Borrelia burgdorferi sensu lato(B.b.s.l., genospecies B.b. sensu stricto, B. garinii, B. afzelii) i antibody and antigen (recombinant protein)

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Diagnostic anti-Borrelia burgdorferi sensu lato(B.b.s.l., genospecies B.b. sensu stricto, B. garinii, B. afzelii) i antibodies pairs and antigen for animal health (animal Cat/Feline, Dog/Canine, Equine/Horse infectious disease Lyme disease/Borreliosis) testing in ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA and POCT

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Product information

Catalog No.	Description	US $ Price (per mg)
GMP-VT-P258-Ag01	Recombinant Borrelia burgdorferi sensu lato(B.b.s.l., genospecies B.b. sensu stricto, B. garinii, B. afzelii) i protein	$3090.00
GMP-VT-P258-Ab01	Anti-Borrelia burgdorferi sensu lato(B.b.s.l., genospecies B.b. sensu stricto, B. garinii, B. afzelii) i mouse monoclonal antibody (mAb)	$3090.00
GMP-VT-P258-Ab02	Anti-Borrelia burgdorferi sensu lato(B.b.s.l., genospecies B.b. sensu stricto, B. garinii, B. afzelii) i mouse monoclonal antibody (mAb)	$3090.00

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Product Description

Cat No.	GMP-VT-P258-Ag01
Product Name	Recombinant Borrelia burgdorferi sensu lato(B.b.s.l., genospecies B.b. sensu stricto, B. garinii, B. afzelii) i protein
Pathogen	Borrelia burgdorferi sensu lato(B.b.s.l., genospecies B.b. sensu stricto, B. garinii, B. afzelii) i
Expression platform	E.coli
Isotypes	Recombinant Antigen
Bioactivity validation	Anti-Borrelia burgdorferi sensu lato(B.b.s.l., genospecies B.b. sensu stricto, B. garinii, B. afzelii) i antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in Borrelia burgdorferi sensu lato(B.b.s.l., genospecies B.b. sensu stricto, B. garinii, B. afzelii) i level test of animal Cat/Feline, Dog/Canine, Equine/Horse infectious disease with Lyme disease/Borreliosis.
Tag	His
Product description	Recombinant Borrelia burgdorferi sensu lato(B.b.s.l., genospecies B.b. sensu stricto, B. garinii, B. afzelii) i proteinwas expressed in E.coli - based prokaryotic cell expression system and is expressed with 6 HIS tag at the C-terminus.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in Sandwich ELISA, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No.	GMP-VT-P258-Ab01,GMP-VT-P258-Ab02
Pathogen	Borrelia burgdorferi sensu lato(B.b.s.l., genospecies B.b. sensu stricto, B. garinii, B. afzelii) i
Product Name	Anti-Borrelia burgdorferi sensu lato(B.b.s.l., genospecies B.b. sensu stricto, B. garinii, B. afzelii) i mouse monoclonal antibody (mAb)
Expression platform	CHO
Isotypes	Mouse IgG
Bioactivity validation	Recombinant Borrelia burgdorferi sensu lato(B.b.s.l., genospecies B.b. sensu stricto, B. garinii, B. afzelii) i antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Borrelia burgdorferi sensu lato(B.b.s.l., genospecies B.b. sensu stricto, B. garinii, B. afzelii) i antibodies in Borrelia burgdorferi sensu lato(B.b.s.l., genospecies B.b. sensu stricto, B. garinii, B. afzelii) i level test of animal Cat/Feline, Dog/Canine, Equine/Horse infectious disease with Lyme disease/Borreliosis.
Product description	Anti-Borrelia burgdorferi sensu lato(B.b.s.l., genospecies B.b. sensu stricto, B. garinii, B. afzelii) i mouse monoclonal antibody (mAb) is a mouse monoclonal antibody produced by CHO technology. The antibody is ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Borrelia burgdorferi sensu lato(B.b.s.l., genospecies B.b. sensu stricto, B. garinii, B. afzelii) i antibodies./td>
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in Sandwich ELISA, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Reference

Validation Data

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Pathogen

Borrelia burgdorferi Sensu Lato (B.b.s.l.): An In-Depth Examination

Borrelia burgdorferi sensu lato (B.b.s.l.) represents a complex of spirochete bacteria responsible for one of the most prevalent vector-borne diseases globally: Lyme disease. Comprising several genospecies, including B.b. sensu stricto, B. garinii, and B. afzelii, B.b.s.l. presents a fascinating case study in bacterial pathogenesis, immune evasion, and the intricacies of disease diagnosis.

