Optogenetics Adeno Associated Virus (AAV) hsyn T2A EYFP  

Introduction to Optogenetics Adeno Associated Virus (AAV) Products

Optogenetics
Optogenetics has been a powerful scientific tool to further the fundamental scientific understanding of how specific cell types contribute to the function of biological tissues, such as neural circuits in vivo and lead to new insights into Parkinson's disease and other neurological and psychiatric disorders in clinic. By delivering optical signals to precisely control biological processes in living tissues, optogenetics opens up new landscapes for the study of biology, both in health and disease. Optogenetics is now typically used in neuroscience to control electrical potentials in neurons in vivo.

Adeno associated virus (AAV)
Adeno associated virus (AAV) is one kind of human parvovirus. Recombinant AAV vectors can efficiently transfect various cell types, including dividing and quiescent cells, and induce persistent gene expression in vivo without integrating into host genome and causing any disease. These features make AAV an attractive candidate in the application of gene delivery for gene therapy and human disease model establishment. To date, AAV has been proved as the most excellent gene therapy vector. Over 204 clinical trials have been carried out using AAV vectors for gene delivery, and promising gene therapy outcomes have been achieved from clinical trials for a great number of diseases.

Genemedi optogenetics AAV
Genemedi possesses a complete library of optogenetics components into AAV plasmid. These optogenetics AAV can help researchers control and monitor the activities of individual neurons in living tissue, and precisely measure the manipulation effects in real-time.

Optogenetics Adeno Associated Virus (AAV) premade products list

Cat No.	Products Name	Serotypes of AAV	Order
pGM-AVP001	pGM-AAV-CAG-DIO-EYFP	GeneMedi offer multiple serotypes of AAV for your option: · AAV1 · AAV2 · AAV2 variant (Y444F) · AAV2 variant (Y272F, Y444F, Y500F, Y730F) · AAV2 variant (Y444F, Y730F, Y500F, Y272F, Y704F, Y252F) · AAV2 variant(AAV2.7m8) · AAV5 · AAV6 · AAV8 · AAV8-1m · AAV8-2m · AAV8 variant (Y733F, Y447F, Y275) · AAV9 · AAV-Rh.10 · AAV-DJ · AAV-DJ/8 · AAV-Retro (Retrograde) · AAV9-PHP.B · AAV9-PHP.A · AAV9-PHP.eB · AAV9-PHP.S Please click here to tell us your choice of AAV serotype when you place the order. >>
pGM-AVP002	pGM-AAV-CAG-DIO-eNpHR3.0-EYFP
pGM-AVP003	pGM-AAV-CAG-DIO-hChR2(H134R)-T2A-mcherry
pGM-AVP004	pGM-AAV-CAG-DIO-Arch3.0-EYFP
pGM-AVP005	pGM-AAV-CAG-DIO-hCHETA-EYFP
pGM-AVP006	pGM-AAV-CAG-DIO-hM4Di-mcherry
pGM-AVP007	pGM-AAV-CAG-DIO-hM3DQ-mcherry
pGM-AVP008	pGM-AAV-CAG-DIO-DTR-T2A-mcherry
pGM-AVP009	pGM-AAV-hsyn-T2A-EYFP
pGM-AVP010	pGM-AAV-hsyn-Arch3.0-EYFP
pGM-AVP011	pGM-AAV-hsyn-hCHETA-EYFP
pGM-AVP012	pGM-AAV-hsyn-DIO-hM3DQ-mcherry
pGM-AVP013	pGM-AAV-hsyn-DIO-hM4Di-mcherry
pGM-AVP014	pGM-AAV-hsyn-eNpHR3.0-EYFP
pGM-AVP015	pGM-AAV-hsyn-hChR2(H134R)-mcherry
pGM-AVP016	pGM-AAV-hsyn-hM3DQ-T2A-mcherry
pGM-AVP017	pGM-AAV-hsyn-hM4Di-T2A-mcherry
pGM-AVP018	pGM-AAV-hsyn-DTR-T2A-RFP
pGM-AVP019	pGM-AAV-hsyn-DTA-T2A-mcherry
pGM-AVP020	pGM-AAV-CamkII-EYFP
pGM-AVP021	pGM-AAV-CamkII-eNpHR3.0-EYFP
pGM-AVP022	pGM-AAV-CamkII-hChR2(H134R)-mcherry
pGM-AVP023	pGM-AAV-CamkII-Arch3.0-EYFP
pGM-AVP024	pGM-AAV-CamkII-hCHETA-EYFP
pGM-AVP025	pGM-AAV-CamkII-DTR-T2A-mcherry
pGM-AVP026	pGM-AAV-CamkII-hM3DQ-T2A-mcherry
pGM-AVP027	pGM-AAV-CamkII-hM4Di-T2A-mcherry
pGM-AVP028	pGM-AAV-CamkII-DIO-hM3DQ-mcherry
pGM-AVP029	pGM-AAV-CamkII-DIO-hM4Di-mcherry
pGM-AVP030	pGM-AAV-CAG-DIO-Gcamp6s
pGM-AVP031	pGM-AAV-CAG-DIO-Gcamp6f
pGM-AVP032	pGM-AAV-hsyn-Gcamp6s
pGM-AVP033	pGM-AAV-hsyn-Gcamp6f
pGM-AVP034	pGM-AAV-CamkII-Gcamp6s
pGM-AVP035	pGM-AAV-CamkII-Gcamp6f
pGM-AVP049	pGM-AAV-GFAP-hM4D(Gi)-mCherry
pGM-AVP050	pGM-AAV-GFAP-hM3D(Gq)-mCherry
pGM-AVP051	pGM-AAV-CAG-T2A-EYFP
pGM-AVP052	pGM-AAV-GFAP-eNpHR3.0-EYFP
pGM-AVP053	pGM-AAV-GFAP-hChR2(H134R)-mcherry
pGM-AVP054	pGM-AAV-GFAP-Arch3.0-EYFP
pGM-AVP055	pGM-AAV-GFAP-hCHETA-EYFP
pGM-AVP056	pGM-AAV-GFAP-DTR-T2A-mcherry
pGM-AVP057	pGM-AAV-CAG-DIO-C1V1(t/t)-TS-mcherry
pGM-AVP058	pGM-AAV-CMV-DIO-C1V1(t/t)-TS-mcherry
pGM-AVP059	pGM-AAV-hSyn-DIO-C1V1(t/t)-TS-mcherry
pGM-AVP060	pGM-AAV-GFAP-DIO-C1V1(t/t)-TS-mcherry
pGM-AVP061	pGM-AAV-CaMKII-DIO-C1V1(t/t)-TS-mcherry
pGM-AVP062	pGM-AAV-GFAP-DIO-C1V1(t/t)-TS-mcherry
pGM-AVP063	pGM-AAV-GFAP-ArchT-EYFP
pGM-AVP064	pGM-AAV-CAG-ArchT-EYFP
pGM-AVP065	pGM-AAV-CMV-ArchT-EYFP
pGM-AVP066	pGM-AAV-hSyn-ArchT-EYFP
pGM-AVP067	pGM-AAV-CaMKII-ArchT-EYFP

Applications and Figures

Figure 1.Combination of optogenetics with AAV to determine spontaneous electric activity of nerve in Genemedi.



Figure 2.Selected transduction results of various AAV optogenetics system, such as mice brain tissue (A), rat brain tissue (B), mice brain stem (C), hippocampus (D).