2019 nCoV (SARS CoV2 coronavirus) Ad-Spike(S1+S2)RBD triple mutation of 501Y.V2 lineage(B.1.351)(K417N, E484K and N501Y) Pre-made adenovirus vector

Order information

Catalog No.	Package	Price(In USD)	Qty (Quantity)	Sum(In USD)
GMV-V-2019nCoV-110	In vitro grade: 1E+10PFU (1E+10pfu/ml×1ml)	3390
GMV-V-2019nCoV-110	In vitro grade: 5E+10PFU (1E+10pfu/ml×5ml)	4990
GMV-V-2019nCoV-110	In vitro grade: 1E+11PFU (1E+10pfu/ml×10ml)	9090
GMV-V-2019nCoV-110	Large size	Inquiry.
GMV-V-2019nCoV-110	In vivo grade: 1E+11PFU (1E+11pfu/ml×1ml)	Inquiry.
			Shipping Cost:	760.00
			Total:

Description

2019 nCoV related Gene	Spike(S1+S2)RBD triple mutation of 501Y.V2 lineage(B.1.351)(K417N, E484K and N501Y)
Species Host	SARS-COV-2 501Y.V2 lineage(B.1.351)
Vector	Pre-made adenovirus
Reporter	null
Antibiotic in Mammalian cell	null
Length	3822
Promoter	CMV
Tag	C-3FLAG
Codon Optimized	Codon Optimized for mamamlian
Gene Accession Number	QHD43416.1
Application	Adenovirus-based 2019nCoV vaccine; Adenovirus production; Mammalian cells expression

Spike mutant variant of SARS-COV-2 (2019nCOV) 501Y.V2 lineage(B.1.351) spread in South Africa

Background Reading: Information and products collection of SARS-COV-2 (2019nCOV) South Africa 501Y.V2 lineage

The world is in midst of the COVID-19 pandemic. Recently a new SARS-CoV-2 (2019nCOV)lineage (501Y.V2), characterised by eight lineage-defining mutations in the spike protein (excluding D614G mutation), including three at important residues in the receptor-binding domain (K417N, E484K and N501Y), represents increased transmissibility. This lineage emerged in South Africa and spread rapidly, becoming within weeks the dominant lineage in the Eastern Cape and Western Cape Provinces of South Africa.

Mutations of Spike protein in SARS-COV-2 (2019nCOV) 501Y.V2 lineage(B.1.351) spread in South Africa

Three of the spike mutations are at key residues in the RBD (N501Y, E484K and K417N), three are in the N-terminal domain (L18F, D80A and D215G) and one is in loop 2 (A701V). Deletion of three amino acids at L242-244L is disputed with a L242H mutation¹.
Figure. Amino acid changes in the spike region of the 190 S501Y.V2 genomes¹

GeneMedi codon-optimized spike mammalian expression vector for SARS-COV-2 (2019nCOV) S501Y.V2 lineage(B.1.351)

GeneMedi pseudotype virus (pseudovirus) of SARS-COV-2 (2019nCOV) 501Y.V2 lineage(B.1.351)

Reference

1 Houriiyah Tegally, E. W., Marta Giovanetti, Arash Iranzadeh, Vagner Fonseca, Jennifer Giandhari, Deelan Doolabh, Sureshnee Pillay, Emmanuel James San, Nokukhanya Msomi, Koleka Mlisana, Anne von Gottberg, Sibongile Walaza, Mushal Allam, Arshad Ismail, Thabo Mohale, Allison J Glass, Susan Engelbrecht, Gert Van Zyl, Wolfgang Preiser, Francesco Petruccione, Alex Sigal, Diana Hardie, Gert Marais, Marvin Hsiao, Stephen Korsman, Mary-Ann Davies, Lynn Tyers, Innocent Mudau, Denis York, Caroline Maslo, Dominique Goedhals, Shareef Abrahams, Oluwakemi Laguda-Akingba, Arghavan Alisoltani-Dehkordi, Adam Godzik, Constantinos Kurt Wibmer, Bryan Trevor Sewell, José Lourenço, Luiz Carlos Junior Alcantara, Sergei L Kosakovsky Pond, Steven Weaver, Darren Martin, Richard J Lessells, Jinal N Bhiman, Carolyn Williamson, View ORCID ProfileTulio de Oliveira. Emergence and rapid spread of a new severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) lineage with multiple spike mutations in South Africa. medRxiv preprint, doi:https://doi.org/10.1101/2020.12.21.20248640 (2020).