tetracycline antibody/antigen (BSA/OVA/KLH conjugated hapten)

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Product information

Catalog No.	Description	US $ Price (per mg)
GMP-SMT-48-1	1. BSA-tetracycline 2. Anti-tetracycline mouse monoclonal antibody	$2709.00
GMP-SMT-48-2	1. OVA-tetracycline 2. Anti-tetracycline mouse monoclonal antibody	$2709.00
GMP-SMT-48-3	1. BSA-tetracycline 2. Anti-tetracycline human monoclonal antibody	$2709.00
GMP-SMT-48-4	1. OVA-tetracycline 2. Anti-tetracycline human monoclonal antibody	$2709.00
GMP-SMT-48-Ag-1	BSA-tetracycline	$756.00
GMP-SMT-48-Ag-2	OVA-tetracycline	$756.00
GMP-SMT-48-Ab-1	Anti-tetracycline mouse monoclonal antibody	$1953.00
GMP-SMT-48-Ab-2	Anti-tetracycline human monoclonal antibody	$1953.00

Size： 1mg | 10mg | 100mg

Product Description

BSA-tetracycline

Cat No.	GMP-SMT-48-Ag-1
Bioactivity validation	Competitive immunoassay validation (Competitive ELISA) with hapten-carrier conjugates and anti-Hapten antibody;
Products description	Competitive immunoassay-validated hapten-carrier conjugates BSA-tetracycline with anti-Hapten antibody. The hapten hapten-carrier conjugates BSA-tetracycline had been validated with our anti-Hapten antibody Anti-tetracycline mouse monoclonal antibody via competitive ELISA test.
Application	ELISA tests and other immunoassays; Lateral flow immunoassay (LFIA); LTIA Immunonephelometry Time-resolved Fluorescence Immunoassay (TRFIA)
Formulation & Reconstitution	Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

OVA-tetracycline

Cat No.	GMP-SMT-48-Ag-2
Bioactivity validation	Competitive immunoassay validation (Competitive ELISA) with hapten-carrier conjugates and anti-Hapten antibody;
Products description	Competitive immunoassay-validated hapten-carrier conjugates OVA-tetracycline with anti-Hapten antibody. The hapten hapten-carrier conjugates OVA-tetracycline had been validated with our anti-Hapten antibody Anti-tetracycline mouse monoclonal antibody via competitive ELISA test.
Application	ELISA tests and other immunoassays; Lateral flow immunoassay (LFIA); LTIA Immunonephelometry Time-resolved Fluorescence Immunoassay (TRFIA)
Formulation & Reconstitution	Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Anti-tetracycline mouse monoclonal antibody

Cat No.	GMP-SMT-48-Ab-1
Host of Antibody	Mouse IgG
Bioactivity validation	Competitive immunoassay validation (Competitive ELISA) with hapten-carrier conjugates and anti-Hapten antibody; Lateral flow immunoassay (LFIA);
ELISA IC50 (ppb)	0.3-0.5
Products description	The anti-Hapten antibody against hapten tetracycline had been validated with our hapten hapten-carrier conjugates BSA-tetracycline via competitive ELISA test.
Application	ELISA tests and other immunoassays; Lateral flow immunoassay (LFIA); LTIA Immunonephelometry Time-resolved Fluorescence Immunoassay (TRFIA)
Formulation & Reconstitution	Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Anti-tetracycline human monoclonal antibody

Cat No.	GMP-SMT-48-Ab-2
Host of Antibody	Human IgG1
Bioactivity validation	Competitive immunoassay validation (Competitive ELISA) with hapten-carrier conjugates and anti-Hapten antibody; Lateral flow immunoassay (LFIA);
ELISA IC50 (ppb)	0.3-0.5
Products description	The anti-Hapten antibody against hapten tetracycline had been validated with our hapten hapten-carrier conjugates BSA-tetracycline via competitive ELISA test.
Application	ELISA tests and other immunoassays; Lateral flow immunoassay (LFIA); LTIA Immunonephelometry Time-resolved Fluorescence Immunoassay (TRFIA)
Formulation & Reconstitution	Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Reference

Validation Data

Figure: GeneMedi's GMP-SMT-48-Ab-1 is validated to detect the GMP-SMT-48-Ag-1 in ELISA. IC50=0.390 ppb.

Click to get more Data / Case study about the product.

Biomarker Information

1. Tetracycline and Its Role in Veterinary Drug Residues and Additives

1.1 Introduction to Tetracycline

Tetracycline, a member of the tetracycline class of antibiotics, plays a pivotal role in veterinary medicine. It is a broad-spectrum antibiotic known for its effectiveness against a wide range of bacterial pathogens. This versatile antibiotic has found extensive application in the treatment and management of bacterial infections in livestock, poultry, and aquaculture.

