1,25-hydroxy (OH) Vitamin 3 (1,25-OH-VD3) antibody and antigen (hapten, BSA/OVA conjugated)

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Product information

Catalog No.	Description	US $ Price (per mg)
GMP-SMT-1-25-OH-VD3-Ag01	BSA-1,25-hydroxy (OH) Vitamin 3 (1,25-OH-VD3)	756
GMP-SMT-1-25-OH-VD3-Ag02	OVA-1,25-hydroxy (OH) Vitamin 3 (1,25-OH-VD3)	756
GMP-SMT-1-25-OH-VD3-Ab01	Anti-human 1,25-hydroxy (OH) Vitamin 3 (1,25-OH-VD3) mouse monoclonal antibody (mAb)	1953
GMP-SMT-1-25-OH-VD3-Ab02	Anti-human 1,25-hydroxy (OH) Vitamin 3 (1,25-OH-VD3) human monoclonal antibody (mAb)	1953
GMP-SMT-Sensor-5	Recombinant 1,25-OH-VD3 sensor protein	1953

Size： 1mg | 10mg | 100mg

Product Description

Cat No. of Products	GMP-SMT-1-25-OH-VD3-Ag01
Product Name	BSA-1,25-hydroxy (OH) Vitamin 3 (1,25-OH-VD3)
Target/Biomarker	1,25-hydroxy (OH) Vitamin 3 (1,25-OH-VD3)
Expression platform	Synthetic
Isotypes	NA
Bioactivity validation	1,25-hydroxy (OH) Vitamin 3 (1,25-OH-VD3) antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in 1,25-OH-VD3 level test of Metabolic diseases (osteoporosis and rickets) and related syndrome evaluation.
Tag	NA
Products description	Competitive immunoassay-validated hapten-carrier conjugates BSA-1,25-hydroxy (OH) Vitamin 3 (1,25-OH-VD3) / OVA-1,25-hydroxy (OH) Vitamin 3 (1,25-OH-VD3) is Synthetic.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Competitive immunoassay validation (Competitive ELISA), ELISA, Lateral flow immunoassay (LFIA),colloidal gold immunochromatographic assay, Chemiluminescent immunoassay (CLIA),turbidimetric inhibition immuno assay (TINIA) and Immunonephelometry.
Formulation & Reconstitution	Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No. of Products	GMP-SMT-1-25-OH-VD3-Ag02
Product Name	OVA-1,25-hydroxy (OH) Vitamin 3 (1,25-OH-VD3)
Target/Biomarker	1,25-hydroxy (OH) Vitamin 3 (1,25-OH-VD3)
Expression platform	Synthetic
Isotypes	NA
Bioactivity validation	1,25-hydroxy (OH) Vitamin 3 (1,25-OH-VD3) antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in 1,25-OH-VD3 level test of Metabolic diseases (osteoporosis and rickets) and related syndrome evaluation.
Tag	NA
Products description	Competitive immunoassay-validated hapten-carrier conjugates BSA-1,25-hydroxy (OH) Vitamin 3 (1,25-OH-VD3) / OVA-1,25-hydroxy (OH) Vitamin 3 (1,25-OH-VD3) is Synthetic.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Competitive immunoassay validation (Competitive ELISA), ELISA, Lateral flow immunoassay (LFIA),colloidal gold immunochromatographic assay, Chemiluminescent immunoassay (CLIA),turbidimetric inhibition immuno assay (TINIA) and Immunonephelometry.
Formulation & Reconstitution	Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No. of Products	GMP-SMT-1-25-OH-VD3-Ab01
Product Name	Anti-human 1,25-hydroxy (OH) Vitamin 3 (1,25-OH-VD3) mouse monoclonal antibody (mAb)
Target/Biomarker	1,25-hydroxy (OH) Vitamin 3 (1,25-OH-VD3)
Expression platform	Hybridoma
Isotypes	Mouse IgG
Bioactivity validation	1,25-hydroxy (OH) Vitamin 3 (1,25-OH-VD3) antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in 1,25-OH-VD3 level test of Metabolic diseases (osteoporosis and rickets) and related syndrome evaluation.
Tag	mFc
Products description	Competitive immunoassay-validated hapten-carrier conjugates BSA-1,25-hydroxy (OH) Vitamin 3 (1,25-OH-VD3) / OVA-1,25-hydroxy (OH) Vitamin 3 (1,25-OH-VD3) is Synthetic.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Competitive immunoassay validation (Competitive ELISA),Lateral flow immunoassay (LFIA),colloidal gold immunochromatographic assay, Chemiluminescent immunoassay (CLIA),turbidimetric inhibition immuno assay (TINIA) and Immunonephelometry.
Formulation & Reconstitution	Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No. of Products	GMP-SMT-1-25-OH-VD3-Ab02
Product Name	Anti-human 1,25-hydroxy (OH) Vitamin 3 (1,25-OH-VD3) human monoclonal antibody (mAb)
Target/Biomarker	1,25-hydroxy (OH) Vitamin 3 (1,25-OH-VD3)
Expression platform	Hybridoma
Isotypes	Mouse IgG
Bioactivity validation	1,25-hydroxy (OH) Vitamin 3 (1,25-OH-VD3) antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in 1,25-OH-VD3 level test of Metabolic diseases (osteoporosis and rickets) and related syndrome evaluation.
Tag	mFc
Products description	Competitive immunoassay-validated hapten-carrier conjugates BSA-1,25-hydroxy (OH) Vitamin 3 (1,25-OH-VD3) / OVA-1,25-hydroxy (OH) Vitamin 3 (1,25-OH-VD3) is Synthetic.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Competitive immunoassay validation (Competitive ELISA),Lateral flow immunoassay (LFIA),colloidal gold immunochromatographic assay, Chemiluminescent immunoassay (CLIA),turbidimetric inhibition immuno assay (TINIA) and Immunonephelometry.
Formulation & Reconstitution	Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No. of Products	GMP-SMT-Sensor-5
Product Name	Recombinant 1,25-OH-VD3 sensor protein
Target/Biomarker	1,25-hydroxy (OH) Vitamin 3 (1,25-OH-VD3)
Expression platform
Bioactivity validation	1,25-hydroxy (OH) Vitamin 3 (1,25-OH-VD3) antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in 1,25-OH-VD3 level test of Metabolic diseases (osteoporosis and rickets) and related syndrome evaluation.
Products description	Competitive immunoassay-validated hapten-carrier conjugates BSA-1,25-hydroxy (OH) Vitamin 3 (1,25-OH-VD3) / OVA-1,25-hydroxy (OH) Vitamin 3 (1,25-OH-VD3) is Synthetic.
Purity	Purity: ≥95% (SDS-PAGE)
Application	Competitive immunoassay validation (Competitive ELISA),Lateral flow immunoassay (LFIA),colloidal gold immunochromatographic assay, Chemiluminescent immunoassay (CLIA),turbidimetric inhibition immuno assay (TINIA) and Immunonephelometry.
Formulation & Reconstitution	Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage	Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Reference

