Feline cTnI antibody and antigen (recombinant protein)

Diagnostic anti-Feline cTnI antibodies pairs and antigen for animal health (animal Cat/Feline Myocardial injury, heart disease) testing in ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA and POCT

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Product information

Catalog No. Description US $ Price (per mg)
GMP-FEL-cTnI-Ag01 Recombinant Feline cTnI protein 3090
GMP-FEL-cTnI-Ab01 Anti-Feline cTnI mouse monoclonal antibody (mAb) 1953
GMP-FEL-cTnI-Ab02 Anti-Feline cTnI mouse monoclonal antibody (mAb) 1953
GMP-FEL-cTnI-Ab03 Anti-Feline cTnI human monoclonal antibody (mAb) 1953
GMP-FEL-cTnI-Ab04 Anti-Feline cTnI human monoclonal antibody (mAb) 1953

Size: 1mg | 10mg | 100mg



Product Description

Cat No. of Products GMP-FEL-cTnI-Ag01
Product Name Recombinant Feline cTnI protein
Target/Biomarker Feline Cardiac troponin I (cTnI)
Expression platform E.coli
Isotypes Recombinant Antigen
Bioactivity validation Anti-Feline Cardiac troponin I antibodies binding, Immunogen in Sandwich Elisa, lateral-flow tests, and other immunoassays as control material in cTnI level test of animal Cat/Feline with Myocardial injury, heart disease.
Tag His
Products description Recombinant Feline cTnI protein was expressed in E.coli - based prokaryotic cell expression system and is expressed with 6 HIS tag at the C-terminus.
Purity Purity: ≥95% (SDS-PAGE)
Application Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays
Formulation & Reconstitution Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No. of Products GMP-FEL-cTnI-Ab01, GMP-FEL-cTnI-Ab02
Product Name Anti-Feline cTnI mouse monoclonal antibody (mAb)
Target/Biomarker Feline Cardiac troponin I (cTnI)
Expression platform CHO
Isotypes Mouse IgG
Bioactivity validation Recombinant Feline Cardiac troponin I antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-cTnI antibodies in cTnI level test of animal Cat/Feline with Myocardial injury, heart disease.
Tag mFc
Products description Anti-Feline cTnI mouse monoclonal antibody (mAb) is a monoclonal antibody produced by CHO technology. The antibody is ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-cTnI antibodies.
Purity Purity: ≥95% (SDS-PAGE)
Application Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays
Formulation & Reconstitution Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Cat No. of Products GMP-FEL-cTnI-Ab03, GMP-FEL-cTnI-Ab04
Product Name Anti-Feline cTnI human monoclonal antibody (mAb)
Target/Biomarker Feline Cardiac troponin I (cTnI)
Expression platform CHO
Isotypes Human IgG1
Bioactivity validation Recombinant Feline Cardiac troponin I antigen binding, ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-cTnI antibodies in cTnI level test of animal Cat/Feline with Myocardial injury, heart disease.
Tag hFc
Products description Anti-Feline cTnI human monoclonal antibody (mAb) is a monoclonal antibody produced by CHO technology. The antibody is ELISA validated as capture antibody and detection antibody. Pair recommendation with other anti-cTnI antibodies.
Purity Purity: ≥95% (SDS-PAGE)
Application Paired antibody immunoassay validation in sandwich Elisa, ELISA, colloidal gold-based Lateral flow immunoassay (LFIA), CLIA, TINIA, POCT and other immunoassays
Formulation & Reconstitution Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4); For PSB2, reconstituted with 0.9% sodium chloride; For PBS, reconstituted with ddH2O.
Storage Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.

Reference




    Validation Data


    GeneMedi's feline cTnI antibodies exhibit superior performance in sandwich immunoassays.

    GeneMedi’s GMP-FEL-cTnI-Ab01, GMP-FEL-cTnI-Ab02, and GMP-FEL-cTnI-Ab05 demonstrate superior performance in sandwich ELISA assays. We tested four antibody pairs, and the GMP-FEL-cTnI-Ab01 and GMP-FEL-cTnI-Ab02 pair showed the lowest EC50. Based on these results, we recommend this antibody pair for optimal performance in cTnI detection.

    Figure 1. The performance of GeneMedi’s feline cTnI antibodies in sandwich ELISA assays.

    A-B. Results of four different antibody pairs in sandwich ELISA assays.
    C. Antibody pairs and their EC50 values.

    cTnI validation

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    Click to get more Data / Case study about the product.



    Target/Biomarker information

    Cardiac Troponin I (cTnI) is a protein of exceptional significance in the context of feline physiology. This cardiac-specific biomarker is predominantly found in the bloodstream of cats (felines) and serves as a crucial indicator of cardiac health. To truly appreciate the role of cTnI, it is essential to delve into its structural composition, functional significance, and its position within the realm of feline cardiology. Structurally, cTnI is one of the three subunits of the troponin complex, the other two being cardiac Troponin T (cTnT) and cardiac Troponin C (cTnC). The troponin complex resides within the sarcomeres of cardiac muscle fibers, where it meticulously regulates muscle contraction. This precision is vital for the rhythmic and efficient pumping of blood by the heart. What distinguishes cTnI is its exclusive cardiac specificity. While other troponin isoforms exist in skeletal muscle, cTnI is uniquely expressed in the heart. This specificity makes cTnI an exceptionally valuable biomarker for assessing cardiac muscle injury or damage. When cardiac cells are compromised due to factors such as myocardial infarction, cardiac diseases, or cardiac trauma, cTnI is released into the bloodstream. Therefore, elevated levels of cTnI in a cat's blood can serve as a critical diagnostic indicator of cardiac health. Detecting cTnI levels in feline serum is typically achieved through immunoassays, such as enzyme-linked immunosorbent assays (ELISA). These assays provide a quantitative measure of cTnI concentration, enabling veterinarians to assess the extent of cardiac injury or disease. The sensitivity of these assays is such that even minor myocardial damage can lead to detectable elevations in cTnI levels, allowing for early diagnosis and intervention.



    About GDU


    GDU

    GDU helps global diagnostic partners in high quality of raw material discovery, development, and application. GDU believes in Protein&antibody Innovation for more reliable diagnostic solutions.

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