Advanced Adenovirus Production & Gene Delivery Services

  • Product Overview
  • Manuals & MSDS
  • FAQs
  • Citations and references
  • Recommended products

Order Information

  • Step 1:
    Enter gene information
  • Step 2:
    ADV vector construction
  • Step 3:
    ADV packaging
  • Step 4:
    Custom Order confirmation
If you only konw the gene sequence, please contact us for the quotation

Note: The inserted gene length should be no longer than 3000 bp in ADV vectors to guarantee high ADV titer. If larger gene is extraordinary necessary, please contact us.

Detail
Note: If the selection of ADV plasmid elements listed above do not fully meet your requirements, please contact us.

Catalog No. Product Lead time(workday) Price
Note: If the selection of ADV serotype and viral packages listed above do not fully meet your requirements, please contact us.

Buy ADV Vector Production Scale Final Deliverables Lead Time(workday) Price(in USD)
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Introduction to Custom Adenovirus Production Service

Recombinant adenovirus (Adenovirus), a replication-defective adenoviral vector system, is widely used for gene delivery in most cell types. The adenoviral vectors provided by Genemedi are based on human adenovirus type 5 (Ad5), which is replication-incompetent (-E1/-E3) and can’t be integrated into host genome, guaranteeing the security for subsequent operations. Adenovirus packaging by Genemedi shows almost 100% gene delivery in most cell types in the recombinant protein expression system both in vivo and in vitro. Recombinant protein expression of interest is detectable from 24 hours post infection. Genemedi has launched a comprehensive adenovirus production service, including a variety of different adenoviral expression systems used for gene over-expression and shRNA mediated gene down-regulation, which may also allow researchers to produce adenoviruses by themselves as they need.

Properties

Adenovirus Vector
Quantity/Unit Vials.
Form Frozen form.
Sipping and Storage Guidelines Shipped by dry ice, stored at -80 ° C, effective for 1 year. Avoid repeatedly freezing and thawing.
Titer > 1*10^10 PFU/ml.

Advantages

1. Broad range of host. Adenovirus has the ability to infect dividing, quiescent cells, stem cells, and primary cells, allowing genetic materials to be delivered to a highly diverse range of cell types and tissues.

2. High infection efficiency. Almost 100% gene delivery in most cell types, completely surpassing other viral vector tools and liposome transfection.

3. Great packaging capacity, up to 8kb.

4. Without integration. No alteration to host genome.

5. High titer. Up to 1011 pfu/ml.

Applications and Figures

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Figure 1
Transduction results of various Adenovirus vectors. Selected results of Adenovirus transduction in the adult rat cardiac cells (A), eyes (B), neuron cells (C).

Quality control description

Our optimized custom adenoviral vector production and strict quality control systems provide customers a high titer of functional recombinant adenoviral vectors. Viral titers are determined by TCID50 method, which is the most accurate way to measure the titer of adenovirus.

Technical Documents

1. For detailed protocol about how to package and purify Adenovirus, please see the pdf downloadAdenovirus User Manual on the Genemedi website.

Frequently Asked Questions(FAQs)

1. Why does adenovirus have a relatively higher immunogenecity compared with rAAV?
Answer

The adenovirus without E1/E3 can express all of the other genes in the viral backbone and hence induces immunogenic responses, while rAAV does not have any of the AAV genes, thus no immunogenicity from viral protein.

2. What is the role of the E1 Gene in adenoviruses?
Answer

Simply, the E1 gene products are early proteins that are transcribed in the early transcribed regions and required for proceeding subsequent steps in viral replication. The E1 gene contains E1A and E1B, involved in the replication of adenovirus. E1A is critical to start viral replication by promoting transcription from rep gene promoters, P5 and P19, and facilitate viral replication by activating the early adenovirus promoters.

3. How to store adenovirus?
Answer

It would be better to store adenovirus in PBS at -80oC. Sucrose or DMSO may help to stabilize the vector.

4. How can you tell if your vector is lentiviral, retroviral, or adenoviral?
Answer

You should blast your vector sequence and see if there’re sequences of reverse transcriptase and integrase (gene names: gag and pol), which are for lentiviral/retroviral vectors, but not for adenoviral. For another, if your plasmid is around 30-35kb in size, it's certainly adenoviral.

5. Is adenovirus a useful tool to study primary macrophage functions?
Answer

In RAW264.7 and PM cells, adenovirus works very well, and it seems that IL1β expression is increased slightly after adenovirus transfection compared with negative control. While for the BMDM, adenovirus does not work, it may be better to use lentivirus instead, which gives a pretty good transduction efficacy and less inflammatory response.

6. Is it possible to infect a tissue preparation with lentivirus and afterwards with adenovirus and getting high efficiency in transduction?
Answer

It is definitively possible to perform sequential transductions/infections. Polybreen, protamine sulfate or other transduction enhancing reagents are recommended to enhance viral particle infectivity.

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Reference

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3. Wang Y, X Zhao, X Wu, Y Dai, P Chen and L Xie. (2016). microRNA-182 Mediates Sirt1-Induced Diabetic Corneal Nerve Regeneration. Diabetes 65:2020-31.
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6. Liu H, S Fang, W Wang, Y Cheng, Y Zhang, H Liao, H Yao and J Chao. (2016). Macrophage-derived MCPIP1 mediates silica-induced pulmonary fibrosis via autophagy. Part Fibre Toxicol 13:55.
7. Yao T, X Ying, Y Zhao, A Yuan, Q He, H Tong, S Ding, J Liu, X Peng, E Gao, J Pu and B He. (2015). Vitamin D receptor activation protects against myocardial reperfusion injury through inhibition of apoptosis and modulation of autophagy. Antioxid Redox Signal 22:633-50.
8. Wang B, A Ma, L Zhang, WL Jin, Y Qian, G Xu, B Qiu, Z Yang, Y Liu, Q Xia and Y Liu. (2015). POH1 deubiquitylates and stabilizes E2F1 to promote tumour formation. Nat Commun 6:8704.
9. Hua F, K Li, JJ Yu, XX Lv, J Yan, XW Zhang, W Sun, H Lin, S Shang, F Wang, B Cui, R Mu, B Huang, JD Jiang and ZW Hu. (2015). TRB3 links insulin/IGF to tumour promotion by interacting with p62 and impeding autophagic/proteasomal degradations. Nat Commun 6:7951.
10. Wang X, J Liu, J Zhen, C Zhang, Q Wan, G Liu, X Wei, Y Zhang, Z Wang, H Han, H Xu, C Bao, Z Song, X Zhang, N Li and F Yi. (2014). Histone deacetylase 4 selectively contributes to podocyte injury in diabetic nephropathy. Kidney Int 86:712-25.