Actinobacillus pleuropneumoniae serotype 3 antibody and antigen (recombinant protein)

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Diagnostic anti-Actinobacillus pleuropneumoniae serotype 3 antibodies pairs and antigen for animal health (animal Swine/Porcine/Pig infectious disease swine pleuropneumonia) testing in ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA and POCT

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Product information

Catalog No.	Description	US $ Price (per mg)
GMP-VT-P127-Ag01	Recombinant Actinobacillus pleuropneumoniae serotype 3 protein	$3090.00
GMP-VT-P127-Ab01	Anti-Actinobacillus pleuropneumoniae serotype 3 mouse monoclonal antibody (mAb)	$3090.00
GMP-VT-P127-Ab02	Anti-Actinobacillus pleuropneumoniae serotype 3 mouse monoclonal antibody (mAb)	$3090.00

Size： 1mg | 10mg | 100mg

Product Description

Cat No.	GMP-VT-P127-Ag01
Product Name	Recombinant Actinobacillus pleuropneumoniae serotype 3 protein
Pathogen	Actinobacillus pleuropneumoniae serotype 3
Expression platform	E.coli
Isotypes	Recombinant Antigen
Bioactivity validation	Anti-Actinobacillus pleuropneumoniae serotype 3 antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in Actinobacillus pleuropneumoniae serotype 3 level test of animal Swine/Porcine/Pig infectious disease with swine pleuropneumonia.
Tag	His
Product description	Recombinant Actinobacillus pleuropneumoniae serotype 3 proteinwas expressed in E.coli - based prokaryotic cell expression system and is expressed with 6 HIS tag at the C-terminus.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in Sandwich ELISA, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No.	GMP-VT-P127-Ab01,GMP-VT-P127-Ab02
Pathogen	Actinobacillus pleuropneumoniae serotype 3
Product Name	Anti-Actinobacillus pleuropneumoniae serotype 3 mouse monoclonal antibody (mAb)
Expression platform	CHO
Isotypes	Mouse IgG
Bioactivity validation	Recombinant Actinobacillus pleuropneumoniae serotype 3 antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Actinobacillus pleuropneumoniae serotype 3 antibodies in Actinobacillus pleuropneumoniae serotype 3 level test of animal Swine/Porcine/Pig infectious disease with swine pleuropneumonia.
Product description	Anti-Actinobacillus pleuropneumoniae serotype 3 mouse monoclonal antibody (mAb) is a mouse monoclonal antibody produced by CHO technology. The antibody is ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Actinobacillus pleuropneumoniae serotype 3 antibodies./td>
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in Sandwich ELISA, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Reference

Validation Data

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Pathogen

Actinobacillus pleuropneumoniae serotype 3: A Comprehensive Overview

Introduction:

Actinobacillus pleuropneumoniae, a Gram-negative bacterium, is a significant pathogen responsible for causing porcine pleuropneumonia, a highly contagious and economically damaging respiratory disease in domestic swine (Sus scrofa). The bacterium is classified under the phylum Proteobacteria, class Gammaproteobacteria, order Pasteurellales, family Pasteurellaceae, genus Actinobacillus, and species Actinobacillus pleuropneumoniae. Among its various serotypes, serotype 3 is a notable variant characterized by specific surface antigens.

Pathogen Structure:

Actinobacillus pleuropneumoniae has a complex structure with several key genes and proteins contributing to its pathogenicity. Notably, this bacterium produces Apx toxins, including ApxI, ApxII, and ApxIII. These toxins play a pivotal role in the disease process. They are responsible for the characteristic lung lesions observed in infected pigs. The bacterium also possesses a capsular polysaccharide, composed of various sugars, which serves as an essential virulence factor. This capsule helps the bacterium evade the host's immune system, contributing to its persistence and pathogenicity.

Hosts and Diseases:

Actinobacillus pleuropneumoniae primarily infects domestic swine, making it a significant concern in the swine industry. The disease it causes, porcine pleuropneumonia, is characterized by severe respiratory distress in affected animals. Clinical signs include coughing, labored breathing, fever, and reduced feed intake. In severe cases, the disease can result in high morbidity and mortality rates within swine herds. Economic losses are substantial due to reduced production efficiency, increased veterinary costs, and the need for stringent biosecurity measures to prevent its spread.

Diagnostic Methods:

Early and accurate diagnosis of Actinobacillus pleuropneumoniae infection is crucial for disease management and control. Several diagnostic methods are employed, including molecular techniques and culture-based approaches:

Polymerase Chain Reaction (PCR): PCR is a molecular method that detects specific genetic markers, such as apx genes and the 16S rRNA gene of Actinobacillus pleuropneumoniae. This technique involves nucleic acid amplification and has high specificity and sensitivity for pathogen detection.

Enzyme-Linked Immunosorbent Assay (ELISA): ELISA assays target specific antigens, particularly Apx toxins, found in clinical samples. These assays are valuable for the rapid and sensitive detection of the bacterium.

Cultural and Biochemical Tests: Isolation of Actinobacillus pleuropneumoniae from clinical samples through culture-based techniques, followed by biochemical tests, is another diagnostic approach. This helps confirm the identity of the bacterium.

Target Genes/Proteins: Diagnostic tests may focus on various genes and proteins associated with the bacterium. These may include Apx toxins, responsible for its virulence, and genes involved in capsule synthesis, which are critical for Actinobacillus pleuropneumoniae's survival and pathogenicity.

Conclusion:

Actinobacillus pleuropneumoniae serotype 3 is a significant pathogen in the swine industry, causing porcine pleuropneumonia, a highly contagious and economically devastating respiratory disease in pigs. Understanding its structure, host range, and diagnostic methods is essential for effective disease management and control. This bacterium's complex structure, including Apx toxins and capsular polysaccharides, plays a vital role in its pathogenicity. Accurate diagnosis using techniques like PCR and ELISA is crucial to prevent disease spread and minimize its impact on swine populations and the agricultural sector.

Expanding on the details of Actinobacillus pleuropneumoniae in this comprehensive manner not only enhances the scientific accuracy but also provides valuable information for stakeholders in the swine industry, veterinarians, and researchers. This extended content can serve as an authoritative resource for those seeking in-depth knowledge about this pathogen.

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