Diagnostic anti-Bovine viral diarrhea virus (BVDV) gp55 antibodies pairs and antigens for animal health (animal infectious disease ) testing in ELISA test,competitive ELISA test, blocking ELISA test, Lateral flow immunoassay (LFIA), colloidal gold immunochromatographic assay, Chemiluminescent immunoassay (CLIA), turbidimetric inhibition immuno assay (TINIA), and immunonephelometry

Definition of the disease: A 1.7 kb cDNA fragment corresponding to the coding region of the major envelope glycoprotein (gp55) of pestivirus hog cholera (Weybridge) was obtained using the polymerase chain reaction (PCR), and then cloned into pUC 8. The deduced amino acid sequence of gp55 showed a strong homology to that of HCV strains Brescia (94%) and Alfort (90%), and to a lesser extent to the closely related gp53 of bovine viral diarrhoea virus strain, NADL (65%). Eighteen cysteine residues were identified in the sequenced region, all of which were conserved between the gp55/gp53 sequences. This suggest that although the homology at the protein level may vary, there are strong conformational motifs which are conserved among the pestivirus envelope proteins.

Genemedi produces core animal health diagnostic ingredients-validated antibodies pairs Mouse anti-Bovine viral diarrhea virus (BVDV) gp55 monoclonal antibody and Bovine viral diarrhea virus (BVDV) gp55 antigens for rapid test kit of animal infectious disease with Bovine viral diarrhea virus (BVDV) gp55 to evaluate the animal health of Ruminants.

The paired antibodies are both monoclonal antibody(mab).

All the antibodies and antiges of animal disease test are suitable for in functional ELISA, and other immunoassays in dignostics.The antibody can act as a capture antibody and detection antibody. Antigens are validated as positive control materials.

Order information

Catalog No. (1~4, 4 antibodies in pairs)	Size	Price(In USD)	Qty (Quantity)	Sum(In USD)
GMP-AD-Ruminants-14Ab-1	Size:1mg	3090
GMP-AD-Ruminants-14Ab-1	Size:10mg	21935
GMP-AD-Ruminants-14Ab-1	Size:100mg	148000
GMP-AD-Ruminants-14Ab-2	Size:1mg	3090
GMP-AD-Ruminants-14Ab-2	Size:10mg	21935
GMP-AD-Ruminants-14Ab-2	Size:100mg	148000
GMP-AD-Ruminants-14Ab-3	Size:1mg	3090
GMP-AD-Ruminants-14Ab-3	Size:10mg	21935
GMP-AD-Ruminants-14Ab-3	Size:100mg	148000
GMP-AD-Ruminants-14Ab-4	Size:1mg	3090
GMP-AD-Ruminants-14Ab-4	Size:10mg	21935
GMP-AD-Ruminants-14Ab-4	Size:100mg	148000
GMP-AD-Ruminants-14Ag-1	Size:1mg	3090
GMP-AD-Ruminants-14Ag-1	Size:10mg	21935
GMP-AD-Ruminants-14Ag-1	Size:100mg	148000
GMP-AD-Ruminants-14Ag-2	Size:1mg	3090
GMP-AD-Ruminants-14Ag-2	Size:10mg	21935
GMP-AD-Ruminants-14Ag-2	Size:100mg	148000
			Shipping Cost:	760.00
			Total:

Description

GMP-AD-Ruminants-14Ab, GMP-AD-Ruminants-14Ag

Cat No.	GMP-AD-Ruminants-14Ab
Antigens	Bovine viral diarrhea virus (BVDV) gp55
Antibody	Mouse anti-Bovine viral diarrhea virus (BVDV) gp55 monoclonal antibody
Resource (expression host)	hybridoma
Specics/Isotypes	Mouse IgG
Bioactivity validation	Antibody Binding, Immunogen in Sandwich Elisa, lateral-flow tests,and other immunoassays in Bovine viral diarrhea virus (BVDV) gp55 level test and Ruminants-diagnositcs.
Antigen description	Bovine leukemia virus (BLV), the etiological agent of enzootic bovine leukosis (EBL), is a C-type retrovirus of cattle with a worldwide distribution (5). The virus induces a persistent lymphocytosis and in some cases lymphoid tumors in domestic cattle. The BLV envelope (Env) glycoprotein, which consists of the gp51 outer membrane glycoprotein and the gp30 transmembrane glycoprotein, is directly involved in infectivity events and, like the p24 major structural protein, can elicit a strong immune response in infected cattle (23). Serological diagnosis of EBL is mainly based on screening of field samples for gp51 antibodies. It follows that diagnostic procedures that use native gp51 constitute a prerequisite for the design of an efficient EBL eradication program. In recent decades different serological methods, such as the agar gel immunodiffusion test (AGID) and the enzyme-linked immunosorbent assay (ELISA), have been developed. ELISAs are based on the use of partially purified BLV gp51 and monoclonal antibodies (MAbs) against BLV gp51 epitopes (4, 17). These procedures are particularly useful for samples with low antibody titers, such as milk samples or pooled sera. A permanent fetal lamb kidney (FLK) cell line chronically infected with BLV (FLK-BLV) is used to produce gp51. Although highly productive BLV-infected cell lines are available, their production is rather laborious, expensive, and time-consuming.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in Sandwich ELISA, Lateral flow immunoassay (LFIA), and other immunoassays;
Formulation	Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No.	GMP-AD-Ruminants-14Ag
Antigens	Bovine viral diarrhea virus (BVDV) gp55
Resource (expression host)	E.coli
Specics/Isotypes	Bovine viral diarrhea virus (BVDV) gp55
Bioactivity validation	Antibody Binding, Immunogen in Sandwich Elisa, lateral-flow tests,and other immunoassays in Bovine viral diarrhea virus (BVDV) gp55 level test and Ruminants-diagnositcs.
Tag	His
Antigen description	Bovine leukemia virus (BLV), the etiological agent of enzootic bovine leukosis (EBL), is a C-type retrovirus of cattle with a worldwide distribution (5). The virus induces a persistent lymphocytosis and in some cases lymphoid tumors in domestic cattle. The BLV envelope (Env) glycoprotein, which consists of the gp51 outer membrane glycoprotein and the gp30 transmembrane glycoprotein, is directly involved in infectivity events and, like the p24 major structural protein, can elicit a strong immune response in infected cattle (23). Serological diagnosis of EBL is mainly based on screening of field samples for gp51 antibodies. It follows that diagnostic procedures that use native gp51 constitute a prerequisite for the design of an efficient EBL eradication program. In recent decades different serological methods, such as the agar gel immunodiffusion test (AGID) and the enzyme-linked immunosorbent assay (ELISA), have been developed. ELISAs are based on the use of partially purified BLV gp51 and monoclonal antibodies (MAbs) against BLV gp51 epitopes (4, 17). These procedures are particularly useful for samples with low antibody titers, such as milk samples or pooled sera. A permanent fetal lamb kidney (FLK) cell line chronically infected with BLV (FLK-BLV) is used to produce gp51. Although highly productive BLV-infected cell lines are available, their production is rather laborious, expensive, and time-consuming.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in Sandwich ELISA, Lateral flow immunoassay (LFIA), and other immunoassays;
Formulation	Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage	Store at -20℃ to -163℃ under sterile conditions. Avoid repeated freeze-thaw cycles.