Trimethoprim(TMP) antibody/antigen (BSA/OVA/KLH conjugated hapten)

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Product information

Catalog No.	Description	US $ Price (per mg)
GMP-SMT-73-1	1. BSA-Trimethoprim(TMP) 2. Anti-Trimethoprim(TMP) mouse monoclonal antibody	$2709.00
GMP-SMT-73-2	1. OVA-Trimethoprim(TMP) 2. Anti-Trimethoprim(TMP) mouse monoclonal antibody	$2709.00
GMP-SMT-73-3	1. BSA-Trimethoprim(TMP) 2. Anti-Trimethoprim(TMP) human monoclonal antibody	$2709.00
GMP-SMT-73-4	1. OVA-Trimethoprim(TMP) 2. Anti-Trimethoprim(TMP) human monoclonal antibody	$2709.00
GMP-SMT-73-Ag-1	BSA-Trimethoprim(TMP)	$756.00
GMP-SMT-73-Ag-2	OVA-Trimethoprim(TMP)	$756.00
GMP-SMT-73-Ab-1	Anti-Trimethoprim(TMP) mouse monoclonal antibody	$1953.00
GMP-SMT-73-Ab-2	Anti-Trimethoprim(TMP) human monoclonal antibody	$1953.00

Size： 1mg | 10mg | 100mg

Product Description

BSA-Trimethoprim(TMP)

Cat No.	GMP-SMT-73-Ag-1
Bioactivity validation	Competitive immunoassay validation (Competitive ELISA) with hapten-carrier conjugates and anti-Hapten antibody;
Products description	Competitive immunoassay-validated hapten-carrier conjugates BSA-Trimethoprim(TMP) with anti-Hapten antibody. The hapten hapten-carrier conjugates BSA-Trimethoprim(TMP) had been validated with our anti-Hapten antibody Anti-Trimethoprim(TMP) mouse monoclonal antibody via competitive ELISA test.
Application	ELISA tests and other immunoassays; Lateral flow immunoassay (LFIA); LTIA Immunonephelometry Time-resolved Fluorescence Immunoassay (TRFIA)
Formulation & Reconstitution	Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

OVA-Trimethoprim(TMP)

Cat No.	GMP-SMT-73-Ag-2
Bioactivity validation	Competitive immunoassay validation (Competitive ELISA) with hapten-carrier conjugates and anti-Hapten antibody;
Products description	Competitive immunoassay-validated hapten-carrier conjugates OVA-Trimethoprim(TMP) with anti-Hapten antibody. The hapten hapten-carrier conjugates OVA-Trimethoprim(TMP) had been validated with our anti-Hapten antibody Anti-Trimethoprim(TMP) mouse monoclonal antibody via competitive ELISA test.
Application	ELISA tests and other immunoassays; Lateral flow immunoassay (LFIA); LTIA Immunonephelometry Time-resolved Fluorescence Immunoassay (TRFIA)
Formulation & Reconstitution	Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Anti-Trimethoprim(TMP) mouse monoclonal antibody

Cat No.	GMP-SMT-73-Ab-1
Host of Antibody	Mouse IgG
Bioactivity validation	Competitive immunoassay validation (Competitive ELISA) with hapten-carrier conjugates and anti-Hapten antibody; Lateral flow immunoassay (LFIA);
ELISA IC50 (ppb)	0.20
Products description	The anti-Hapten antibody against hapten Trimethoprim(TMP) had been validated with our hapten hapten-carrier conjugates BSA-Trimethoprim(TMP) via competitive ELISA test.
Application	ELISA tests and other immunoassays; Lateral flow immunoassay (LFIA); LTIA Immunonephelometry Time-resolved Fluorescence Immunoassay (TRFIA)
Formulation & Reconstitution	Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Anti-Trimethoprim(TMP) human monoclonal antibody

