Cephalexin(CEX) antibody/antigen (BSA/OVA/KLH conjugated hapten)

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anti-Cephalexin(CEX) antibody and Carrier-coupled antigen/immunogen (hapten-carrier conjugates)

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Product information

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Catalog No.	Description	US $ Price (per mg)
GMP-SMT-67-1	1. BSA-Cephalexin(CEX) 2. Anti-Cephalexin(CEX) mouse monoclonal antibody	$2709.00
GMP-SMT-67-2	1. OVA-Cephalexin(CEX) 2. Anti-Cephalexin(CEX) mouse monoclonal antibody	$2709.00
GMP-SMT-67-3	1. BSA-Cephalexin(CEX) 2. Anti-Cephalexin(CEX) human monoclonal antibody	$2709.00
GMP-SMT-67-4	1. OVA-Cephalexin(CEX) 2. Anti-Cephalexin(CEX) human monoclonal antibody	$2709.00
GMP-SMT-67-5	1. BSA-Cephalexin(CEX) 2. Recombinant beta-lactams sensor protein	$2709.00
GMP-SMT-67-6	1. OVA-Cephalexin(CEX) 2. Recombinant beta-lactams sensor protein	$2709.00
GMP-SMT-67-Ag-1	BSA-Cephalexin(CEX)	$756.00
GMP-SMT-67-Ag-2	OVA-Cephalexin(CEX)	$756.00
GMP-SMT-67-Ab-1	Anti-Cephalexin(CEX) mouse monoclonal antibody	$1953.00
GMP-SMT-67-Ab-2	Anti-Cephalexin(CEX) human monoclonal antibody	$1953.00
GMP-SMT-sensor-1	Recombinant beta-lactams sensor protein	$1953.00

Size： 1mg | 10mg | 100mg

Product Description

BSA-Cephalexin(CEX)

Cat No.	GMP-SMT-67-Ag-1
Bioactivity validation	Competitive immunoassay validation (Competitive ELISA) with hapten-carrier conjugates and anti-Hapten antibody;
Products description	Competitive immunoassay-validated hapten-carrier conjugates BSA-Cephalexin(CEX) with anti-Hapten antibody. The hapten hapten-carrier conjugates BSA-Cephalexin(CEX) had been validated with our anti-Hapten antibody Anti-Cephalexin(CEX) mouse monoclonal antibody via competitive ELISA test.
Application	ELISA tests and other immunoassays; Lateral flow immunoassay (LFIA); LTIA Immunonephelometry Time-resolved Fluorescence Immunoassay (TRFIA)
Formulation & Reconstitution	Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

OVA-Cephalexin(CEX)

Cat No.	GMP-SMT-67-Ag-2
Bioactivity validation	Competitive immunoassay validation (Competitive ELISA) with hapten-carrier conjugates and anti-Hapten antibody;
Products description	Competitive immunoassay-validated hapten-carrier conjugates OVA-Cephalexin(CEX) with anti-Hapten antibody. The hapten hapten-carrier conjugates OVA-Cephalexin(CEX) had been validated with our anti-Hapten antibody Anti-Cephalexin(CEX) mouse monoclonal antibody via competitive ELISA test.
Application	ELISA tests and other immunoassays; Lateral flow immunoassay (LFIA); LTIA Immunonephelometry Time-resolved Fluorescence Immunoassay (TRFIA)
Formulation & Reconstitution	Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Anti-Cephalexin(CEX) mouse monoclonal antibody

Cat No.	GMP-SMT-67-Ab-1
Host of Antibody	Mouse IgG
Bioactivity validation	Competitive immunoassay validation (Competitive ELISA) with hapten-carrier conjugates and anti-Hapten antibody; Lateral flow immunoassay (LFIA);
ELISA IC50 (ppb)	0.4-0.5
Products description	The anti-Hapten antibody against hapten Cephalexin(CEX) had been validated with our hapten hapten-carrier conjugates BSA-Cephalexin(CEX) via competitive ELISA test.
Application	ELISA tests and other immunoassays; Lateral flow immunoassay (LFIA); LTIA Immunonephelometry Time-resolved Fluorescence Immunoassay (TRFIA)
Formulation & Reconstitution	Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Anti-Cephalexin(CEX) human monoclonal antibody

