Dirofilaria immitis antibody and antigen (recombinant protein)

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Diagnostic anti-Dirofilaria immitis antibodies pairs and antigen for animal health (animal Dog/Canine infectious disease dirofilariasis) testing in ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA and POCT

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Product information

Catalog No.	Description	US $ Price (per mg)
GMP-VT-P024-Ag01	Recombinant Dirofilaria immitis protein	$3090.00
GMP-VT-P024-Ab01	Anti-Dirofilaria immitis mouse monoclonal antibody (mAb)	$3090.00
GMP-VT-P024-Ab02	Anti-Dirofilaria immitis mouse monoclonal antibody (mAb)	$3090.00

Size： 1mg | 10mg | 100mg

Product Description

Cat No.	GMP-VT-P024-Ag01
Product Name	Recombinant Dirofilaria immitis protein
Pathogen	Dirofilaria immitis
Expression platform	E.coli
Isotypes	Recombinant Antigen
Bioactivity validation	Anti-Dirofilaria immitis antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in Dirofilaria immitis level test of animal Dog/Canine infectious disease with dirofilariasis.
Tag	His
Product description	Recombinant Dirofilaria immitis proteinwas expressed in E.coli - based prokaryotic cell expression system and is expressed with 6 HIS tag at the C-terminus.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in Sandwich ELISA, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No.	GMP-VT-P024-Ab01,GMP-VT-P024-Ab02
Pathogen	Dirofilaria immitis
Product Name	Anti-Dirofilaria immitis mouse monoclonal antibody (mAb)
Expression platform	CHO
Isotypes	Mouse IgG
Bioactivity validation	Recombinant Dirofilaria immitis antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Dirofilaria immitis antibodies in Dirofilaria immitis level test of animal Dog/Canine infectious disease with dirofilariasis.
Product description	Anti-Dirofilaria immitis mouse monoclonal antibody (mAb) is a mouse monoclonal antibody produced by CHO technology. The antibody is ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-Dirofilaria immitis antibodies./td>
Purity	Purity: ≥95% (SDS-PAGE)
Application	Paired antibody immunoassay validation in Sandwich ELISA, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays.
Formulation	Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Reference

Validation Data

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Pathogen

Dirofilaria immitis is a parasitic nematode that infects primarily dogs, but also cats and wild canids such as foxes, wolves, and coyotes. It is transmitted by mosquitoes that carry infective third-stage larvae, which enter the host's bloodstream when the insect bites. Once inside the host, the larvae migrate to the right side of the heart and pulmonary arteries, where they develop into adult worms and mate, producing microfilariae that circulate in the bloodstream and can be picked up by mosquitoes.

Dirofilaria immitis has a unique morphology characterized by a long and slender body with a pointed tail. The adult worms can reach up to 30 cm in length and are often coiled in the pulmonary arteries, causing obstruction and damage to the heart and lungs. The pathogen is classified as eukaryotic and belongs to the phylum Nematoda.

The genome of Dirofilaria immitis contains several genes responsible for its survival within the host. The pathogen produces an enzyme called urease, which is involved in metabolism, and helps the pathogen adjust to the host's acidic environment. In addition, the pathogen produces several proteins such as heat shock proteins and antioxidant enzymes, which aid in survival and persistence within the host's body.

Heartworm disease caused by Dirofilaria immitis can have significant health consequences if left untreated. Infected animals may experience symptoms such as coughing, lethargy, and weight loss. Heart failure may occur in severe cases, leading to pulmonary hypertension and eventually death. The severity of the disease depends on several factors, such as the number of worms present, the duration of infection, and the animal's immune response.

Various diagnostic methods are available for detecting Dirofilaria immitis infection. The most common diagnostic tests include antibody tests, antigen tests, and nucleic acid tests. These methods target specific genes or proteins, such as the 16S ribosomal RNA gene, the Wolbachia surface protein, or the dihydrofolate reductase-thymidylate synthase (DHFR-TS) gene. Examples of diagnostic tests include the Knott test, the modified Knott test, and the polymerase chain reaction (PCR) test.

The Knott test is a basic microscopic test that involves concentrating microfilariae from a blood sample using a staining solution. The modified Knott test is a slight variation of the Knott test that uses a different staining solution and adds an additional centrifugation step to increase the sensitivity of the test. Both tests are relatively easy to perform and inexpensive but may not be as sensitive as other methods.

Antigen tests, on the other hand, detect the presence of adult female worm antigens in the bloodstream. These tests are more accurate than microfilarial tests but may have limitations in detecting very light infections or male-only infections.

Nucleic acid tests, such as the PCR test, detect the genetic material of the pathogen, making them the most sensitive and specific diagnostic tests available. These tests require specialized equipment and expertise, but they can detect the infection early and distinguish between active, past, and false-positive infections.

In conclusion, heartworm disease caused by Dirofilaria immitis is a significant health concern for dogs and other animals worldwide. The pathogen's unique morphology and survival mechanisms within the host's body make it challenging to diagnose and treat effectively. Nonetheless, with the availability of several diagnostic methods and preventive measures such as monthly heartworm preventives and mosquito control, heartworm disease can be prevented and managed effectively.

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