Pasteurella multocida antibody and antigen (recombinant protein)

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Diagnostic anti-Pasteurella multocida antibodies pairs and antigen for animal health (animal Swine/Porcine/Pig infectious disease respiratory disease in pig) testing in ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA and POCT

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Product information

Catalog No.	Description	US $ Price (per mg)
GMP-VT-P101-Ag01	Recombinant Pasteurella multocida protein	$3090.00
GMP-VT-P101-Ab01	Anti-Pasteurella multocida mouse monoclonal antibody (mAb)	$3090.00
GMP-VT-P101-Ab02	Anti-Pasteurella multocida mouse monoclonal antibody (mAb)	$3090.00

Size： 1mg | 10mg | 100mg

Product Description

Cat No.	GMP-VT-P101-Ag01
Product Name	Recombinant Pasteurella multocida protein
Pathogen	Pasteurella multocida
Expression platform	E.coli
Isotypes	Recombinant Antigen
Bioactivity validation	Anti-Pasteurella multocida antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in Pasteurella multocida level test of animal Swine/Porcine/Pig infectious disease with respiratory disease in pig.
Tag	His
Product description	Recombinant Pasteurella multocida proteinwas expressed in E.coli - based prokaryotic cell expression system and is expressed with 6 HIS tag at the C-terminus.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in Sandwich ELISA, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No.	GMP-VT-P101-Ab01,GMP-VT-P101-Ab02
Pathogen	Pasteurella multocida
Product Name	Anti-Pasteurella multocida mouse monoclonal antibody (mAb)
Expression platform	CHO
Isotypes	Mouse IgG
Bioactivity validation	Recombinant Pasteurella multocida antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Pasteurella multocida antibodies in Pasteurella multocida level test of animal Swine/Porcine/Pig infectious disease with respiratory disease in pig.
Product description	Anti-Pasteurella multocida mouse monoclonal antibody (mAb) is a mouse monoclonal antibody produced by CHO technology. The antibody is ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Pasteurella multocida antibodies./td>
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in Sandwich ELISA, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Reference

Validation Data

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Pathogen

Pasteurella multocida is a Gram-negative, facultative anaerobic bacterium that is commonly found in the respiratory tracts of domestic animals. P. multocida can infect a wide range of hosts, including domestic animals (such as dogs, cats, cattle, and pigs) and humans.

P. multocida is classified as a member of the Pasteurellaceae family and is the causative agent of various diseases in both animals and humans. In animals, it can cause fowl cholera, hemorrhagic septicemia, and pneumonia. Meanwhile, in humans, P. multocida can lead to localized infections like cellulitis, soft tissue infections, and respiratory infections, as well as systemic infections such as sepsis and meningitis.

The bacteria's virulence is attributed to its outer membrane proteins, such as OmpH, OmpA, and OmpW. These proteins are essential for the bacteria to adhere to and invade host cells. Furthermore, P. multocida produces several virulence factors that contribute to the disease process, including capsule polysaccharides, various toxins (such as dermonecrotic toxin), and lipopolysaccharides.

The primary gene in P. multocida is the toxA gene, which encodes the secreted toxin dermonecrotic toxin. The toxin's activity leads to tissue necrosis and contributes to the bacteria's virulence. In addition, P. multocida carries other genes that are essential for its survival in the host's environment. These genes include those that enable it to acquire iron from the host's tissues and those involved in capsule biosynthesis.

Clinical manifestations of P. multocida infections vary significantly depending on the host and the bacterial strain involved. In animals, the severity of the disease ranges from subclinical infections to fatal outcomes. In humans, the infections can be localized or systemic and range from mild to severe cases. For instance, P. multocida can cause cat and dog bites infections, which can range from mild cellulitis to severe sepsis.

Diagnostic methods for P. multocida include bacterial culture and biochemical tests, as well as PCR-based assays targeting specific genes such as the toxA gene. Other diagnostic methods include serological tests such as ELISA and Western blotting that detect antibodies against P. multocida antigens. In animals, the diagnosis of P. multocida infections is established using bacterial culture and isolation from infected tissues or blood samples. In humans, the diagnosis is usually made based on clinical presentation and a history of animal exposure.

The treatment of P. multocida infections involves the use of antibiotics such as penicillin, cephalosporins, and fluoroquinolones, depending on the disease severity and antibiotic susceptibility testing. In cases of severe infections such as sepsis and meningitis, hospitalization and supportive care may be necessary.

In conclusion, Pasteurella multocida is a gram-negative bacterium that causes various diseases in both animals and humans. The bacteria's virulence is attributed to its outer membrane proteins, toxins, and lipopolysaccharides. The primary gene in P. multocida is the toxA gene, encoding the dermonecrotic toxin that promotes tissue necrosis in infected hosts. Diagnostic methods for P. multocida infections include bacterial culture, biochemical tests, PCR-based assays, and serological tests. The treatment usually involves antibiotics, but severe infections may require hospitalization and supportive care.

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