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25-hydroxy (OH) Vitamin (25-OH-VD) antibody and antigen (hapten, BSA/OVA conjugated)
Diagnostic anti-25-hydroxy (OH) Vitamin (25-OH-VD) antibodies (anti-25-hydroxy (OH) Vitamin, anti-25-OH-VD) and diagnostic hapten-carrier conjugates BSA-25-hydroxy (OH) Vitamin (25-OH-VD), OVA-25-hydroxy (OH) Vitamin (25-OH-VD), KLH-25-hydroxy (OH) Vitamin (25-OH-VD) antigens for Metabolic diseases 25-OH-VD detection in ELISA test, competitive ELISA, Lateral flow immunoassay (LFIA), CLIA, TINIA and POCT.
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Product information
| Catalog No. | Description | US $ Price (per mg) |
|---|---|---|
| GMP-SMT-25-OH-VD-Ag01 | BSA-25-hydroxy (OH) Vitamin (25-OH-VD) | 756 |
| GMP-SMT-25-OH-VD-Ag02 | OVA-25-hydroxy (OH) Vitamin (25-OH-VD) | 756 |
| GMP-SMT-25-OH-VD-Ab01 | Anti-human 25-hydroxy (OH) Vitamin (25-OH-VD) mouse monoclonal antibody (mAb) | 1953 |
| GMP-SMT-25-OH-VD-Ab02 | Anti-human 25-hydroxy (OH) Vitamin (25-OH-VD) human monoclonal antibody (mAb) | 1953 |
Size： 1mg | 10mg | 100mg
Product Description
| Cat No. of Products | GMP-SMT-25-OH-VD-Ag01 |
| Product Name | BSA-25-hydroxy (OH) Vitamin (25-OH-VD) |
| Target/Biomarker | 25-hydroxy (OH) Vitamin (25-OH-VD) |
| Expression platform | Synthetic |
| Isotypes | NA |
| Bioactivity validation | 25-hydroxy (OH) Vitamin (25-OH-VD) antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in 25-OH-VD level test of Metabolic diseases (osteoporosis and rickets) and related syndrome evaluation. |
| Tag | NA |
| Products description | Competitive immunoassay-validated hapten-carrier conjugates BSA-25-hydroxy (OH) Vitamin (25-OH-VD) / OVA-25-hydroxy (OH) Vitamin (25-OH-VD) is Synthetic. |
| Purity | Purity: ≥95% (SDS-PAGE) |
| Application | Competitive immunoassay validation (Competitive ELISA), ELISA, Lateral flow immunoassay (LFIA),colloidal gold immunochromatographic assay, Chemiluminescent immunoassay (CLIA),turbidimetric inhibition immuno assay (TINIA) and Immunonephelometry. |
| Formulation & Reconstitution | Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O. |
| Storage | Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles. |
| Cat No. of Products | GMP-SMT-25-OH-VD-Ag02 |
| Product Name | OVA-25-hydroxy (OH) Vitamin (25-OH-VD) |
| Target/Biomarker | 25-hydroxy (OH) Vitamin (25-OH-VD) |
| Expression platform | Synthetic |
| Isotypes | NA |
| Bioactivity validation | 25-hydroxy (OH) Vitamin (25-OH-VD) antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in 25-OH-VD level test of Metabolic diseases (osteoporosis and rickets) and related syndrome evaluation. |
| Tag | NA |
| Products description | Competitive immunoassay-validated hapten-carrier conjugates BSA-25-hydroxy (OH) Vitamin (25-OH-VD) / OVA-25-hydroxy (OH) Vitamin (25-OH-VD) is Synthetic. |
| Purity | Purity: ≥95% (SDS-PAGE) |
| Application | Competitive immunoassay validation (Competitive ELISA), ELISA, Lateral flow immunoassay (LFIA),colloidal gold immunochromatographic assay, Chemiluminescent immunoassay (CLIA),turbidimetric inhibition immuno assay (TINIA) and Immunonephelometry. |
| Formulation & Reconstitution | Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O. |
| Storage | Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles. |
| Cat No. of Products | GMP-SMT-25-OH-VD-Ab01 |
| Product Name | Anti-human 25-hydroxy (OH) Vitamin (25-OH-VD) mouse monoclonal antibody (mAb) |
| Target/Biomarker | 25-hydroxy (OH) Vitamin (25-OH-VD) |
| Expression platform | Hybridoma |
| Isotypes | Mouse IgG |
| Bioactivity validation | 25-hydroxy (OH) Vitamin (25-OH-VD) antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in 25-OH-VD level test of Metabolic diseases (osteoporosis and rickets) and related syndrome evaluation. |
| Tag | mFc |
| Products description | Competitive immunoassay-validated hapten-carrier conjugates BSA-25-hydroxy (OH) Vitamin (25-OH-VD) / OVA-25-hydroxy (OH) Vitamin (25-OH-VD) is Synthetic. |
| Purity | Purity: ≥95% (SDS-PAGE) |
| Application | Competitive immunoassay validation (Competitive ELISA),Lateral flow immunoassay (LFIA),colloidal gold immunochromatographic assay, Chemiluminescent immunoassay (CLIA),turbidimetric inhibition immuno assay (TINIA) and Immunonephelometry. |
| Formulation & Reconstitution | Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O. |
| Storage | Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles. |
| Cat No. of Products | GMP-SMT-25-OH-VD-Ab02 |