Cat No.	GMP-SMT-67-Ab-2
Host of Antibody	Human IgG1
Bioactivity validation	Competitive immunoassay validation (Competitive ELISA) with hapten-carrier conjugates and anti-Hapten antibody; Lateral flow immunoassay (LFIA);
ELISA IC50 (ppb)	0.4-0.5
Products description	The anti-Hapten antibody against hapten Cephalexin(CEX) had been validated with our hapten hapten-carrier conjugates BSA-Cephalexin(CEX) via competitive ELISA test.
Application	ELISA tests and other immunoassays; Lateral flow immunoassay (LFIA); LTIA Immunonephelometry Time-resolved Fluorescence Immunoassay (TRFIA)
Formulation & Reconstitution	Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Recombinant beta-lactams sensor protein

Cat No.	GMP-SMT-Sensor-1
Antigen & Antibody Name	Recombinant beta-lactams sensor protein
Expression platform	mammalian cell
Isotypes	Recombinant protein
Bioactivity validation	Competitive immunoassay validation (Competitive ELISA) with hapten-carrier conjugates and anti-Hapten antibody; Lateral flow immunoassay (LFIA);
Tag	mFc
Products description	Recombinant beta-lactams sensor protein was expressed in mammalian cell expression system with mFc tag at the C-terminus.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Antibody in Elisa, Competitive ELISA, lateral-flow tests, and other immunoassays
Formulation	Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Reference

Validation Data

Application development scientists of GDU (GeneMedi Diagnostics Unit) have generated and optimized the beta-lactams sensor protein with a larger linear range and better sensitivity against the beta-lactams. We tested 24 kinds of beta-lactams. Below is the competitive ELISA data of GeneMedi’s optimized beta-lactams sensor recombinant protein. For Ampicillin EC50 = 0.53 ng/ml (ppb); For Penicillin G, EC50 = 0.19 ng/ml (ppb). Our Recombinant beta-lactams sensor protein has obtained good performances, such as the stability and sensitivity.


Figure 1. GeneMedi's Recombinant beta-lactams sensor protein was validated to detect the beta-lactams (Ampicillin, Penicillin G, Cequinome, Cefapirin, Cephalexin, Ceftiofur) in ELISA.

Beta-lactams	Cross-Reactivity	Beta-lactams	Cross-Reactivity
Penicillin G	Y	Nafcillin	Y
Ampicillin	Y	Cephalothin	Y
Ceftiofur	Y	Cefapirin	Y
Cefquinome	Y	Cefoperazone	Y
Cephalosporin	Y	Cefotaxime	Y
Cephalexin	Y	Cefalotin	Y
Benzylpenicillin	Y	Cefuroxime	Y
Penicillin V	Y	Cefaclor	Y
Amoxicillin	Y	Ceftriaxone	Y
Oxacillin	Y	Cefamandole	Y
Cloxacillin	Y	Moxalactam	Y
Dicloxacillin	Y	Meropenem	Y
Kanamycin (Negative Control)	N	Tetracycline (Negative Control)	N

Table1. GeneMedi's Recombinant beta-lactams sensor protein can detect 24 kinds of beta-lactams with high sensitivity.

Click to get more Data / Case study about the product.

Biomarker Information

Cephalexin (CEX) Description and Its Role in Veterinary Drug Residues and Additives:

Cephalexin (CEX), a cephalosporin antibiotic, plays a pivotal role in the domain of veterinary therapeutics. This antimicrobial agent is renowned for its bactericidal properties and its ability to combat a broad spectrum of bacterial strains. Cephalexin's mechanism of action centers around inhibiting bacterial cell wall synthesis, a critical process that ensures the structural integrity and growth of bacteria. As such, it is highly effective in targeting and eradicating bacterial infections in animals.

