Recombinant 2019nCoV antigen-coronavirus papain-like proteinase (PLpro, Nsp3, His Tag)

Order information

Package	Catalog No.	Price(In USD)	Qty (Quantity)	Sum(In USD)
1mg	GMP-V-2019nCoV-PLpro001-1mg	5990
10mg	GMP-V-2019nCoV-PLpro001-10mg	38790
100mg	GMP-V-2019nCoV-PLpro001-100mg	258000
≥100mg	GMP-V-2019nCoV-PLpro001-xmg	Inquiry
			Shipping Cost:	760.00
			Total:

Description

Accession Number	QHD43415.1
Expression platform	2019-nCoV(SARS-CoV-2, SARS2-coronavirus)
Isotypes	E.coli
Tag	N-His
Products description	Recombinant 2019-nCoV(SARS-CoV-2, SARS2-coronavirus) papain-like Proteinase (PLpro, PL Proteinase,Nsp3) was expressed in E.coli-based prokaryotic cell expression system and the target gene encoding Glu1564-IIe1877 was expressed with 6 HIS tag at the N-terminus.
Bioactivity validation	In Testing
Purity	Purity: ≥95% (SDS-PAGE)
application	Immunogen in Elisa,lateral-flow tests,and other immunoassays;Standard substance The antigen can also be used in drug discovery including antibody screening and lead compound candidates assay.
predicted Molecular Mass	38.6kD
Formulation & Reconstitution	Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

2019nCoV (SARS-CoV-2) Papain-Like Proteinase (PLpro)

About Coronavirus Papain-Like Proteinase (PLpro)

COVID-19 pandemic is caused by 2019nCoV (SARS-CoV-2) infection. 2019nCoV contains 16 Non-structure protein (Nsp1-Nsp16) that may be drugable targets for antiviral compounds discovery against COVID-19¹.

2019nCoV (SARS-CoV-2) Papain-Like Proteinase(PLpro) is included in Nsp3. The coronavirus Papain-Like Proteinase(PLpro) has been found as a nucleic acid-binding domain (NAB) with a nucleic acid chaperon function, which is conserved in coronavirus，and one uncharacterized domain termed the marker domain (G2M). Following the G2M are two predicted double-pass transmembrane domains (TM1–2 and TM3–4), a putative metal binding region (ZN) and the Y domain of unknown function (subdomains Y1–3)^1,2.

About Nsp3

Nsp3: Nsp3 (200 kDa) is the largest protein encoded by the coronavirus genome (SARS-CoV-2, SARS-CoV, MERS, etc.). Nsp3 is an essential component of the replication and transcription complex. It comprises various domains, the organization of which differs between coronavirus genera, due to duplication or absence of some domains. However, the N-terminal region of the Nsp3 is highly conserved among coronavirus, containing a ubiquitin-like (Ubl) globular fold followed by a flexible, extended acidic-domain (AC domain) rich in glutamic acid (38%). Next to the AC domain is a catalytically active ADP-ribose-1"-phosphatase (ADRP, app-1"-pase) domain (also called macro domain or X domain) thought to play a role during synthesis of viral subgenomic RNAs. SARS Unique Domain (SUD), a domain not yet identified in other coronaviruses from alphacoronavirus and betacoronavirus, follows next. The SUD domain binds oligonucleotides known to form G-quadruplexes. Downstream of the SUD domain is a second Ubl domain and the catalytically active PLpro domain that proteolytically processes the Nsp1/2, Nsp2/3 and Nsp3/4 cleavage sites^1,2

High-throughput screening (HTS) assay development of Coronavirus PLpro for antiviral compounds

HTS Approach: Fluorescence resonance energy transfer (FRET) assay for time-dependent kinetic analysis
FRET Substrate: (E-EDANS)RELNGGAPI(K-DABCYL)S (Synthetic)

Simple 2019nCoV (SARS-CoV-2) Papain-Like Proteinase(PLpro) activity assay develoement Protocol²: 2019nCoV (SARS-CoV-2) Papain-Like Proteinase(PLpro) activity assay develoement Protocol article


