GM TransExcellent Plasmid DNA Rapid Preparation Service

--1 week, higher efficiency, good for your proteins, antibodies, and viral vector production

GeneMedi offers a plasmid production&amplification service called GM TransExcellent Plasmid DNA Rapid Preparation Service for DNA vector preparation based on the G-NEXT™ platform, which is a fast (1 week), high-quality, high-efficiency, and cost-effective plasmid production&amplification platform and is good for your proteins, antibodies, and viral vector production.

GeneMedi ensures the quality of the final products through strict adherence to standard operating procedures, perfect quality management, strict testing systems, and traceable operating procedures. The platform's large-scale industrial fermentation and fully automated purification process production can accommodate the service needs of industrial users for large-scale plasmid preparation. Furthermore, GeneMedi's Novel Evolution-X Technology (G-NEXT™) is used to explore Gene&Cell therapy (GCT) for more possibilities with innovational strategies.

• How to order
• Validation data
• Plasmid source
• Quality Levels
• Talk with our expert for quote
• Plasmid preparation period
• Production steps

How to order

To place an order for GM TransExcellent Plasmid DNA Rapid Preparation Service, please follow the steps below:

STEP 1: Confirm the necessary information as follows:

a. Confirm the plasmid:
If you are using/will use GENEMEDI's vector system product, please provide us with the Catalog No.(Click here to check the Cat No.)
If you want to use your own plasmid, please provide us with the sequence. After placing your order, please send us 2ug of plasmid.

b. Confirm the Quality Levels of Plasmid DNA:
choose from Research Grade, Endotoxin-Free Grade or High Quality (HQ) Grade by checking out the detail of different levels.

c. Confirm the Plasmid quantity:
choose 10mg or more by checking the period of different quantity.

STEP 2: Submit the confirmed information using the format below:
Cat No.: P-RC13
Quality Level: Research Grade
Plasmid quantity: 10mg
Submit

Submit Example:


You can also send the required information via email to ([email protected]).
Our team will process your request within 1 business day and guide you through the ordering process.

Terms of sale:
The purchaser may not reverse engineer this product to extract the sequence for independent use. The purchaser may not transfer this product to others for manufacturing purposes. The purchaser may not use this material to manufacture this product for themselves or any other party.

Validation data

Plasmid source: GeneMedi's vector system products & Your own plasmid

1.GeneMedi's vector system products includes:

Products	Details
AAV vector system (AAV expression system, AAV packaging plasmid system)	Serotype: AAV1, AAV2, AAV2, AAV2 variant (Y444F), AAV2 variant (Y272F, Y444F, Y500F, Y730F), AAV2 variant (Y444F, Y730F, Y500F, Y272F, Y704F, Y252F), AAV2.7m8, AAV5, AAV6, AAV8, AAV8-1m, AAV8-2m, AAV8-3m, AAV9, AAV-Rh10, AAV-DJ, AAV-DJ8, AAV2-Retro(Retrograde), AAV-PHP.B, AAV-PHP.eB, AAV-PHP.S, AAV-BR1, AAV-VEC, AAV-2i8, AAV-SIG, AAV4, AAV6.2, AAV6.2FF, MyoAAV 1A, MyoAAV 2A, MyoAAV 3A, MyoAAV 4A, MyoAAV 4E, AAV-Anc80, AAV-rh74. AAV-LK03
Adenovirus expression vectors (adenovirus expression plasmids)
Lentivirus vector system	Including PMD2G(Cat No.: P-ALV-C01), pSPAX2(Cat No.: P-ALV-C02) and other expression vector
Promise-ORF™ viral CDNA library	More than 60,000 pre-made CDNA/ORF viral plasmids and viral particles in Lentivirus, Adenovirus and AAV

2.Your own plasmid

Quality Levels of Plasmid DNA

GeneMedi provides three types of Quality Levels of Plasmid DNA: Research Grade, Endotoxin-Free Grade, and High Quality (HQ) Grade. The provided plasmids are compared in Table 1.

