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AAV Helper Plasmid (Amp and Kanamycin) (GeneMedi owned)
AAV protocol Download
AAV User Manual Download
AAV Helper Plasmid - Introduction
GeneMedi offer ready-to-use AAV helper plasmid that is optimized for recombinant Adeno-associated virus (rAAV) packaging, production, and manufacturing within the adenovirus (Adv) helper virus-free system. GeneMedi offers both Ampicillin (Amp) resistance and Kanamycin (Kan) resistance for the AAV helper Plasmid.
The GeneMedi AAV helper plasmid vector contains a mini adenovirus genome that is necessary for recombinant AAV (rAAV) packaging regardless of AAV Rep and Cap genes1,2. The genes consisting mini adenovirus genome in the AAV helper plasmid are described as below:
1. The adenovirus E4 gene for AAV DNA replication
2. The adenovirus E2a gene and adenovirus VA RNA (virus-associated RNA ) genes: enhancing AAV mRNA stability, facilitating AAV gene translation, especially for AAV Capsids transcripts.
GeneMedi offers both Ampicillin (Amp) resistance and Kanamycin (Kan) resistance for the AAV helper Plasmid.
Map and sequence of AAV Helper Plasmid
Download the detail information of AAV Helper Plasmid here and read in Snapgene Software.
Click here to download SnapGene Viewer
Order AAV Helper Plasmid
| Product Name | Organization Type | Price(In USD) | Qty (Quantity) | Sum(In USD) |
|---|---|---|---|---|
| AAV Helper Plasmid Cat.No.:P-HP01 Size: 5ug |
Academic | 504 | ||
| AAV Helper Plasmid Cat.No.:P-HP01 Size: 5ug |
Industry R&D (excluding CRO&CDMO&CXO) |
Inquiry | ||
| AAV Helper Plasmid Cat.No.:P-HP01 Size: 5ug |
CRO&CDMO& CXO&CMC& Manufacturing company |
Inquiry | ||
| AAV Helper Plasmid (Kan resistance) Cat.No.:P-HP01-Kan Size: 10mg *Currently, GeneMedi only offers amplification services for the Kan-resistant AAV Helper Plasmids and do not provide seed stocks. |
All | Inquiry | ||
| Shipping Cost: | 169.00 | |||
| Total: | ||||
| Terms of sale: For Seed (5ug): The purchaser may not reverse engineer this product to extract the sequence for independent use. The purchaser may not transfer this product to others for manufacturing purposes. The purchaser may not use this material to manufacture this product for any other party. For plasmid production&lification (≥10mg): The purchaser may not reverse engineer this product to extract the sequence for independent use. The purchaser may not transfer this product to others for manufacturing purposes. The purchaser may not use this material to manufacture this product for themselves or any other party. |
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References
1. Matsushita, T. et al. Adeno-associated virus vectors can be efficiently produced without helper virus. Gene Therapy 5, 938-945, doi:10.1038/sj.gt.3300680 (1998). Download article PDF
2. Xiao, X., Li, J. & Samulski, R. J. Production of High-Titer Recombinant Adeno-Associated Virus Vectors in the Absence of Helper Adenovirus. JOURNAL OF VIROLOGY 72, 2224-2232 (1998). Download article PDF
Related Products
GeneMedi's AAV vector system
GeneMedi offers AAV(Adeno-Associated Virus) vector system and AAV Vector Systems-Kanamycin Resistance (AAVKan system), the serotype includes:
| Common Serotypes | AAV1, AAV2, AAV4, AAV5, AAV6, AAV6.2, AAV6.2FF, AAV8, AAV9, AAV-Rh.10, AAV-DJ, AAV-Retro (Retrograde), AAV-PHP.B, AAV-PHP.eB, AAV-PHP.S, AAV-BR1, AAV-2i8, AAV-SIG, AAV-VEC. |
| AAV2 Variants | AAV2 variant (Y444F), AAV2 variant (Y272F, Y444F, Y500F, Y730F), AAV2 variant (Y444F, Y730F, Y500F, Y272F, Y704F, Y252F), AAV2 variant (AAV2.7m8). |
| AAV8 Variants | AAV8-1m, AAV8-2m, AAV8 variant (Y733F, Y447F, Y275). |
| MyoAAV Series | AAV-MyoAAV1A, AAV-MyoAAV2A, AAV-MyoAAV3A, AAV-MyoAAV4A, AAV-MyoAAV4E. |
| Other Specialized Variants | AAV-Anc80, AAV-rh74., AAV-LK03 |
GM TransExcellent Plasmid DNA Rapid Preparation Service
GeneMedi offers a plasmid production&lification service called GM TransExcellent Plasmid DNA Rapid Preparation Service for DNA vector preparation based on the G-NEXT™ platform, which is a fast (1 week), high-quality, high-efficiency, and cost-effective plasmid production&lification platform and is good for your proteins, antibodies, and viral vector production. More Details About the Service >>
Knowledge bases
View Adeno-associated virus (AAV vector) Knowledge Base>>
AAV Knowledge Base CRISPR/Cas9-gRNA system Knowledge Base |
Adenovirus Knowledge Base Transfection Knowledge Base |
Lentivirus Knowledge Base Advanced cell therapy |
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