EF1-MCS-CMV-ZsGreen | EF1 | ZsGreen | Null | NULL | |
P-ALV-B83 | Overexpression | pGMLV-EF1-MCS-CMV-ZsGreen-T2A-Puromycin | EF1 | ZsGreen | Puromycin | NULL | |
P-ALV-B84 | Overexpression | pGMLV-EF1-MCS-P2A-ZsGreen-P2A-Puromycin | EF1 | ZsGreen | Puromycin | NULL | |
P-ALV-B85 | Overexpression | pGMLV-CMV-MCS-3flag-EF1-mcherry | CMV | mCherry | Null | 3FLAG(C-terminal) | |
P-ALV-B86 | Overexpression | pGMLV-CMV-MCS-3flag-EF1-eRFP-T2A-Puromycin | CMV | eRFP | Puromycin | 3FLAG(C-terminal) | |
P-ALV-B87 | shRNA for RNAi | pGMLV-U6-MCS-EF1-eRFP-T2A-Puromycin | U6 | eRFP | Puromycin | NULL | |
P-ALV-B88 | Overexpression | pGMLV-CMV-eRFP-EGFP-LC3-T2A-Puromycin | CMV | eRFP/EGFP | Puromycin | NULL | |
P-ALV-B89 | Overexpression | pGMLV-CMV-circ-EF1-copGFP-Puromycin | CMV | copGFP | Puromycin | NULL | |
P-ALV-B90 | Overexpression | pGMLV-CMV-LUC-EF1-eRFP-T2A-Puromycin | CMV | eRFP /Luciferase |
Puromycin | NULL | |
P-ALV-B91 | Overexpression | pGMLV-CMV-circ-EF1-ZsGreen-T2A-Puromycin-N | CMV | ZsGreen | Puromycin | NULL |