Tissue-specific Cre mouse + AAV-DIO-GOI for tissue-specific gene overexpression
Product list: Cre/loxP tools in AAV vector(AAV-Cre), adenoviral vector(Ad-Cre) and lentiviral vector(Lv-Cre)
| Cat No. | Pre-made Cre | Viral vector | Protomer | Promoter Tissue Specificity |
Promoter Tissue Specificity characteristics |
| GMV-AdCre-01 | Ad-CMV-Cre | Adenovirus | CMV | Total | Total |
| GMV-AdCre-02 | Ad-CMV-Cre-GFP | CMV | Total | Total | |
| GMV-AAV9Cre-01 | AAV9-CMV-Cre | AAV GeneMedi offer multiple serotypes of AAV-Cre loxP system for your option:
· AAV1-Cre · AAV2-Cre · AAV2 variant (Y444F)-Cre · AAV2 variant (Y272F, Y444F, Y500F, Y730F)-Cre · AAV2 variant (Y444F, Y730F, Y500F, Y272F, Y704F, Y252F)-Cre · AAV2 variant(AAV2.7m8)-Cre · AAV5-Cre · AAV6-Cre · AAV8-Cre · AAV8-1m-Cre · AAV8-2m-Cre · AAV8 variant (Y733F, Y447F, Y275)-Cre · AAV9-Cre · AAV-Rh.10-Cre · AAV-DJ-Cre · AAV-DJ/8-Cre · AAV-Retro (Retrograde)-Cre · AAV-PHP.B-Cre · AAV9-PHP.A-Cre · AAV-PHP.eB-Cre · AAV-PHP.S-Cre | CMV | Total | Total |
| GMV-AAV9Cre-02 | AAV9-CMV-Cre-ZsGreen | CMV | Total | Total | |
| GMV-AAV9Cre-03 | AAV9-CAG-Cre | CAG | Total | Total | |
| GMV-AAV9Cre-04 | AAV9-CAG-Cre-ZsGreen | CAG | Total | Total | |
| GMV-AAV9Cre-05 | AAV9-Syn-Cre-EGFP | synapsin (SYN) | Neuro | Neuron-specific promoter | |
| GMV-AAV9Cre-06 | AAV9-Syn-Cre | synapsin (SYN) | Neuro | Neuron-specific promoter | |
| GMV-AAV9Cre-07 | AAV9-CaMKII-Cre-EGFP | CaMKII | Neuro | Forebrain glutamate neuron-specific promoter | |
| GMV-AAV9Cre-08 | AAV9-CaMKII-Cre | CaMKII | Neuro | Forebrain glutamate neuron-specific promoter | |
| GMV-AAV9Cre-09 | AAV9-GFAP-Cre-EGFP | GFAP | Neuro | Astrocyte specific promoter | |
| GMV-AAV9Cre-10 | AAV9-GFAP-Cre | GFAP | Neuro | Astrocyte specific promoter | |
| GMV-AAV9Cre-11 | AAV9-cTNT-Cre | cTnT | heart | Cardiomyocyte specific promoter | |
| GMV-AAV9Cre-12 | AAV9-cTNT-Cre-EGFP | cTnT | heart | Cardiomyocyte specific promoter | |
| GMV-AAV9Cre-13 | AAV9-TBG-Cre | TBG | liver | Hepatic specific promoter | |
| GMV-AAV9Cre-14 | AAV9-TBG-Cre-ZsGreen | TBG | liver | Hepatic specific promoter | |
| GMV-AAV9Cre-15 | AAV9-TIE-Cre | TIE | endothelial | Endothelial-specific promoter | |
| GMV-AAV9Cre-16 | AAV9-cd68-Cre | cd68 | Neuro | Microglia-specific promoter | |
| GMV-AAV9Cre-17 | AAV9-c-fos-Cre | c-fos | Neuro | Excitatory neuron promoter | |
| GMV-AAV9Cre-18 | AAV9-mecp2-Cre | mecp2 | Neuro | Shorter neuron-specific promoter | |
| GMV-AAV9Cre-19 | AAV9-MHCK7-Cre | MHCK7 | muscle | Muscle specific promoter | |
| GMV-AAV9Cre-20 | AAV9-Rpe65-Cre | Rpe65 | retina | Retinal specific promoter | |
| GMV-AAV9Cre-21 | AAV9-sm22a-Cre | sm22a | muscle | Smooth muscle specific promoter | |
| GMV-AAV9Cre-22 | AAV9-Spc5-12-Cre | Spc5-12 | muscle | Myocyte specific promoter | |
| GMV-AAV9Cre-23 | AAV9-Ta1-Cre | Tal | Neuro | Early neuron-specific promoter | |
| GMV-LvCre-01 | Lv-CMV-Cre | Lentivirus | CMV | Total | Total |
| GMV-LvCre-02 | Lv-CMV-Cre-zsgreen | CMV | Total | Total |
Abstract
Cre-loxP system is widely used in the field of biosciences, especially in the generation of genetically engineered mice (knockout or overexpression), enabling researchers to study the function of gene of interest (GOI). To date, numerous systems, derived from the basic Cre-loxP system, have been developed to precisely control gene expression at desired time or cells. Here, we briefly introduce Cre-loxP system, and give a summary about its applications, especially viral vectors-mediated Cre expression (such as AAV-Cre or Ad-Cre) for in vitro or in vivo studies.Tissue-specific Cre mouse + AAV-DIO-GOI for tissue-specific gene overexpression
To facilitate the use of Cre-DIO system, AAV vectors have been introduced to tissue-specific Cre mouse to mediate tissue-specific gene overexpression. Without injection of AAV vector carrying DOI-GOI system (AAV-DIO-GOI), tissue X-specific Cre mouse cannot express GOI and reporter gene in tissue X. After injection of AAV-DIO-GOI into tissue X, Cre recombinase will interact with lox sites, thus activating the expression of GOI and reporter. Synchronously, the tissue X will be labeled by the reporter gene (Fig. 7). This system utilizes high cell-specific promoter to drive Cre recombinase expression and a strong promoter to drive GOI expression, which ideally occur just at the appropriate time point and specific cells. As a powerful system with high spatiotemporal fidelity, AAV-DIO exhibits great advantages and potential in the study of neural circuits and networks, behavior, specific cell populations (lineage tracing), animal models of disease, as well as in high-throughput ex vivo researches.
Summary
The Cre-loxP system, especially inducible tissue-specific knockout and viral vector-mediated Cre expression, has been highly utilized in genetics and cell biology research. Nevertheless, it seems that Cre-loxP system will continue to be prevalent in current and future research studies. GeneMedi is proficient in viral vector development and offers kinds of viral vector-based Cre tools, we can provide the best services and products if required.References
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