QC Item	Service Grades
	Research Grade	Endotoxin-Free Grade	High Quality (HQ) Grade
E.coli Master Cell Bank generation	-	-	Optional
Manufacturing via shake flask growth or high-density fermentation tank	shake flask growth or high-density fermentation tank	shake flask growth or high-density fermentation tank	high-density fermentation tank
Screening for optimal growth conditions	-	-	√
Alkaline lysis	√	√	√
Purification method	Anion exchange chromatography	Anion exchange chromatography, Specialized removal of endotoxins	Gel filtration chromatography, Affinity chromatography, Anion exchange chromatography,Specialized removal of endotoxins
Appearance	√	√	√
pH	-	-	√
A260:280	√	√	√
Residual RNA	Non-detectable by gel electrophoresis at 200ng
Residual E.coli DNA	Non-detectable by gel electrophoresis		Quantitative PCR ≤5%
Residual Host Protein	-	-	HCP ELISA ≤ 1%
Homogeneity	Predominantly supercoiled	≥90% supercoiled
Animal Free Production	Optional	Optional	Optional
Enzyme Free production	-	-	Optional
Restriction Analysis	-	√	√
Identity-Full Insert Sequencing	√	√	√
Endotoxin	-	Gel clot LAL assay < 10EU/mg	Quantitative LAL assay < 5EU/mg
Bioburden Testing	√	√	√
Mycoplasma Contamination	-	-	Optional
Detection of Kanamycin	-	-	Optional
Material Archiving	-	-	Optional
TSE/BSE Certificate	-	-	√
Certificate of Analysis	√	√	√
Consistent Manufacturing Process	√	√	√
Quality oversight	-	-	√

Table 1. Comparation of different grades of Plasmids GeneMedi provides.

Plasmid preparation period

GeneMedi has a fast delivery period advantage, and below are the Preparation periods of different grades of Plasmids provided in Table 2.

Plasmid Grade	Plasmid quantity	Period (working days)
Research Grade	10mg	５～10
	50mg	7～12
	100mg	10～15
	500mg	15～20
	≥1g	Inquiry
	Other quantity	Inquiry
Endotoxin-Free Grade	10mg	５～10
	50mg	10～15
	100mg	15～20
	500mg	20～25
	≥1g	Inquiry
	Other quantity	Inquiry
High Quality (HQ) Grade	Inquiry	Inquiry

Table 2. Preparation periods of different grades of Plasmids GeneMedi provides.

Production steps of Plasmids

Large-scale plasmid DNA manufacturing includes five main steps, as shown in Figure 1.

1. E. coli cells used for plasmid DNA manufacturing were obtained from sources such as DSMZ (German Collection of Microorganisms and Cell Cultures). The transformed cell clones are tested for plasmid identity by restriction analysis, and—if requested—further analysis is applied.

2. A pre-culture of approx. 50 mL is used to inoculate the main culture of 30 L. Cells are cultivated overnight at typically 37 °C or—if necessary to maintain unstable sequences or desired by the customer—at lower temperatures. Cells are harvested on this scale by batch centrifugation.

3. Alkaline lysis is performed by a rapid alka_x0002_line extraction procedure, as published earlier. Significant modification is necessary to scale up this step, as discussed later. Removal of residual particles, cells, or cell fragments is performed by batch centrifugation and multiple filtration steps, including a final 0.2 μm filtration of the “cleared lysate”.

4. Chromatographic steps are performed to reduce the volume (chromatography) and to remove (additional chromatography) contaminating LPS endotoxins, RNA, proteins, and undesired DNA contaminants (chromosomal bacterial DNA, non-supercoiled plasmid forms). The final eluate is filtered (0.2 μm), precipitated, washed, and reconstituted within the buffer.

5. According to the requested aliquots, certified cryotubes are manually filled within a laminar airflow. Vials are labeled and stored under quarantine until the product is released after QC at approx. −20 °C.

Figure 1. Production steps in large-scale plasmid DNA manufacturing from plasmid DNA construct to final product.