| Product Name | Anti-human 25-hydroxy (OH) Vitamin (25-OH-VD) human monoclonal antibody (mAb) |
| Target/Biomarker | 25-hydroxy (OH) Vitamin (25-OH-VD) |
| Expression platform | Hybridoma |
| Isotypes | Mouse IgG |
| Bioactivity validation | 25-hydroxy (OH) Vitamin (25-OH-VD) antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in 25-OH-VD level test of Metabolic diseases (osteoporosis and rickets) and related syndrome evaluation. |
| Tag | mFc |
| Products description | Competitive immunoassay-validated hapten-carrier conjugates BSA-25-hydroxy (OH) Vitamin (25-OH-VD) / OVA-25-hydroxy (OH) Vitamin (25-OH-VD) is Synthetic. |
| Purity | Purity: ≥95% (SDS-PAGE) |
| Application | Competitive immunoassay validation (Competitive ELISA),Lateral flow immunoassay (LFIA),colloidal gold immunochromatographic assay, Chemiluminescent immunoassay (CLIA),turbidimetric inhibition immuno assay (TINIA) and Immunonephelometry. |
| Formulation & Reconstitution | Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O. |
| Storage | Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles. |
Reference
Validation Data
- Figure 1: Total 25-OH-VD identification ability
- Figure 2: Sample coincidence rate
- Figure 3: Standard curve
- Figure 4: Clinical comparative analysis
Figure 1. Total 25-OH-VD recognition ability; As can be seen from the figure above, 25-OH-VD2 and 25-OH-VD3 exhibit good cross-reactivity.
Genemedi's 25-OH-VD sandwich antibodies (GMP-SMT-25-OH-(VD3+VD2)-Ab01 and GMP-SMT-25-OH-(VD3+VD2)-Ab02) were validated on the direct chemiluminescence platform. The total amount of 25-OH-VD in the sample may be successfully detected thanks to the good cross-reactivity of 25-OH-VD2 and 25-OH-VD3.
Figure 2. Self-assessment clinical comparative analysis and Clinical comparative analysis of customer testing. The antibodies showed excellent agreement compared to mass spectrometry detection results, with an R2 as high as 0.9718 and a customer-validated R2 of 0.963. The sandwich approach greatly improves the accuracy of immunoassays.
The VD sandwich antibody(GMP-SMT-25-OH-(VD3+VD2)-Ab01 and GMP-SMT-25-OH-(VD3+VD2)-Ab02) made by Genemedi was validated using a direct chemiluminescence platform with the industry's gold standard LC-MS/MS as a control to increase the accuracy of the 25-OH-VD immunoassay. When compared to the mass spectrometry detection results, these antibodies exhibited excellent consistency, with an R2 of up to 0.9718 and a customer-verified R2 of 0.963. The sandwich approach greatly improved immunodetection accuracy.
Figure 3. Standard curve.
As can be
seen from the figure above, Genemedi's 25-OH-VD sandwich antibody has a
detection concentration range of 0.1 to 80ng/mL.
The detection concentration range for
Genemedi's 25-OH-VD sandwich antibodies (GMP-SMT-25-OH-(VD3+VD2)-Ab01 and
GMP-SMT-25-OH-(VD3+VD2)-Ab02) is 0.1 to 80ng/mL.
Figure 4. Clinical
comparative analysis(GMP-SMT-25-OH-(VD3+VD2)-Ab01 and GMP-SMT-25-OH-(VD3+VD2)-Ab02).
As can be seen from the figure above,
Detection employing 25-OH-VD sandwich antibodies validated by an enzymatic
chemiluminescence platform using an R2 of 0.9741, the data demonstrated strong
consistency using mass spectrometry (LC-MS/MS).
Click to get more Data / Case study about the product.
Target/Biomarker information
Vitamin D is a nutrient that is essential for healthy bones and teeth. There are two forms of vitamin D that are important for nutrition: vitamin D2 and vitamin D3. Vitamin D2 mainly comes from fortified foods like breakfast cereals, milk, and other dairy items. Vitamin D3 is made by your own body when you are exposed to sunlight. It is also found in some foods, including eggs and fatty fish, such as salmon, tuna, and mackerel. In your bloodstream, vitamin D2 and vitamin D3 are changed into a form of vitamin D called 25 hydroxyvitamin D, also known as 25 (OH)D. A vitamin D blood test measures the level of 25 (OH)D in your blood. Abnormal levels of vitamin D can indicate bone disorders, nutrition problems, organ damage, or other medical conditions. A vitamin D test is used to screen for or monitor bone disorders. It is also sometimes used to check vitamin D levels in people with chronic illnesses such as asthma, psoriasis, and certain autoimmune diseases.
About GDU
GDU helps global diagnostic partners in high quality of raw material discovery, development, and application. GDU believes in Protein&antibody Innovation for more reliable diagnostic solutions.
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