AAV9 vector system (AAV9 expression system, AAV9 packaging plasmid system) (Amp and Kanamycin) (GeneMedi Owned)

GeneMedi (GM) AAV9 Vector system is including: 1) AAV9 Rep-Cap plasmid (AAV9-RC plasmid) 2) AAV Helper plasmid 3) AAV expression vectors (overexpression or shRNA).

You can produce AAV9 particle in 293T cell line in high titer using GeneMedi's AAV9 Rep-Cap plasmid with GM's AAV expression vector (overexpression or shRNA) and AAV helper plasmid.

The tissue tropism of AAV6 vector has been validated in neuron(CNS),lung,liver,heart and muscle,with potential applications in tissue-specific gene therapy.AAV6 has also been proved a high transduction efficency in T cell,wtih potential applications in T cell-mediated cancer immunotherapies, including Chimaeric antigen receptor T(CAR-T) and TCR engineered T(TCR-T) cell therapy.

GeneMedi offers both Ampicillin (Amp) resistance and Kanamycin (Kan) resistance for the AAV Rep-Cap Plasmids, AAV expression Plasmids and AAV helper Plasmid.

Target products collectionGo to AAV vector system >>

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Interleukin 6 (IL-6)

Product Description

Cat.NO Product Name Organization Type Price(In USD)
P-PK08 AAV9 Vector System (Amp resistance)
1. AAV9 Rep-Cap Plasmid, 5ug
2. AAV Helper Plasmid, 5ug
3. pGMAAV-CMV-MCS-T2A-ZsGreen (P-AAVV-B01), 5ug
Academic 2028
P-PK08 AAV9 Vector System (Amp resistance)
1. AAV9 Rep-Cap Plasmid, 5ug
2. AAV Helper Plasmid, 5ug
3. pGMAAV-CMV-MCS-T2A-ZsGreen (P-AAVV-B01), 5ug
Industry R&D
(excluding
CRO&CDMO&CXO)
P-PK08 AAV9 Vector System (Amp resistance)
1. AAV9 Rep-Cap Plasmid, 5ug
2. AAV Helper Plasmid, 5ug
3. pGMAAV-CMV-MCS-T2A-ZsGreen (P-AAVV-B01), 5ug
CRO&CDMO&
CXO&CMC&
Manufacturing company
P-PK08 AAV9 Vector System (Amp resistance)
1. AAV9 Rep-Cap Plasmid, ≥10mg
2. AAV Helper Plasmid, ≥10mg
3. pGMAAV-CMV-MCS-T2A-ZsGreen (P-AAVV-B01-Kan), ≥10mg
 All
P-PK08-Kan AAV9 Vector System (Kan resistance)
1. AAV9 Rep-Cap Plasmid (Kan resistance) , ≥10mg
2. AAV Helper Plasmid (Kan resistance) , ≥10mg
3. pGMAAV-CMV-MCS-T2A-ZsGreen (Kan resistance) (P-AAVV-B01-Kan), ≥10mg
 All
P-RC08 AAV9 Rep-Cap Plasmid (Amp resistance)
Size：5ug 		Academic 935
P-RC08 AAV9 Rep-Cap Plasmid (Amp resistance)
Size：5ug 		Industry R&D
(excluding
CRO&CDMO&CXO)
P-RC08 AAV9 Rep-Cap Plasmid (Amp resistance)
Size：5ug 		CRO&CDMO&
CXO&CMC&
Manufacturing company
P-RC08 AAV9 Rep-Cap Plasmid (Amp resistance)
Size：≥10mg 		All
P-RC08-Kan AAV9 Rep-Cap Plasmid(Kan resistance)
Size：≥10mg 		All
GeneMedi offers both seed and amplification services for the Amp-resistant AAV Vector System.
GeneMedi only offers amplification services and do not provide seed for the Kan-resistant AAV Vector System currently.
If you want to obtain higher quantities of plasmids (mg), please visit GM TransExcellent Plasmid DNA Rapid Preparation Service.
Terms of sale:
For Seed (5ug):
The purchaser may not reverse engineer this product to extract the sequence for independent use. The purchaser may not transfer this product to others for manufacturing purposes. The purchaser may not use this material to manufacture this product for any other party.

For plasmid production& amplification (≥10mg):
The purchaser may not reverse engineer this product to extract the sequence for independent use. The purchaser may not transfer this product to others for manufacturing purposes. The purchaser may not use this material to manufacture this product for themselves or any other party.