Validation Data

The measurement of 1,25-dihydroxyvitamin D3 (1,25-OH-VD3), also known as calcitriol, is critical for evaluating the body's vitamin D status. As the hormonally active form of vitamin D, 1,25-OH-VD3 is essential for the regulation of calcium and phosphate metabolism, impacting bone health and immune function. Assessing its levels is particularly important in diagnosing and managing conditions such as osteoporosis, vitamin D deficiency, and parathyroid disorders.

GeneMedi's products offer a significant advantage in detecting 1,25-OH-VD3 through the use of a double-antibody sandwich ELISA method. This technique is superior to competitive assays for Higher Sensitivity and Specificity, Improved Accuracy, and Ease of Use.

1. Modified sandwich ELISA to detect 1,25-OH-VD3 (GMP-SMT-1,25-OH-VD3-Ag01)

Experimental procedure:

Coating – anti-1,25-OH-VD3 antibody (GMP-SMT-1,25-OH-VD3-Ab01) 1ug/ml, incubate overnight at 4℃; Blocking – 3% BSA, incubated 1h at 26℃ (RT);
1,25-OH-VD3 (GMP-SMT-1,25-OH-VD3-Ag01)/ Recombinant 1,25-OH-VD3 sensor protein (GMP-SMT-Sensor-5) preparation，mix 50ul of 1,25-OH-VD3 sensor protein with the 50 ul of 3-fold diluted 1,25-OH-VD3, The initial concentration of 1,25-OH-VD3 -100ng, incubate 1h at 26℃ (RT);
1,25-OH-VD3 (GMP-SMT-1,25-OH-VD3-Ag01)/ Recombinant 1,25-OH-VD3 sensor protein (GMP-SMT-Sensor-5) complex binding with anti-1,25-OH-VD3 antibody (GMP-SMT-1,25-OH-VD3-Ab01), Add 100ul of the 1,25-OH-VD3/ Recombinant 1,25-OH-VD3 sensor protein prepared above into the ELISA plate coated with anti-1,25-OH-VD3 antibody, incubate 1h at 26℃ (RT);
secondary antibody, 100 ul of Antiflag HRP (1:5000), incubate 1h at 26℃ (RT).

2.Modified sandwich ELISA – cross-reactivity checking

Experimental procedure:

Coating – anti-1,25-OH-VD3 antibody (GMP-SMT-1,25-OH-VD3-Ab01) 1ug/ml;
Blocking – 3% BSA;
In vitro Reactions
1, 25 OH VD3
2. 1,25-OH-VD3
3. 25 OH VD2
4. vitamin D3
5. vitamin D2
Fold of dilution – 3 fold -15 times;
Initial concentration -5ug;
Recombinant 1,25-OH-VD3 sensor protein (GMP-SMT-Sensor-5) – 0.5ug/ml;
Mix 100ul of diluted vitamin and 100ul of Recombinant 1,25-OH-VD3 sensor protein (GMP-SMT-Sensor-5) incubate 1h;
1,25-OH-VD3 (GMP-SMT-1,25-OH-VD3-Ag01)/ Recombinant 1,25-OH-VD3 sensor protein (GMP-SMT-Sensor-5) complex – 100ul of the invitro reacted samples;
Detection, 100 ul of Antiflag HRP (1:5000), Incubation temperature 26℃ (RT)/1h

Click to get more Data / Case study about the product.

Target/Biomarker information

Vitamin D is a nutrient that is essential for healthy bones and teeth. There are two forms of vitamin D that are important for nutrition: vitamin D2 and vitamin D3. Vitamin D2 mainly comes from fortified foods like breakfast cereals, milk, and other dairy items. Vitamin D3 is made by your own body when you are exposed to sunlight. It is also found in some foods, including eggs and fatty fish, such as salmon, tuna, and mackerel. In your bloodstream, vitamin D2 and vitamin D3 are changed into a form of vitamin D called 25 hydroxyvitamin D, also known as 25 (OH)D. A vitamin D blood test measures the level of 25 (OH)D in your blood. Abnormal levels of vitamin D can indicate bone disorders, nutrition problems, organ damage, or other medical conditions. A vitamin D test is used to screen for or monitor bone disorders. It is also sometimes used to check vitamin D levels in people with chronic illnesses such as asthma, psoriasis, and certain autoimmune diseases.

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