Cat No.	GMP-SMT-73-Ab-2
Host of Antibody	Human IgG1
Bioactivity validation	Competitive immunoassay validation (Competitive ELISA) with hapten-carrier conjugates and anti-Hapten antibody; Lateral flow immunoassay (LFIA);
ELISA IC50 (ppb)	0.20
Products description	The anti-Hapten antibody against hapten Trimethoprim(TMP) had been validated with our hapten hapten-carrier conjugates BSA-Trimethoprim(TMP) via competitive ELISA test.
Application	ELISA tests and other immunoassays; Lateral flow immunoassay (LFIA); LTIA Immunonephelometry Time-resolved Fluorescence Immunoassay (TRFIA)
Formulation & Reconstitution	Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Reference

Validation Data

Trimethoprim is a synthetic derivative of trimethoxybenzyl-pyrimidine with antibacterial and antiprotozoal properties. Sulfamethoxazole with trimethoprim is a fixed antibiotic combination that is widely used for mild-to-moderate bacterial infections and as prophylaxis against opportunistic infections. Recently, our R&D department demonstrated that our GMP-SMT-73-Ab-1 (Anti-Trimethoprim(TMP) mouse monoclonal antibody) has a large linear range and good sensitivity against the GMP-SMT-73-Ag-1 (BSA-Trimethoprim(TMP)). Below is the result of GeneMedi's Anti-Trimethoprim(TMP) mouse monoclonal antibody validation with BSA-Trimethoprim(TMP) in ELISA. We highly recommend the Ab&Ag to you.


Figure 1. GeneMedi's GMP-SMT-73-Ab-1 (Anti-Trimethoprim(TMP) mouse monoclonal antibody) is validated to detect the GMP-SMT-73-Ag-1 (BSA-Trimethoprim(TMP)) in ELISA. EC50 = 234.5 ng/ml.

Click to get more Data / Case study about the product.

Biomarker Information

1. Trimethoprim (TMP) Description:

1.Trimethoprim (TMP), scientifically identified as 2,4-diamino-5-(3,4,5-trimethoxybenzyl)pyrimidine, occupies a notable position within the realm of antimicrobial therapeutics. This synthetic antibacterial compound is classified under the dihydrofolate reductase inhibitors, a class renowned for its efficacy in combating bacterial infections. Here, we delve into a comprehensive understanding of TMP's chemical structure, mode of action, and its indispensable role in veterinary pharmacology.

Chemical Structure and Composition:

2.TMP presents a complex and intricate chemical structure. It is primarily characterized by a pyrimidine ring, a five-membered heterocyclic ring, harboring two amino groups at positions 2 and 4. Additionally, TMP features a benzyl ring with three methoxy (CH3O) groups at positions 3, 4, and 5. These structural components render TMP a potent antimicrobial agent with high specificity for its target, dihydrofolate reductase.

Mechanism of Action:

3.TMP's therapeutic prowess hinges on its ability to interfere with the bacterial folate synthesis pathway. Bacteria, much like their human counterparts, rely on the synthesis of folate (a B-vitamin) for the production of DNA, RNA, and proteins, critical for their growth and proliferation. Central to this process is the enzyme dihydrofolate reductase, which facilitates the conversion of dihydrofolic acid into tetrahydrofolic acid, a pivotal precursor in folate synthesis. TMP operates as a potent inhibitor of dihydrofolate reductase, disrupting the enzymatic conversion of dihydrofolic acid to tetrahydrofolic acid. As a consequence, the bacteria's ability to generate essential biomolecules is severely compromised, leading to bacteriostasis—the halting of bacterial growth.

Role in Veterinary Drug Residues and Additives (Serial Number 1):

4.Veterinary Drug Residues: TMP plays a pivotal role in the context of veterinary drug residues, particularly in the livestock and poultry industries. It is administered to animals as a therapeutic agent to combat bacterial infections. However, the presence of drug residues in animal-derived products such as meat, milk, and eggs poses a potential risk to human health. Monitoring TMP residues is, therefore, imperative to ensure the safety and quality of these consumable products. Regulatory bodies establish maximum residue limits (MRLs) for TMP, and exceeding these limits is a serious concern. Accurate measurement and control of TMP levels are critical for complying with these regulations and safeguarding public health.

