Treponema antibody and antigen (recombinant protein)

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Diagnostic anti-Treponema antibodies pairs and antigen for animal health (animal Avian/Bird/Poultry infectious disease treponeme) testing in ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA and POCT

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Product information

Catalog No.	Description	US $ Price (per mg)
GMP-VT-P149-Ag01	Recombinant Treponema protein	$3090.00
GMP-VT-P149-Ab01	Anti-Treponema mouse monoclonal antibody (mAb)	$3090.00
GMP-VT-P149-Ab02	Anti-Treponema mouse monoclonal antibody (mAb)	$3090.00

Size： 1mg | 10mg | 100mg

Product Description

Cat No.	GMP-VT-P149-Ag01
Product Name	Recombinant Treponema protein
Pathogen	Treponema
Expression platform	E.coli
Isotypes	Recombinant Antigen
Bioactivity validation	Anti-Treponema antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in Treponema level test of animal Avian/Bird/Poultry infectious disease with treponeme.
Tag	His
Product description	Recombinant Treponema proteinwas expressed in E.coli - based prokaryotic cell expression system and is expressed with 6 HIS tag at the C-terminus.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in Sandwich ELISA, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No.	GMP-VT-P149-Ab01,GMP-VT-P149-Ab02
Pathogen	Treponema
Product Name	Anti-Treponema mouse monoclonal antibody (mAb)
Expression platform	CHO
Isotypes	Mouse IgG
Bioactivity validation	Recombinant Treponema antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Treponema antibodies in Treponema level test of animal Avian/Bird/Poultry infectious disease with treponeme.
Product description	Anti-Treponema mouse monoclonal antibody (mAb) is a mouse monoclonal antibody produced by CHO technology. The antibody is ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Treponema antibodies./td>
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in Sandwich ELISA, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Reference

Validation Data

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Pathogen

Introduction to Treponema:

Treponema is a genus of spirochete bacteria, a distinctive group known for their helical, corkscrew-like shape. Within this genus, one of the most medically significant species is Treponema pallidum. This pathogen is responsible for causing several serious diseases in humans, including syphilis, yaws, and bejel. In this comprehensive discussion, we will explore Treponema pallidum in greater detail, covering its classification, structure, host interactions, associated diseases, and diagnostic methods.

Classification:

Treponema pallidum belongs to the phylum Spirochaetes. This phylum comprises spiral-shaped bacteria with unique motility mechanisms. These bacteria are Gram-negative and possess an outer membrane, but unlike many other Gram-negative bacteria, they lack the traditional peptidoglycan layer.

Within the species Treponema pallidum, there are several subspecies, including Treponema pallidum subsp. pallidum, which is the causative agent of syphilis. Other subspecies include Treponema pallidum subsp. pertenue, responsible for yaws, and Treponema pallidum subsp. endemicum, which causes bejel. Each of these subspecies is associated with distinct clinical manifestations and geographical distributions.

Structural Features:

Treponema pallidum is a relatively simple bacterium in terms of its structure. It has a flexible, helical shape that allows it to twist and move through various tissues within the host. It lacks the flagella found in many other bacteria and instead relies on an endoflagellum, an internal structure, for motility. The endoflagellum extends along the length of the bacterium and imparts the characteristic corkscrew-like movement.

The genome of Treponema pallidum is also relatively small, consisting of only about 1.14 million base pairs. This limited genetic material underscores the bacterium's reliance on its host for many essential nutrients.

Host Interactions:

Treponema pallidum is primarily a pathogen of humans. It is transmitted through various routes, with sexual contact being the most common for syphilis. Yaws and bejel, on the other hand, are typically transmitted through non-sexual contact in tropical and subtropical regions.

Upon infection, the bacterium undergoes several stages of development:

Primary Syphilis: This stage is marked by the appearance of a painless ulcer or sore, known as a chancre, at the site of infection.

Secondary Syphilis: If left untreated, the bacterium disseminates throughout the body, leading to a range of symptoms, including rashes, mucous membrane lesions, and fever.

Latent Syphilis: In this stage, the bacterium becomes dormant and can remain in the host for years without causing any noticeable symptoms.

Tertiary Syphilis: If the infection progresses to this stage, it can lead to severe complications, such as cardiovascular and neurological damage.

Yaws and bejel follow a somewhat different clinical course, typically involving skin and bone lesions, but they can also lead to severe complications if left untreated.

Associated Diseases:

Syphilis: Syphilis, caused by Treponema pallidum subsp. pallidum, is one of the most well-known sexually transmitted infections. It can affect various organ systems and presents a wide range of clinical manifestations, making it notorious for its ability to mimic other diseases. Syphilis is a major global health concern, and its early diagnosis and treatment are essential to prevent complications.

Yaws: Yaws, caused by Treponema pallidum subsp. pertenue, is a tropical disease primarily affecting the skin and bones. It presents as papillomatous skin lesions and can lead to disfigurement if left untreated. Yaws mainly affects children in tropical regions and is a significant public health concern in these areas.

Bejel: Bejel, associated with Treponema pallidum subsp. endemicum, is another non-venereal treponematosis characterized by skin and mucous membrane lesions. It typically occurs in arid and semi-arid regions and is most common among children.

Diagnostic Methods:

Accurate and timely diagnosis of Treponema infections is critical for effective treatment and disease management. Several diagnostic methods are available:

Serological Tests: These tests detect the presence of antibodies produced by the host in response to Treponema infections. The Venereal Disease Research Laboratory (VDRL) test and the Rapid Plasma Reagin (RPR) test are commonly used for the diagnosis of syphilis. These tests are sensitive and can detect both active and past infections.

Nucleic Acid Tests (NATs): Polymerase chain reaction (PCR) techniques are used to detect the genetic material of Treponema pallidum. The highly conserved TPN47 gene is often targeted in these tests because it is shared among different subspecies of Treponema. PCR assays offer high specificity and sensitivity, making them useful for confirming active infections.

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