Recombinant 2019nCoV antigen-coronavirus Main Proteinase
(Mpro, 3CLpro, His Tag)

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Cat No.: GMP-V-2019nCoV-Mpro001

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Package Catalog No. Price(In USD) Qty (Quantity) Sum(In USD)
1mg

GMP-V-2019nCoV-Mpro001-1mg

 5990
10mg

GMP-V-2019nCoV-Mpro001-10mg

 38790
100mg

GMP-V-2019nCoV-Mpro001-100mg

 258000
≥100mg

GMP-V-2019nCoV-Mpro001-xmg

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Description

Accession Number YP_009725301.1
Expression platform 2019-nCoV(SARS-CoV-2, SARS2-coronavirus)
IsotypesE.coli
TagN-His
Products description Recombinant 2019-nCoV(SARS-CoV-2, SARS2-coronavirus) Main Proteinase (Mpro, 3CLpro, His Tag)
was expressed in E.coli-based prokaryotic cell expression system and the target gene encoding Ser1-Gln306
was expressed with 6 HIS tag at the N-terminus.
Bioactivity validationEnzyme activity validation in biochemical assay
PurityPurity: ≥95%(SDS-PAGE)
ApplicationImmunogen in Elisa,lateral-flow tests,and other immunoassays; Standard substance.
The antigen can also be used in drug discovery including antibody screening and lead compound candidates assay.
predicted Molecular Mass35kD
Formulation & ReconstitutionLyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4);
For PSB2, reconstituted with 0.9% sodium chloride;
For PBS, reconstituted with ddH2O.
StorageStore at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

2019nCoV (SARS-CoV-2) Mpro (3CLpro, 3C-Like protein, Nsp5)


About Coronavirus Mpro (Coronavirus 3CLpro, 3C-Like protein)

COVID-19 pandemic is caused by 2019nCoV (SARS-CoV-2) infection. 2019nCoV contains 16 Non-structure protein (Nsp1-Nsp16) that may be drugable targets for antiviral compounds discovery against COVID-191.

2019nCoV (SARS-CoV-2) Mpro (Main Protease,Nsp5), also called 3C-Like Protein (3CLpro). The 2019nCoV (SARS-CoV-2) Mpro (3CLpro) mediates processing at 11 distinct cleavage sites, including its own autoproteolysis, and is essential for coronavirus replication. The coronavirus Mpro (3CLpro) exhibits a conserved three-domain structure and catalytic residues1,2.

High-throughput screening (HTS) assay development of coronavirus Mpro (3CLpro) for antiviral compounds

HTS Approach: Fluorescence resonance energy transfer (FRET) assay for time-dependent kinetic analysis

1.FRET Substrate: MCA-AVLQSGFR-Lys(Dnp)-Lys-NH22
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2. Simple 2019nCoV (SARS-CoV-2) Mpro (3C-Likepro) activity assay develoement Protocol2: 2019nCoV (SARS-CoV-2) Mpro (3C-Likepro) activity assay develoement Protocol article pdf download

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References

1. Gordon, D. E. et al. A SARS-CoV-2-Human Protein-Protein Interaction Map Reveals Drug Targets and Potential Drug-Repurposing. BioRxiv, doi:10.1101/2020.03.22.002386 (2020).
2. Jin, Z. et al. Structure of M(pro) from COVID-19 virus and discovery of its inhibitors. Nature, doi:10.1038/s41586-020-2223-y (2020).