Brachyspira pilosicoli antibody and antigen (recombinant protein)

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Diagnostic anti-Brachyspira pilosicoli antibodies pairs and antigen for animal health (animal Swine/Porcine/Pig infectious disease porcine colonic spirochetosis) testing in ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA and POCT

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Product information

Catalog No.	Description	US $ Price (per mg)
GMP-VT-P129-Ag01	Recombinant Brachyspira pilosicoli protein	$3090.00
GMP-VT-P129-Ab01	Anti-Brachyspira pilosicoli mouse monoclonal antibody (mAb)	$3090.00
GMP-VT-P129-Ab02	Anti-Brachyspira pilosicoli mouse monoclonal antibody (mAb)	$3090.00

Size： 1mg | 10mg | 100mg

Product Description

Cat No.	GMP-VT-P129-Ag01
Product Name	Recombinant Brachyspira pilosicoli protein
Pathogen	Brachyspira pilosicoli
Expression platform	E.coli
Isotypes	Recombinant Antigen
Bioactivity validation	Anti-Brachyspira pilosicoli antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in Brachyspira pilosicoli level test of animal Swine/Porcine/Pig infectious disease with porcine colonic spirochetosis.
Tag	His
Product description	Recombinant Brachyspira pilosicoli proteinwas expressed in E.coli - based prokaryotic cell expression system and is expressed with 6 HIS tag at the C-terminus.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in Sandwich ELISA, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No.	GMP-VT-P129-Ab01,GMP-VT-P129-Ab02
Pathogen	Brachyspira pilosicoli
Product Name	Anti-Brachyspira pilosicoli mouse monoclonal antibody (mAb)
Expression platform	CHO
Isotypes	Mouse IgG
Bioactivity validation	Recombinant Brachyspira pilosicoli antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Brachyspira pilosicoli antibodies in Brachyspira pilosicoli level test of animal Swine/Porcine/Pig infectious disease with porcine colonic spirochetosis.
Product description	Anti-Brachyspira pilosicoli mouse monoclonal antibody (mAb) is a mouse monoclonal antibody produced by CHO technology. The antibody is ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Brachyspira pilosicoli antibodies./td>
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in Sandwich ELISA, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Reference

Validation Data

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Pathogen

Brachyspira pilosicoli is a bacterium that belongs to the phylum Spirochaetes and causes disease primarily in livestock, particularly pigs and poultry. B. pilosicoli is a prokaryotic bacterium that has an elongated spiral shape and measures around 2 to 6 micrometers in length and 0.1 to 0.3 micrometers in width. The bacteria are commonly found in the intestinal tract of these animals.

Bacterial pathogens can cause diseases in animals, including livestock, leading to reduced productivity, morbidity, and mortality. For instance, Brachyspira pilosicoli can cause swine dysentery, a highly contagious disease that leads to hemorrhagic colitis in pigs. In severe cases, the disease can be fatal, leading to significant economic losses for the pig industry.

In addition to pigs, avian species such as chickens, turkeys, and ostriches have also been found to be susceptible to infections by B. pilosicoli. Avian intestinal spirochetosis (AIS) is a result of this infection, and it is characterized by diarrhea, weight loss, and decreased feed conversion rate. AIS can impact poultry production by reducing growth rates, lower egg production, and increased mortality rates.

One of the essential properties of B. pilosicoli is its ability to attach to and invade the intestinal epithelial cells of its host. This process is aided by the expression of hemolysin, which helps with host tissue invasion. Hemolysin is a protein that is secreted by the bacterium and plays a critical role in allowing B. pilosicoli to cross the intestinal barrier and infect the host.

Moreover, motility is another important aspect of B. pilosicoli pathogenesis. FlaA, a surface protein, is responsible for the bacteria's motility, which helps them to colonize and move through the intestine. The flaA gene is conserved across many species of spirochetes and encodes flagellin, the structural unit of flagella.

Several diagnostic tests can be used to detect the presence of B. pilosicoli in animals. For instance, culture-based methods help in isolating the bacteria from stool or intestinal samples. PCR (polymerase chain reaction) is another technique that uses specific primers to amplify DNA sequences unique to B. pilosicoli. ELISA (enzyme-linked immunosorbent assay) is a useful serological method that detects specific antibodies produced against the pathogen.

Other nucleic acid-based tests, including qPCR (quantitative PCR) and LAMP (loop-mediated isothermal amplification), may also be used to detect B. pilosicoli. These tests are highly sensitive and specific and can accurately diagnose the pathogen in animals.

In conclusion, Brachyspira pilosicoli is a spiral-shaped bacterium belonging to the phylum Spirochaetes that causes disease primarily in pigs and poultry. The pathogen causes swine dysentery and avian intestinal spirochetosis, which can result in significant economic losses for livestock industries. Hemolysin and flaA genes and proteins play vital roles in B. pilosicoli's pathogenesis, aiding in the bacteria's attachment, invasion, and motility within the host. Various diagnostic techniques for detecting B. pilosicoli are available, such as culture-based methods, PCR, ELISA, qPCR, and LAMP.

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