Ceftiofur(CEF) antibody/antigen (BSA/OVA/KLH conjugated hapten)

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anti-Ceftiofur(CEF) antibody and Carrier-coupled antigen/immunogen (hapten-carrier conjugates)

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Product information

Catalog No.	Description	US $ Price (per mg)
GMP-SMT-64-1	1. BSA-Ceftiofur(CEF) 2. Anti-Ceftiofur(CEF) mouse monoclonal antibody	$2709.00
GMP-SMT-64-2	1. OVA-Ceftiofur(CEF) 2. Anti-Ceftiofur(CEF) mouse monoclonal antibody	$2709.00
GMP-SMT-64-3	1. BSA-Ceftiofur(CEF) 2. Anti-Ceftiofur(CEF) human monoclonal antibody	$2709.00
GMP-SMT-64-4	1. OVA-Ceftiofur(CEF) 2. Anti-Ceftiofur(CEF) human monoclonal antibody	$2709.00
GMP-SMT-64-5	1. BSA-Ceftiofur(CEF) 2. Recombinant beta-lactams sensor protein	$2709.00
GMP-SMT-64-6	1. OVA-Ceftiofur(CEF) 2. Recombinant beta-lactams sensor protein	$2709.00
GMP-SMT-64-Ag-1	BSA-Ceftiofur(CEF)	$756.00
GMP-SMT-64-Ag-2	OVA-Ceftiofur(CEF)	$756.00
GMP-SMT-64-Ab-1	Anti-Ceftiofur(CEF) mouse monoclonal antibody	$1953.00
GMP-SMT-64-Ab-2	Anti-Ceftiofur(CEF) human monoclonal antibody	$1953.00
GMP-SMT-sensor-1	Recombinant beta-lactams sensor protein	$1953.00

Size： 1mg | 10mg | 100mg

Product Description

BSA-Ceftiofur(CEF)

Cat No.	GMP-SMT-64-Ag-1
Bioactivity validation	Competitive immunoassay validation (Competitive ELISA) with hapten-carrier conjugates and anti-Hapten antibody;
Products description	Competitive immunoassay-validated hapten-carrier conjugates BSA-Ceftiofur(CEF) with anti-Hapten antibody. The hapten hapten-carrier conjugates BSA-Ceftiofur(CEF) had been validated with our anti-Hapten antibody Anti-Ceftiofur(CEF) mouse monoclonal antibody via competitive ELISA test.
Application	ELISA tests and other immunoassays; Lateral flow immunoassay (LFIA); LTIA Immunonephelometry Time-resolved Fluorescence Immunoassay (TRFIA)
Formulation & Reconstitution	Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

OVA-Ceftiofur(CEF)

Cat No.	GMP-SMT-64-Ag-2
Bioactivity validation	Competitive immunoassay validation (Competitive ELISA) with hapten-carrier conjugates and anti-Hapten antibody;
Products description	Competitive immunoassay-validated hapten-carrier conjugates OVA-Ceftiofur(CEF) with anti-Hapten antibody. The hapten hapten-carrier conjugates OVA-Ceftiofur(CEF) had been validated with our anti-Hapten antibody Anti-Ceftiofur(CEF) mouse monoclonal antibody via competitive ELISA test.
Application	ELISA tests and other immunoassays; Lateral flow immunoassay (LFIA); LTIA Immunonephelometry Time-resolved Fluorescence Immunoassay (TRFIA)
Formulation & Reconstitution	Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Anti-Ceftiofur(CEF) mouse monoclonal antibody

Cat No.	GMP-SMT-64-Ab-1
Host of Antibody	Mouse IgG
Bioactivity validation	Competitive immunoassay validation (Competitive ELISA) with hapten-carrier conjugates and anti-Hapten antibody; Lateral flow immunoassay (LFIA);
ELISA IC50 (ppb)	4-5
Products description	The anti-Hapten antibody against hapten Ceftiofur(CEF) had been validated with our hapten hapten-carrier conjugates BSA-Ceftiofur(CEF) via competitive ELISA test.
Application	ELISA tests and other immunoassays; Lateral flow immunoassay (LFIA); LTIA Immunonephelometry Time-resolved Fluorescence Immunoassay (TRFIA)
Formulation & Reconstitution	Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Anti-Ceftiofur(CEF) human monoclonal antibody