Within the veterinary landscape, Cephalexin is extensively employed to address various bacterial infections in a myriad of species. It is often prescribed to manage infections such as dermatitis, urinary tract infections, respiratory infections, and more. Its popularity in the veterinary field is primarily attributable to its safety profile, reliability, and effectiveness in combating a wide range of bacterial pathogens.

However, as valuable as Cephalexin is for veterinary purposes, its utilization brings about challenges, particularly in the context of food safety and public health. One of the critical concerns associated with the administration of Cephalexin to animals is the potential for residues of this antibiotic to persist in edible animal products, including meat, milk, and eggs. This is a multifaceted issue that necessitates stringent monitoring and control to ensure the safety of these food products and, consequently, the health of consumers.

2. The Significance of Quantifying Cephalexin (CEX):

The quantification of Cephalexin (CEX) levels is of paramount importance across multiple domains, including veterinary medicine, food safety, regulatory compliance, antibiotic resistance mitigation, and public health. Here, we delve into each of these aspects in greater detail:

2.1. Animal Health Maintenance:

Accurate measurement and quantification of Cephalexin levels are fundamental in the veterinary sector to ensure the well-being of animals. The precision in dosing and administration of this antibiotic is crucial for achieving effective treatment outcomes. By adhering to proper dosing guidelines and monitoring the antibiotic's concentration in the animal's system, veterinarians can optimize the therapeutic effect of Cephalexin while minimizing the risk of antibiotic resistance development. This is particularly pertinent given the growing concern of antibiotic resistance in both animal and human healthcare.

2.2. Food Safety Assurance:

In the realm of food safety, regular monitoring and quantification of Cephalexin in food-producing animals are imperative. The antibiotic's residues can persist in consumable products, including meat, milk, and eggs, if not managed meticulously. Ensuring compliance with prescribed withdrawal periods is mandatory to prevent the presence of harmful residues in human diets. These residues can have severe health implications for consumers, ranging from allergic reactions to more significant threats like antibiotic resistance development, a global public health concern.

2.3. Regulatory Compliance:

Numerous regulatory standards have been established to safeguard the quality and safety of food products derived from animals. These standards often dictate permissible limits for antibiotic residues in animal-derived food items. Accurate quantification of Cephalexin levels is not only essential for meeting these regulatory requirements but also for demonstrating adherence to ethical and legal obligations. Failure to comply with these standards can have severe legal and reputational consequences for veterinary practitioners and the agricultural industry.

2.4. Antibiotic Resistance Mitigation:

The prudent management of antibiotics, such as Cephalexin, is integral to combating the global issue of antibiotic resistance. Misuse, overuse, or incorrect administration of antibiotics can facilitate the emergence of antibiotic-resistant bacterial strains, posing a substantial threat to both animal and human health. By quantifying Cephalexin levels and adhering to precise dosing regimens, the veterinary sector can play a significant role in mitigating antibiotic resistance, thus preserving the efficacy of antibiotics for future generations.

2.5. Public Health Safeguard:

Antibiotic residues in food products derived from animals present direct health risks to consumers. These risks encompass allergic reactions, which can be particularly severe in individuals with known antibiotic allergies. Moreover, the persistent exposure to low levels of antibiotics in the diet can contribute to the development of antibiotic resistance in humans. This alarming consequence underscores the necessity of quantifying Cephalexin levels in animal-derived food products, ultimately safeguarding public health.

In summary, the quantification of Cephalexin (CEX) is not only crucial for preserving animal health and ensuring food safety but also for complying with regulatory standards, mitigating antibiotic resistance, and safeguarding public health from the adverse consequences of antibiotic residues in animal-derived food products. It is an intricate process that harmonizes various aspects of veterinary medicine and public safety, underscoring the profound importance of precision and diligence in managing this antibiotic.

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