5.Additives in Animal Feed: In addition to its role as a therapeutic agent, TMP may also be employed as an additive in animal feed formulations. The inclusion of TMP in animal diets serves a dual purpose—prevention and treatment. As a preventive measure, it helps control bacterial infections in livestock, reducing the occurrence of diseases that can significantly impact animal health and productivity. Furthermore, TMP in animal feed can be utilized to enhance the overall well-being of livestock by reducing the incidence of bacterial-related illnesses, which can be economically detrimental. By bolstering animal health, TMP contributes to the production of safe and high-quality animal-derived products.

6.Pharmacokinetics in Veterinary Medicine: Understanding the pharmacokinetics of TMP in veterinary medicine is critical to its effective and safe administration. TMP is absorbed through the gastrointestinal tract and reaches peak blood concentrations relatively quickly, making it a practical choice for oral administration. This rapid absorption is advantageous in combatting bacterial infections swiftly. It is then distributed throughout the body, allowing it to reach various tissues and bodily fluids, which is particularly important in the context of systemic infections. TMP is primarily excreted through the kidneys, which emphasizes the importance of proper dosage and considerations for animals with impaired renal function.

2. Reasons to Measure Trimethoprim (TMP) (Serial Number 2):

7.Ensuring Food Safety:

8.Rigorous and systematic quantification of TMP levels in animal-derived products serves as an indispensable facet of upholding the highest standards of food safety. The presence of TMP residues in these products must be closely monitored to ensure that they do not exceed established regulatory limits. This stringent surveillance not only safeguards consumer well-being but also underscores the commitment to delivering products that meet the most rigorous safety standards. Any breaches in these limits can have profound implications for public health, necessitating strict adherence to these measures.

9.Compliance with Stringent Regulations:

10.In the context of veterinary pharmacology and the use of TMP, compliance with stringent regulations is paramount. Regulatory authorities set forth guidelines and maximum residue limits (MRLs) for TMP in animal-derived products. These limits are established to protect consumers from potential health risks associated with excessive drug residues. Regular monitoring and quantification of TMP levels are, therefore, mandatory to ensure full compliance with these regulations. Such diligence not only prevents legal repercussions but also maintains the integrity and reputation of the biopharmaceutical company.

11.Optimizing Therapeutic Efficacy:

12.The accurate measurement of TMP concentrations in animals undergoing treatment is instrumental in fine-tuning therapeutic interventions. This precision allows veterinarians to tailor dosage regimens to individual animals, ensuring that the therapeutic effects of TMP are maximized. By avoiding underdosing, veterinarians can effectively combat bacterial infections while minimizing the risk of antibiotic resistance, which is a growing concern in both human and veterinary medicine.

13.Combatting Antibiotic Resistance:

14.The global emergence of antibiotic resistance is a pressing issue in healthcare and veterinary medicine. The overuse and misuse of antibiotics, including TMP, contribute to the development of resistant bacterial strains. Measuring TMP levels is a proactive strategy to combat this threat. By ensuring that TMP is used judiciously and in accordance with established guidelines, the veterinary industry plays a vital role in mitigating antibiotic resistance. The meticulous quantification of TMP levels supports responsible veterinary practices and aligns with the overarching objective of preserving the effectiveness of antibiotics for future generations.

15.Quality Control in Production:

16.For a biopharmaceutical enterprise specializing in the production of anti-TMP antibodies and small molecules competitive antigens, meticulous TMP measurement serves as a linchpin of quality assurance. It is paramount to ensure that these products meet rigorous standards and specifications, as they form an integral component of veterinary diagnostic and research applications. Consistency and accuracy in the production of these molecules are critical, as they directly impact the reliability and effectiveness of diagnostic tests, research studies, and immunological applications. By adhering to strict quality control measures in the production process, the biopharmaceutical company instills confidence among consumers, veterinarians, and researchers in the efficacy and precision of their offerings.

In conclusion, TMP, as a dihydrofolate reductase inhibitor, holds a prominent position in veterinary pharmacology. Its chemical structure and mechanism of action make it an effective antimicrobial agent. Its dual role in veterinary drug residues and as an animal feed additive underscores the importance of accurate measurement and monitoring. Ensuring food safety, regulatory compliance, therapeutic efficacy, and responsible antibiotic use are essential factors in the comprehensive management of TMP in veterinary medicine, and quality control in the production of related biopharmaceutical products is paramount for reliability and trust in the industry.

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