1.2 Mechanism of Action

Tetracycline exerts its antibacterial effects by inhibiting bacterial protein synthesis. It does so by binding to the bacterial ribosome, preventing the attachment of aminoacyl-tRNA to the mRNA-ribosome complex. This disruption in protein synthesis leads to bacterial growth inhibition and eventual cell death. Its broad-spectrum nature makes it a valuable tool in addressing a variety of bacterial infections affecting animals.

1.3 The Significance of Tetracycline in Veterinary Medicine

Tetracycline is a cornerstone in the arsenal of antibiotics used in veterinary medicine. Its effectiveness against a multitude of bacterial pathogens makes it an essential tool for ensuring animal health. It is widely employed in the treatment of respiratory infections, enteric diseases, and a host of other bacterial maladies that can affect livestock, poultry, and fish in aquaculture.

1.4 Veterinary Drug Residues and Tetracycline

1.4.1 Residual Traces in Animal Products

When animals are treated with tetracycline-based medications, residual traces of the antibiotic can persist in various tissues, including muscle, liver, and fat. These residues can also find their way into animal products such as meat, milk, and eggs. It's essential to understand the dynamics of tetracycline residues to ensure food safety.

1.4.2 Maximum Residue Limits (MRLs)

To protect consumers and ensure food safety, regulatory agencies establish Maximum Residue Limits (MRLs) for tetracycline and other veterinary drugs. These MRLs dictate the permissible concentration of tetracycline residues in animal-derived products. Adherence to these limits is crucial to prevent adverse health effects and to maintain the integrity of the food supply chain.

2. The Significance of Tetracycline Measurement

2.1 Ensuring Food Safety

The precise measurement of tetracycline levels in animal-derived products is essential to guarantee the safety of our food sources. Excessive tetracycline residues in consumable products can pose health hazards to consumers. These hazards can range from allergic reactions to the development of antibiotic-resistant strains of bacteria, which can have serious public health implications.

2.2 Regulatory Compliance

Adhering to rigorous regulatory frameworks governing veterinary drug use is imperative for animal producers and the pharmaceutical industry. Regular measurement of tetracycline residues is essential to ensure compliance with these regulations. Failure to comply with MRLs can result in legal repercussions, recalls of products, and damage to a company's reputation. Additionally, compliance is vital for safeguarding public health by preventing the proliferation of antibiotic-resistant bacteria.

2.3 Mitigating Antibiotic Resistance

One of the most pressing global health concerns is the emergence of antibiotic-resistant bacteria. Overuse and misuse of antibiotics, including tetracycline, contribute to this issue. Precise measurement of tetracycline levels empowers veterinarians and farmers to use these drugs judiciously, thereby mitigating the menace of antibiotic resistance. Monitoring and measuring tetracycline residues help in optimizing antibiotic usage and minimizing the development of resistant bacterial strains.

2.4 Quality Assurance

Tetracycline measurement extends beyond food safety and regulatory compliance; it is also integral to quality assurance within the pharmaceutical and agricultural sectors. Accurate quantification of tetracycline levels is instrumental in upholding the quality and efficacy of products, whether they are veterinary medicines or animal feed additives. This ensures that the products meet the intended specifications, which is vital for animal health and productivity.

3. Tetracycline Measurement Techniques

3.1 High-Performance Liquid Chromatography (HPLC)

One of the most commonly used methods for tetracycline measurement is High-Performance Liquid Chromatography (HPLC). This technique involves the separation of tetracycline compounds in a complex mixture and their subsequent quantification. HPLC offers high sensitivity and specificity, making it a preferred choice in many laboratories and quality control settings.

3.2 Enzyme-Linked Immunosorbent Assay (ELISA)

Enzyme-Linked Immunosorbent Assay (ELISA) is another valuable method for tetracycline measurement. ELISA relies on the specificity of antibodies to detect and quantify tetracycline residues in samples. It is particularly useful when high-throughput screening of samples is required. ELISA is also relatively quick and cost-effective.

3.3 Liquid Chromatography-Mass Spectrometry (LC-MS)

Liquid Chromatography-Mass Spectrometry (LC-MS) is a highly advanced and sensitive technique for tetracycline measurement. It combines the separation capabilities of liquid chromatography with the mass spectrometry's high-resolution detection. LC-MS is effective in detecting tetracycline residues even at trace levels and is a valuable tool in research and regulatory monitoring.

4. Conclusion

In conclusion, tetracycline, a broad-spectrum antibiotic, is indispensable in veterinary medicine for treating bacterial infections in animals. Its residual presence in animal-derived products necessitates precise measurement to ensure food safety, regulatory compliance, and the mitigation of antibiotic resistance. Various techniques, including HPLC, ELISA, and LC-MS, offer reliable methods for quantifying tetracycline levels. Accurate measurement is a critical component of maintaining animal health, safeguarding the food supply chain, and preserving public health in an era of growing antibiotic resistance concerns.

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