Cat No.	GMP-SMT-64-Ab-2
Host of Antibody	Human IgG1
Bioactivity validation	Competitive immunoassay validation (Competitive ELISA) with hapten-carrier conjugates and anti-Hapten antibody; Lateral flow immunoassay (LFIA);
ELISA IC50 (ppb)	4-5
Products description	The anti-Hapten antibody against hapten Ceftiofur(CEF) had been validated with our hapten hapten-carrier conjugates BSA-Ceftiofur(CEF) via competitive ELISA test.
Application	ELISA tests and other immunoassays; Lateral flow immunoassay (LFIA); LTIA Immunonephelometry Time-resolved Fluorescence Immunoassay (TRFIA)
Formulation & Reconstitution	Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Recombinant beta-lactams sensor protein

Cat No.	GMP-SMT-Sensor-1
Antigen & Antibody Name	Recombinant beta-lactams sensor protein
Expression platform	mammalian cell
Isotypes	Recombinant protein
Bioactivity validation	Competitive immunoassay validation (Competitive ELISA) with hapten-carrier conjugates and anti-Hapten antibody; Lateral flow immunoassay (LFIA);
Tag	mFc
Products description	Recombinant beta-lactams sensor protein was expressed in mammalian cell expression system with mFc tag at the C-terminus.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Antibody in Elisa, Competitive ELISA, lateral-flow tests, and other immunoassays
Formulation	Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Reference

Validation Data

Application development scientists of GDU (GeneMedi Diagnostics Unit) have generated and optimized the beta-lactams sensor protein with a larger linear range and better sensitivity against the beta-lactams. We tested 24 kinds of beta-lactams. Below is the competitive ELISA data of GeneMedi’s optimized beta-lactams sensor recombinant protein. For Ampicillin EC50 = 0.53 ng/ml (ppb); For Penicillin G, EC50 = 0.19 ng/ml (ppb). Our Recombinant beta-lactams sensor protein has obtained good performances, such as the stability and sensitivity.


Figure 1. GeneMedi's Recombinant beta-lactams sensor protein was validated to detect the beta-lactams (Ampicillin, Penicillin G, Cequinome, Cefapirin, Cephalexin, Ceftiofur) in ELISA.

Beta-lactams	Cross-Reactivity	Beta-lactams	Cross-Reactivity
Penicillin G	Y	Nafcillin	Y
Ampicillin	Y	Cephalothin	Y
Ceftiofur	Y	Cefapirin	Y
Cefquinome	Y	Cefoperazone	Y
Cephalosporin	Y	Cefotaxime	Y
Cephalexin	Y	Cefalotin	Y
Benzylpenicillin	Y	Cefuroxime	Y
Penicillin V	Y	Cefaclor	Y
Amoxicillin	Y	Ceftriaxone	Y
Oxacillin	Y	Cefamandole	Y
Cloxacillin	Y	Moxalactam	Y
Dicloxacillin	Y	Meropenem	Y
Kanamycin (Negative Control)	N	Tetracycline (Negative Control)	N

Table1. GeneMedi's Recombinant beta-lactams sensor protein can detect 24 kinds of beta-lactams with high sensitivity.

Click to get more Data / Case study about the product.

Biomarker Information

1. Ceftiofur (CEF) in Veterinary Medicine

1.1 Definition and Mechanism of Action:

Ceftiofur (CEF), classified as a third-generation cephalosporin antibiotic, represents a crucial advancement in veterinary medicine. Its significance lies in its potent bactericidal properties, attributed to its ability to inhibit bacterial cell wall synthesis. By targeting the bacterial cell wall, Ceftiofur disrupts the structural integrity of a wide range of pathogens, including both Gram-positive and Gram-negative bacteria. This mechanism of action underscores its effectiveness against various bacterial strains, making it indispensable in the veterinary pharmacopeia.