References

Recombinant 2019 nCoV (SARS2 coronavirus) Antigens reagents

Cat No.	Antigen Name of 2019-nCoV(SARS-CoV-2)	Source (Expression Host)	Tag	Bioactivity validation	Order
GMP-V-2019nCoV-N002	Recombinant 2019-nCoV(SARS-CoV-2) Nucleocapsid Protein (N protein,N-His tag)	E.coli	N-His
GMP-V-2019nCoV-SRBD001	Recombinant 2019-nCoV(SARS-CoV-2) Spike Protein (RBD, C-His Tag)	mammalian (human cell)	C-His	ACE2 binding;
GMP-V-2019nCoV-SRBD002	Recombinant 2019-nCoV(SARS-CoV-2) Spike Protein (RBD, C-mFC Tag)	mammalian (human cell)	C-mFc	ACE2 binding;
GMP-V-2019nCoV-S1001	Recombinant 2019-nCoV(SARS-CoV-2) Spike Protein (S1, His Tag)	Baculovirus-Insect Cells	C-His
GMP-V-2019nCoV-S1S2001	Recombinant 2019-nCoV(SARS-CoV-2) Spike Protein (S1+S2 ECD, His tag)	Baculovirus-Insect Cells	C-His
GMP-V-2019nCoV-E001	Recombinant 2019-nCoV(SARS-CoV-2) Envelope Protein (E protein,His Tag)	E.coli	N-His
GMP-H-ACE2001	Recombinant human soluble ACE2 protein (soluble hACE2,extracellular hACE2,C-His)	mammalian (human cell)	C-His	Spike(S-RBD) protein binding
GMP-H-ACE2002	Recombinant human soluble ACE2 protein (soluble hACE2, extracellular hACE2, C-FC)	mammalian (human cell)	C-Fc	Spike(S-RBD) protein binding
GMP-V-2019nCoV-Mpro001	Recombinant 2019-nCoV(SARS-CoV-2) Main Proteinase (Mpro, 3CLpro, His Tag)	E.coli	N-His	Enzyme activity validation in biochemical assay
GMP-V-2019nCoV-PLpro001	Recombinant 2019-nCoV(SARS-CoV-2) papain-like proteinase (PLpro, Nsp3, His Tag)	E.coli	N-His	In Testing
GMP-V-2019nCoV-RdRP001	Recombinant 2019-nCoV(SARS-CoV-2) RNA-directed RNA polymerase(RdRP, Nsp12, His Tag)	E.coli	C-His	In Testing
GMP-V-2019nCoV-Nsp3X-01	Recombinant 2019-nCoV(SARS-CoV-2) Nsp3-X domain(Macro domain, His tag)	E.coli	N-His	In Testing
GMP-V-2019nCoV-Nsp10-01	Recombinant 2019-nCoV(SARS-CoV-2) nsp10 (nsp10-CysHis,GFL protein,His Tag)	E.coli	N-His	In Testing
GMP-V-2019nCoV-Nsp16-01	Recombinant 2019-nCoV(SARS-CoV-2) nsp16 (nsp16-OMT,2'-o-MT,His Tag)	E.coli	N-His	In Testing

2019 nCoV (SARS2 coronavirus) Antibodies for COVID-19

Cat No.	Products Name	Expression platform	Isotypes	Bioactivity validation	Order
GMP-V-2019nCoV-NAb001	Anti-2019-nCoV NP human monoclonal antibody	human IgG	Mammalian (human cell)	N protein binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with GMP-V-2019nCoV-NAb002 , GMP-V-2019nCoV-NAb003 , GMP-V-2019nCoV-NAb004.
GMP-V-2019nCoV-NAb002	Anti-2019-nCoV NP human scFv-Fc antibody	Scfv	Mammalian (human cell)	N protein binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with GMP-V-2019nCoV-NAb001, GMP-V-2019nCoV-NAb003 , GMP-V-2019nCoV-NAb004.
GMP-V-2019nCoV-NAb003	Anti-2019-nCoV NP mouse monoclonal antibody (mAb)	Mouse IgG	Hybridoma	N protein binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with GMP-V-2019nCoV-NAb001, GMP-V-2019nCoV-NAb002, GMP-V-2019nCoV-NAb004.
GMP-V-2019nCoV-NAb004	Anti-2019-nCoV NP mouse monoclonal antibody (mAb)	Mouse IgG	Hybridoma	N protein binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with GMP-V-2019nCoV-NAb001, GMP-V-2019nCoV-NAb002 , GMP-V-2019nCoV-NAb003.
GMP-V-2019nCoV-S1Ab001	Anti-2019-nCoV Spike (S1 protein) human monoclonal antibody	human IgG	Mammalian (human cell)	S-RBD protein binding, ELISA validated
GMP-V-2019nCoV-S1Ab002	Anti-2019-nCoV Spike (S1 protein) human monoclonal antibody	human IgG	Mammalian (human cell)	S-RBD protein binding, ELISA validated, Western Blot validated

GeneMedi also provides pre-made the lentivirus, adenovirus and AAV vector for the gene ORF plasmids of 2019 nCoV (SARS2 coronavirus):
https://www.genemedi.net/i/2019-ncov-gene-vectors