Klebsiella pneumoniae & Pseudomonas aeruginosa antibody and antigen (recombinant protein)

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Diagnostic anti-Klebsiella pneumoniae & Pseudomonas aeruginosa antibodies pairs and antigen for animal health (animal Equine/Horse infectious disease K. pneumoniae often causes bacterial pneumonia; Pseudomonas aeruginosa causes urinary tract infections, respiratory system infections, dermatitis, soft tissue infections, bacteremia, bone and joint infections, gastrointestinal infections) testing in ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA and POCT

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Product information

Catalog No.	Description	US $ Price (per mg)
GMP-VT-P145-Ag01	Recombinant Klebsiella pneumoniae & Pseudomonas aeruginosa protein	$3090.00
GMP-VT-P145-Ab01	Anti-Klebsiella pneumoniae & Pseudomonas aeruginosa mouse monoclonal antibody (mAb)	$3090.00
GMP-VT-P145-Ab02	Anti-Klebsiella pneumoniae & Pseudomonas aeruginosa mouse monoclonal antibody (mAb)	$3090.00

Size： 1mg | 10mg | 100mg

Product Description

Cat No.	GMP-VT-P145-Ag01
Product Name	Recombinant Klebsiella pneumoniae & Pseudomonas aeruginosa protein
Pathogen	Klebsiella pneumoniae & Pseudomonas aeruginosa
Expression platform	E.coli
Isotypes	Recombinant Antigen
Bioactivity validation	Anti-Klebsiella pneumoniae & Pseudomonas aeruginosa antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in Klebsiella pneumoniae & Pseudomonas aeruginosa level test of animal Equine/Horse infectious disease with K. pneumoniae often causes bacterial pneumonia; Pseudomonas aeruginosa causes urinary tract infections, respiratory system infections, dermatitis, soft tissue infections, bacteremia, bone and joint infections, gastrointestinal infections.
Tag	His
Product description	Recombinant Klebsiella pneumoniae & Pseudomonas aeruginosa proteinwas expressed in E.coli - based prokaryotic cell expression system and is expressed with 6 HIS tag at the C-terminus.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in Sandwich ELISA, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No.	GMP-VT-P145-Ab01,GMP-VT-P145-Ab02
Pathogen	Klebsiella pneumoniae & Pseudomonas aeruginosa
Product Name	Anti-Klebsiella pneumoniae & Pseudomonas aeruginosa mouse monoclonal antibody (mAb)
Expression platform	CHO
Isotypes	Mouse IgG
Bioactivity validation	Recombinant Klebsiella pneumoniae & Pseudomonas aeruginosa antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Klebsiella pneumoniae & Pseudomonas aeruginosa antibodies in Klebsiella pneumoniae & Pseudomonas aeruginosa level test of animal Equine/Horse infectious disease with K. pneumoniae often causes bacterial pneumonia; Pseudomonas aeruginosa causes urinary tract infections, respiratory system infections, dermatitis, soft tissue infections, bacteremia, bone and joint infections, gastrointestinal infections.
Product description	Anti-Klebsiella pneumoniae & Pseudomonas aeruginosa mouse monoclonal antibody (mAb) is a mouse monoclonal antibody produced by CHO technology. The antibody is ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Klebsiella pneumoniae & Pseudomonas aeruginosa antibodies./td>
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in Sandwich ELISA, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Reference

Validation Data

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Pathogen

Klebsiella pneumoniae and Pseudomonas aeruginosa are two gram-negative prokaryotic bacteria that can cause serious infections in humans, animals, and plants. These pathogens have complex structures, each with unique virulence factors that allow them to evade and manipulate the host's defense mechanisms.

Klebsiella pneumoniae is a facultative anaerobe that belongs to the Enterobacteriaceae family and is commonly found in the human gut. This pathogen has an outer membrane consisting of lipopolysaccharides (LPSs), which play a crucial role in protecting the bacterium from the host's immune system. Additionally, Klebsiella pneumoniae has a polysaccharide capsule that surrounds the bacterium, known as the K antigen. This capsule is critical for the survival of the bacterium and helps it evade phagocytosis by macrophages and neutrophils.

The capsule of Klebsiella pneumoniae is composed of repeating units of sugar molecules called capsular polysaccharides, which are synthesized by genes located on the bacterial chromosome. The bacterium has over 80 different serotypes, each distinguished by its capsule composition. The serotype can influence the severity of the infection caused by Klebsiella pneumoniae, as well as the host's immune response to the bacterium.

In contrast, Pseudomonas aeruginosa is an aerobic bacterium that is commonly found in soil and water. This pathogen has a single polar flagellum that allows it to move efficiently through aqueous environments. Additionally, Pseudomonas aeruginosa has various virulence factors that allow it to adhere to host tissues and disrupt host cell function.

Pseudomonas aeruginosa's virulence factors include quorum-sensing genes, biofilm formation genes, exotoxins, and efflux pumps. Quorum sensing is a process by which bacteria communicate with each other and coordinate gene expression by secreting signaling molecules called autoinducers. Biofilm formation allows Pseudomonas aeruginosa to colonize surfaces and resist host defenses effectively. Exotoxins are toxic proteins secreted by the bacterium that can damage host cells, while efflux pumps are membrane transporters that enable the bacterium to pump out antimicrobial agents.

Both Klebsiella pneumoniae and Pseudomonas aeruginosa can infect humans and cause severe diseases. Klebsiella pneumoniae infections can cause pneumonia, urinary tract infections, wound infections, and bloodstream infections, particularly in hospitalized or immunocompromised patients. Pseudomonas aeruginosa primarily infects individuals with weakened immune systems, such as those with cystic fibrosis, and can cause infections in various parts of the body, including the lungs, skin, and urinary tract.

Diagnostic methods for these pathogens use culture-based techniques, serological assays, PCR-based methods, and nucleic acid sequencing. Culture-based techniques involve isolating and identifying the pathogen by growing it on selective media. Serological assays detect specific antibodies produced by the host in response to the pathogen. PCR-based methods detect DNA sequences from the pathogen using polymerase chain reaction, and nucleic acid sequencing can determine the pathogen's genetic makeup and susceptibility to antimicrobials.

In conclusion, both Klebsiella pneumoniae and Pseudomonas aeruginosa are gram-negative prokaryotic bacteria that can cause severe infections in humans and animals. These pathogens have complex structures with unique virulence factors that allow them to evade and manipulate the host's defense mechanisms. Diagnostic methods for these pathogens use culture-based techniques, serological assays, PCR-based methods, and nucleic acid sequencing, which target specific genes or proteins from the pathogens. Understanding the structures and unique virulence factors of these bacteria can help in developing therapeutic strategies to combat their infections effectively.

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