1.2 Therapeutic Applications and Spectrum of Activity:

Ceftiofur finds extensive application in the treatment of bacterial infections afflicting livestock, such as cattle, swine, and poultry, as well as companion animals. Its versatility is exemplified in its efficacy against respiratory infections, skin disorders, and soft tissue injuries. Additionally, Ceftiofur plays a pivotal role in managing complex conditions like bovine respiratory disease in cattle, a prevalent and economically significant ailment. It is also employed in addressing metritis in dairy cattle, a condition crucial for maintaining herd health and productivity. Moreover, Ceftiofur is invaluable in combatting foot rot in sheep, a bacterial ailment affecting the hooves and causing lameness, thereby impacting animal welfare and productivity.

1.3 Residues and Regulatory Compliance:

The administration of Ceftiofur in animals necessitates strict adherence to withdrawal periods. These periods are mandated intervals between the last antibiotic dose and the time when the animal products, such as meat and milk, enter the human food chain. Monitoring Ceftiofur residues is imperative to ensure that these residues diminish to safe and permissible levels during the withdrawal period, aligning with stringent regulatory guidelines. This meticulous oversight is vital to prevent potential adverse effects on human health due to antibiotic residues in consumable animal products. Furthermore, regulatory compliance regarding Ceftiofur usage is paramount for the pharmaceutical industry, ensuring adherence to established standards and protocols governing antibiotic administration in animals.

2. The Significance of Measuring Ceftiofur (CEF)

2.1 Ensuring Food Safety:

Accurate measurement of Ceftiofur levels in animals is instrumental in upholding food safety standards. Monitoring and quantifying Ceftiofur residues in animal-derived products, including meat and milk, guarantee that these products are free from unsafe antibiotic residues. Consumption of such products could otherwise pose health risks to consumers, ranging from allergic reactions to the development of antibiotic-resistant bacteria. Rigorous testing protocols and precise measurement methods are imperative in this context, ensuring that animal products reaching the market are safe for human consumption.

2.2 Antibiotic Stewardship and Resistance Mitigation:

Measuring Ceftiofur levels also contributes significantly to antibiotic stewardship efforts. By understanding the extent of Ceftiofur usage and monitoring its residues, veterinarians and animal health professionals can make informed decisions regarding antibiotic prescriptions. This informed approach helps mitigate the development of antibiotic-resistant bacterial strains, a global concern that threatens the efficacy of antibiotics in both veterinary and human medicine.

2.3 Advancements in Detection Technology:

The field of detection technology has witnessed remarkable advancements, enabling the development of highly sensitive and specific methods for measuring Ceftiofur residues. Immunoassays, utilizing antibodies specifically designed to recognize Ceftiofur molecules, have revolutionized residue detection. Biopharmaceutical companies play a pivotal role in this domain, developing specialized antibodies and competitive antigens tailored for precise and sensitive detection methods. These innovations not only enhance the accuracy of Ceftiofur residue measurement but also contribute to the overall efficiency and reliability of food safety protocols.

2.4 Collaboration and Industry Leadership:

In the pursuit of ensuring food safety and mitigating antibiotic resistance, collaboration among regulatory bodies, research institutions, veterinary professionals, and biopharmaceutical companies is paramount. By fostering collaboration and industry leadership, stakeholders can collectively address challenges related to Ceftiofur usage and residues. Furthermore, industry leaders can drive research and development efforts to continually enhance detection methods, setting new benchmarks for precision and reliability in residue measurement.

In summary, Ceftiofur's role in veterinary medicine is multifaceted, encompassing therapeutic applications, regulatory compliance, and ensuring food safety. The meticulous measurement of Ceftiofur residues is indispensable, not only for regulatory adherence but also for antibiotic stewardship and mitigating the emergence of antibiotic-resistant bacteria. Through cutting-edge detection technologies and collaborative efforts, the veterinary and biopharmaceutical industries are at the forefront of safeguarding both animal and human health, thereby shaping a safer and more sustainable future.

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