Citation

Title Publication Date
LINE-1 promotes tumorigenicity and exacerbates tumor progression via stimulating metabolism reprogramming in non-small cell lung cancer Molecular Cancer 2022/7/16
N6-methyladenosine-modified TRAF1 promotes sunitinib resistance by regulating apoptosis and angiogenesis in a METTL14-dependent manner in renal cell carcinoma Molecular Cancer 2022/5/10
Mitochondrial fission drives neuronal metabolic burden to promote stress susceptibility in male mice Nature Metabolism 2023/11/20
Reduced hepatic bradykinin degradation accounts for cold-induced BAT thermogenesis and WAT browning in male mice Nature Communications 2023/5/2
Ferritinophagy Mediated by Oxidative Stress-Driven Mitochondrial Damage Is Involved in the Polystyrene Nanoparticles-Induced Ferroptosis of Lung Injury ACS nano 2023/12/12
Endothelial response to type I interferon contributes to vasculopathy and fibrosis and predicts disease progression of systemic sclerosis. Arthritis & Rheumatology 2023/7/24
HuR regulates telomerase activity through TERC methylation NATURE COMMUNICATIONS 2018/6/7
Splicing factor YBX1 regulates bone marrow stromal cell fate during aging The EMBO Journal 2023/3/21
Hexavalent chromium induces γH2AX and RAD51 involved in DNA damage repair in BEAS-2B cells by modulating LNC-DHFR-4: 1 Environment International 2023/3/21
Nanoprodrug ratiometrically integrating autophagy inhibitor and genotoxic agent for treatment of triple-negative breast cancer Biomaterials 2022/3/10


Validation data

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Figure. Selected transduction results of AAV9 in mouse liver.

Virus and titer: AAV9-GFP, AAV9-pTBG-Luciferase (AAV-Luc), 1.4×1012 vg/ml
Animal: mouse, C57, 2 months
Gene delivery method: tail vein, 100μl
Determine assay: 3 weeks post infection, frozen section, immunofluorescence microscopy, in vivo imaging
Conclusion: intravenous injection of AAV9-pTBG-Luciferase (AAV-Luc) only infects liver cells and no non-specific infection

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Figure. Selected transduction results of AAV9-GFP in rat heart (A, B, C) and mouse heart (D).

Virus and titer: AAV9-GFP, 1×1012 vg/ml
Animal: Rat, SD, 2 months (Figure 8A, B, C); mouse, C57, 8 months (Figure 8D)
Infection site: heart
Gene delivery method: myocardial in situ injection, 10μl/site, 5 sites in total (Figure 8A, B, C);intraorbital intravenous injection (Figure 8D)
Determine assay: 3 weeks post infection, frozen section, immunofluorescence microscopy

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Figure. Selected transduction results of AAV9-GFP in mouse brain.

Virus and titer: AAV9-GFP, 1×1012 vg/ml
Animal: Mouse, C57, 2 months
Infection site: Brain
Gene delivery method: Brain localization injection, 1μl
Determine assay: 3 weeks post infection, frozen section, immunofluorescence microscopy

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Figure. Selected transduction results of AAV in vitro (in 293T cell line)

Virus and titer: AAV1/AAV6/AAV8/AAV-Rh10/AAV-DJ/AAV9-GFP, 1×1012 vg/ml
Cells: HEK-293T
MOI: MOI=1×104
Determine assay: 36 hours post infection, immunofluorescence microscopy

transduction results of AAV2 (left) /AAV-DJ (middle) / AAV9 (right) in tumor
Figure. Selected transduction results of AAV2 (left) /AAV-DJ (middle) / AAV9 (right) in tumor.

Virus and titer: AAV2 (left) /AAV-DJ (middle) / AAV9 (right), 1×1012 vg/ml
Animal: Nude mouse, 2 months
Infection site: Subcutaneous transplant of bowel cancer cells
Gene delivery method: Tumor injection, 10μl/site, 4 sites in total
Determine assay: 3 weeks post infection, frozen section, immunofluorescence microscopy

transduction results of AAV9-GFP in mouse hippocampus
Figure. Selected transduction results of AAV9-GFP in mouse hippocampus.

Virus and titer: AAV9-GFAP-GFP, 1×1012 vg/ml
Animal: mouse, C57, 2 months
Infection site: Hippocampus
Gene delivery method: Brain localization injection, 1μl
Determine assay: 3 weeks post infection, wholemount, immunofluorescence microscopy

transduction of AAV9 in mouse skeletal muscle
Figure. Selected transduction results of AAV9 in mouse skeletal muscle.

Virus and titer: AAV9-Luciferase (AAV-Luc), AAV9-GFP, 1×1012 vg/ml
Animal: Mouse, C57, 2 months
Infection site: Skeletal muscles
Gene delivery method: Muscle in situ injection, 10μl/site, 4 sites in total
Determine assay: 4 weeks post infection, in vivo imaging, frozen section, immunofluorescence microscopy

transduction of AAV9-GFP in mouse muscle
Figure. Selected transduction results of AAV9-GFP in mouse muscle.

Virus and titer: AAV-DJ-GFP, AAV9-GFP, 1×1012 vg/ml
Animal: mouse, C57, 2 months
Infection site: Kidney
Gene delivery method: Multiple sites injection in kidney 10μl/site, 6 sites in total
Determine assay: 3 weeks post infection, frozen section, immunofluorescence microscopy

transduction of AAV9-GFP in mouse fat tissue
Figure. Selected transduction results of AAV9-GFP in mouse fat tissue.

Virus and titer: AAV9-GFP, 1×1012 vg/ml
Animal: mouse, C57, 2 months
Infection site: Mammary fat pad
Gene delivery method: Breast injection, 10μl/site, 4 sites in total
Determine assay: 3 weeks post infection, frozen section, immunofluorescence microscopy


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