Pathogen Classification:

Borrelia burgdorferi sensu lato is categorized within the domain Bacteria and phylum Spirochaetes. Spirochetes are characterized by their distinctive spiral or corkscrew shape, which is essential for their motility. They are Gram-negative bacteria, implying the structure of their cell walls. These pathogens exhibit remarkable flexibility and agility, which plays a significant role in their survival and pathogenicity.

Pathogen Structure:

A deep understanding of B.b.s.l. requires an examination of its structural components, particularly those that interact with the host. The outer membrane of these bacteria is of paramount importance. Key proteins and structures include:

OspA (Outer Surface Protein A): OspA is an outer surface lipoprotein that acts as the initial point of contact between the bacterium and host cells. It plays a pivotal role in adhesion and the early stages of infection.

OspC (Outer Surface Protein C): OspC is an essential virulence factor in B.b.s.l. infections. This protein facilitates the bacterium's evasion of the host's immune system and is crucial in the establishment of infection. The genetic diversity of OspC among different Borrelia species and strains contributes to the variability in clinical manifestations seen in Lyme disease.

Flagella: Spirochetes, including Borrelia, are highly motile due to their flagella, which are located in the periplasmic space between the inner and outer membranes. These flagella enable the bacterium to move in highly viscous environments such as the extracellular matrix of host tissues, aiding in tissue invasion and dissemination.

Hosts and Diseases:

Borrelia burgdorferi sensu lato is a zoonotic pathogen, primarily infecting a range of animal species, including mammals and birds. Humans are considered accidental hosts in the natural transmission cycle of the bacterium. The primary disease attributed to B.b.s.l. infection is Lyme disease, which is caused by various Borrelia genospecies. The clinical presentation of Lyme disease can vary but typically includes an array of symptoms such as fever, fatigue, arthritis, and the characteristic erythema migrans, a skin lesion often referred to as a "bull's-eye" rash.

Diagnostic Methods:

Accurate diagnosis of Borrelia burgdorferi sensu lato infections is crucial for timely treatment. A comprehensive diagnostic approach may encompass the following methods:

Serological Tests: Enzyme immunoassays (EIAs) and Western blot assays are frequently employed to detect antibodies produced by the host in response to Borrelia infection. These tests are based on the presence of specific antibodies targeting Borrelia antigens, including proteins like OspC.

PCR (Polymerase Chain Reaction): Molecular techniques, such as PCR, enable the detection of Borrelia DNA in patient samples. Commonly targeted genes for PCR include those encoding crucial Borrelia proteins like OspA and OspC. PCR offers high specificity in confirming the presence of the pathogen.

Culturing: In certain instances, culturing the bacterium from patient samples is possible but less commonly used in clinical settings. Isolation of Borrelia requires specialized growth media and prolonged incubation periods, making it less practical than serological or molecular methods.

Microscopy: Dark-field microscopy is a valuable tool for visualizing Borrelia spirochetes in clinical samples. While it may not be suitable for definitive diagnosis, it can provide early clues regarding the presence of the pathogen.

It is essential to note that the choice of diagnostic method depends on various factors, including the stage of the disease, the available resources, and the specific guidelines of the healthcare facility. Clinical practitioners and laboratory professionals should keep current with the latest diagnostic methods and recommendations for Borrelia burgdorferi sensu lato to ensure accurate and timely diagnosis and treatment.

In conclusion, Borrelia burgdorferi sensu lato is a complex group of spirochete bacteria, intricately adapted to its vector, host, and environmental conditions. Understanding its structure, pathogenicity, and diagnostic methods is essential for the effective management and prevention of Lyme disease, a condition that continues to pose a significant public health challenge in many parts of the world.

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