Bovine Adenovirus-3 antibody and antigen (recombinant protein)

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Diagnostic anti-Bovine Adenovirus-3 antibodies pairs and antigen for animal health (animal Bovines/Cattle infectious disease respiratory and enteric infections) testing in ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA and POCT

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Product information

Catalog No.	Description	US $ Price (per mg)
GMP-VT-P069-Ag01	Recombinant Bovine Adenovirus-3 protein	$3090.00
GMP-VT-P069-Ab01	Anti-Bovine Adenovirus-3 mouse monoclonal antibody (mAb)	$3090.00
GMP-VT-P069-Ab02	Anti-Bovine Adenovirus-3 mouse monoclonal antibody (mAb)	$3090.00

Size： 1mg | 10mg | 100mg

Product Description

Cat No.	GMP-VT-P069-Ag01
Product Name	Recombinant Bovine Adenovirus-3 protein
Pathogen	Bovine Adenovirus-3
Expression platform	E.coli
Isotypes	Recombinant Antigen
Bioactivity validation	Anti-Bovine Adenovirus-3 antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in Bovine Adenovirus-3 level test of animal Bovines/Cattle infectious disease with respiratory and enteric infections.
Tag	His
Product description	Recombinant Bovine Adenovirus-3 proteinwas expressed in E.coli - based prokaryotic cell expression system and is expressed with 6 HIS tag at the C-terminus.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in Sandwich ELISA, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No.	GMP-VT-P069-Ab01,GMP-VT-P069-Ab02
Pathogen	Bovine Adenovirus-3
Product Name	Anti-Bovine Adenovirus-3 mouse monoclonal antibody (mAb)
Expression platform	CHO
Isotypes	Mouse IgG
Bioactivity validation	Recombinant Bovine Adenovirus-3 antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Bovine Adenovirus-3 antibodies in Bovine Adenovirus-3 level test of animal Bovines/Cattle infectious disease with respiratory and enteric infections.
Product description	Anti-Bovine Adenovirus-3 mouse monoclonal antibody (mAb) is a mouse monoclonal antibody produced by CHO technology. The antibody is ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Bovine Adenovirus-3 antibodies./td>
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in Sandwich ELISA, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Reference

Validation Data

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Pathogen

Bovine adenovirus-3 (BAdV-3) is a viral pathogen that primarily affects cattle and is known to cause respiratory disease. It belongs to the Adenoviridae family, which also includes human pathogens like adenovirus and canine pathogens like minute virus of canines (MVC). BAdV-3 is a non-enveloped virus with a capsid consisting of three major components: hexon, penton base, and fiber. The hexon protein accounts for over 90% of the capsid mass, while the penton base and fiber proteins are involved in receptor recognition and viral entry into host cells.

The genome of BAdV-3 is linear, double-stranded DNA with a size of approximately 34 kilobase pairs. It encodes more than 30 genes and can replicate within a variety of host cells, including bovine epithelia, placenta, and kidney. BAdV-3 has a broad tissue tropism, but it primarily targets the bovine respiratory epithelium.

The infection with BAdV-3 can cause a range of clinical symptoms, including fever, cough, nasal discharge, and dyspnea. These symptoms can vary from mild to severe and can last for several days up to a few weeks. The severity of the disease can be influenced by various factors, such as age, immune status, and environmental conditions.

Bovine adenovirus-associated respiratory disease (BAR) is the most common disease caused by BAdV-3 infection. Other manifestations of BAdV-3 infection in cattle include abortion, encephalitis, and enteritis. In addition to cattle, BAdV-3 has also been detected in other ruminants, such as buffalo and sheep. However, the pathogenicity of BAdV-3 in these species is not well understood.

Diagnosis of BAdV-3 infection can be challenging due to the broad range of clinical signs and co-infections with other pathogens. There are several diagnostic methods available for detecting BAdV-3 infection, including PCR, ELISA, and virus isolation. Among these, PCR and ELISA are widely used due to their sensitivity and specificity. PCR amplifies viral DNA sequences by specific primers and can detect low copy numbers of viral DNA in various tissues and secretions. ELISA detects anti-BAdV-3 antibodies in serum samples and can be used for serological surveillance and diagnosis.

Virus isolation involves propagating the virus within a host cell culture, allowing further characterization and study of the pathogen. However, this method is time-consuming and requires specialized facilities and expertise. Recently, next-generation sequencing (NGS) has become an important tool for identifying and characterizing viral genomes. NGS can provide whole-genome sequences of BAdV-3 and can be used for molecular epidemiology and surveillance of viral evolution and diversity.

In conclusion, BAdV-3 is an important viral pathogen that can cause respiratory disease and other manifestations in cattle and other ruminants. The diagnosis of BAdV-3 infection relies on multiple diagnostic methods, including PCR, ELISA, and virus isolation. Understanding the biology and pathogenesis of BAdV-3 is crucial for developing effective control and prevention strategies against this viral pathogen.

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