Recombinant 2019nCoV antigen-coronavirus Nsp3-X domain(Macro domain, His tag)

Order information

Package	Catalog No.	Price(In USD)	Qty (Quantity)	Sum(In USD)
1mg	GMP-V-2019nCoV-Nsp3X-01-1mg	5990
10mg	GMP-V-2019nCoV-Nsp3X-01-10mg	38790
100mg	GMP-V-2019nCoV-Nsp3X-01-100mg	258000
≥100mg	GMP-V-2019nCoV-Nsp3X-01-xmg	Inquiry
			Shipping Cost:	760.00
			Total:

Description

Accession Number	QHD43415.1
Expression platform	2019-nCoV(SARS-CoV-2, SARS2-coronavirus)
Isotypes	E.coli
Tag	N-His
Products description	Recombinant 2019-nCoV(SARS-CoV-2)Nsp3-X domain(Macro domain) was expressed in E.coli-based prokaryotic cell expression system and the target gene encoding Glu1024-Gln1198 was expressed with 6 HIS tag at the N-terminus. The Macro domain is a module of ~180 amino acids which can bind ADP-ribose, an NAD metabolite or related ligands.
Bioactivity validation	In Testing
Purity	Purity: ≥85% (SDS-PAGE)
Application	Immunogen in Elisa,lateral-flow tests,and other immunoassays;Standard substance The antigen can also be used in drug discovery including antibody screening and lead compound candidates assay.
predicted Molecular Mass	22kD
Formulation & Reconstitution	Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

About Nsp3

COVID-19 pandemic is caused by 2019nCoV (SARS-CoV-2) infection. 2019nCoV contains 16 Non-structure protein (Nsp1-Nsp16) that may be drugable targets for antiviral compounds discovery against COVID-19¹.

Nsp3: Nsp3 (200 kDa) is the largest protein encoded by the coronavirus genome (SARS-CoV-2, SARS-CoV, MERS, etc.). Nsp3 is an essential component of the replication and transcription complex. It comprises various domains, the organization of which differs between coronavirus genera, due to duplication or absence of some domains. However, the N-terminal region of the Nsp3 is highly conserved among coronavirus, containing a ubiquitin-like (Ubl) globular fold followed by a flexible, extended acidic-domain (AC domain) rich in glutamic acid (38%). Next to the AC domain is a catalytically active ADP-ribose-1"-phosphatase (ADRP, app-1"-pase) domain (also called macro domain or X domain) thought to play a role during synthesis of viral subgenomic RNAs. SARS Unique Domain (SUD), a domain not yet identified in other coronaviruses from alphacoronavirus and betacoronavirus, follows next. The SUD domain binds oligonucleotides known to form G-quadruplexes. Downstream of the SUD domain is a second Ubl domain and the catalytically active PLpro domain that proteolytically processes the Nsp1/2, Nsp2/3 and Nsp3/4 cleavage sites^1,2.

References