β-isomerized C-terminal telopeptides (β-CTx) antibody and antigen (Recombinant protein)

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Product information

Catalog No.	Description	US $ Price (per mg)
GMP-h-CTx-Ag01	Recombinant human β-CTx Protein	3090
GMP-h-CTx-Ab01	Anti-human β-CTx mouse monoclonal antibody (mAb)	1953
GMP-h-CTx-Ab02	Anti-human β-CTx mouse monoclonal antibody (mAb)	1953
GMP-h-CTx-Ab03	Anti-human β-CTx human monoclonal antibody (mAb)	1953
GMP-h-CTx-Ab04	Anti-human β-CTx human monoclonal antibody (mAb)	1953

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Product Description

Cat No. of Products	GMP-h-CTx-Ag01
Product Name	Recombinant human β-CTx Protein
Target/Biomarker	β-isomerized C-terminal telopeptides (β-CTx)
Expression platform	mammalian
Isotypes	Recombinant Antigen
Bioactivity validation	β-isomerized C-terminal telopeptides (β-CTx) antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in β-CTx level test of Metabolic diseases (osteoporosis) and related syndrome evaluation.
Tag	His
Products description	Recombinant human β-CTx Protein was expressed in mammalian expression system and is expressed with 6 HIS tag at the C-terminus.
Purity	Purity: ≥95% (SDS-PAGE)
Application	positive control, ELISA test,Lateral flow immunoassay (LFIA),colloidal gold immunochromatographic assay, Chemiluminescent immunoassay (CLIA),turbidimetric inhibition immuno assay (TINIA) and Immunonephelometry.
Formulation & Reconstitution	Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No. of Products	GMP-h-CTx-Ab01, GMP-h-CTx-Ab02
Product Name	Anti-human β-CTx mouse monoclonal antibody (mAb)
Target/Biomarker	β-isomerized C-terminal telopeptides (β-CTx)
Expression platform	CHO
Isotypes	Mouse IgG
Bioactivity validation	β-isomerized C-terminal telopeptides (β-CTx) antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in β-CTx level test of Metabolic diseases (osteoporosis) and related syndrome evaluation.
Tag	mFc
Products description	Anti-human β-CTx mouse monoclonal antibody (mAb) was expressed in CHO expression system and is expressed with 6 HIS tag at the C-terminus.
Purity	Purity: ≥95% (SDS-PAGE)
Application	ELISA test, Lateral flow immunoassay (LFIA), colloidal gold immunochromatographic assay, Chemiluminescent immunoassay (CLIA), turbidimetric inhibition immuno assay (TINIA), immunonephelometry and POCT
Formulation & Reconstitution	Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No. of Products	GMP-h-CTx-Ab03, GMP-h-CTx-Ab04
Product Name	Anti-human β-CTx human monoclonal antibody (mAb)
Target/Biomarker	β-isomerized C-terminal telopeptides (β-CTx)
Expression platform	CHO
Isotypes	Human lgG1
Bioactivity validation	β-isomerized C-terminal telopeptides (β-CTx) antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in β-CTx level test of Metabolic diseases (osteoporosis) and related syndrome evaluation.
Tag	hFc
Products description	Anti-human β-CTx human monoclonal antibody (mAb) was expressed in CHO expression system and is expressed with 6 HIS tag at the C-terminus.
Purity	Purity: ≥95% (SDS-PAGE)
Application	ELISA test, Lateral flow immunoassay (LFIA), colloidal gold immunochromatographic assay, Chemiluminescent immunoassay (CLIA), turbidimetric inhibition immuno assay (TINIA), immunonephelometry and POCT
Formulation & Reconstitution	Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Reference

Validation Data

Evidence has shown activated osteoclasts create resorption pits with low pH to dissolve the inorganic matrix and lysomal enzymes, such as TRAP and cathepsin K, effectively digest the exposed type-1 collagen releasing specific degradation products, such as the carboxyl-terminal cross-linked telopeptides of type I collagen (CTX-1). The biomarker β-CTx offers significant information on bone metabolism (bone remodeling) and is useful to predict fracture risk and to monitor the treatment efficacy. Recently, our R&D department demonstrated that our GMP-h-β-CTx-Ab pairs have a large linear range and good sensitivity against the GMP-h-β-CTx-Ag01. Below is the result of GeneMedi's GMP-h-β-CTx-Ab01/02/03/04 (Anti-human β-CTx mouse monoclonal antibody) validation with GMP-h-β-CTx-Ag01 (Recombinant human β-CTx Protein) in ELISA. We highly recommend the Ab01-Ab02 pair and Ag to you.

Figure 1. GeneMedi's GMP-h-β-CTx-Ab01/02/03/04 (Anti-human β-CTx mouse monoclonal antibody) are validated to detect the GMP-h-β-CTx-Ag01 (Recombinant human β-CTx Protein) in ELISA.

   EC50 [Ab01 (capture)-Ab02 (detect) ]= 271.3 ppb;

   EC50 [Ab01 (capture)-Ab04 (detect)]= 614.8 ppb;

   EC50 [Ab03 (capture)-Ab02 (detect) ]= 363.5 ppb;

   EC50 [Ab03 (capture)-Ab04 (detect) ]= 811.8 ppb.

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Target/Biomarker information

Bone is a metabolically active tissue, and almost 10% of the adult bone is remodeled every year. Bone remodeling comprises bone resorption and formation. During the remodeling process, the activity of osteoblasts and osteoclasts results in the release of proteins or peptides. The peptide products are either proteins released by the cells, such as bone-specific alkaline phosphatase (BAP) and osteocalcin (OC) or degradation products of cellular activity, such as urinary hydroxyproline, deoxypyridinoline, β-isomerized C-terminal telopeptides (β-CTx), total procollagen type 1 amino-terminal propeptide (tP1NP), etc [14]. The measurement of these clinical biomarkers, categorized as biochemical bone markers, in serum and urine is widely used as a rapid means to monitor cellular activity of bone [15]. β-CTx and other substances that originate during bone resorption are called bone resorption markers, while tP1NP, BAP, and OC, which are generated from bone reconstruction, are called bone formation markers.

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