Equine SAA antibody and antigen (recombinant protein)

GeneMedi’s anti-SAA monoclonal antibodies demonstrate superior performance in immunoassay.

GeneMedi’s monoclonal antibodies for SAA (Serum Amyloid A) offer high sensitivity for detecting SAA in cat, dog, and horse serum. Due to the high sequence homology among these species, the same antibody pairs can be used to develop versatile sandwich immunoassays. As a result, GeneMedi’s anti-SAA antibodies provide a valuable tool for accurately monitoring and diagnosing inflammatory conditions across multiple species.

Figure 1. GeneMedi’s monoclonal antibodies demonstrate high sensitivity and specificity for detecting SAA in both healthy and inflammatory cat plasma samples using a sandwich immunoassay.

A. Calibration curve for GMP-FEL-Serum-amyloid-A-Ag01 in mixed cat plasma in the sandwich immunoassay. The SAA concentration in mixed cat plasma was 145 μg/ml, and the sample was diluted 208 times prior to testing.
B-C. Dilution curves for recombinant cat SAA antigen and plasma samples using the prototype reagents in the sandwich immunoassay. (B) Healthy cat samples (H1, H2), with SAA concentrations of 0.67 μg/ml and 0.45 μg/ml, respectively. Plasma samples were diluted 45 times before testing. The initial concentration of GMP-FEL-Serum-amyloid-A-Ag01 was 65 ng/ml. (C) Postoperative cat samples (P1, P2), with SAA concentrations of 169 μg/ml and 220 μg/ml, respectively. Plasma samples were diluted 200 times before testing. The initial concentration of GMP-FEL-Serum-amyloid-A-Ag01 was 200 ng/ml.
D. SAA concentrations in healthy and postoperative inflammatory cats. Healthy cat samples were pre-diluted 100-3000 times, while inflammatory cat samples were pre-diluted 9000 times prior to testing.

Figure 2. GeneMedi’s anti-SAA monoclonal antibodies enable sensitive detection of SAA in dog, horse, and cat serum using a sandwich immunoassay.

A-B. Dilution curves of recombinant dog (A) and horse (B) SAA antigens in the sandwich immunoassay. GMP-CAN-Serum-amyloid-A-Ab01 was coated and blocked at 37°C for 1 hour, and incubated with GMP-CAN-Serum-amyloid-A-Ag01 and GMP-EQU-Serum-amyloid-A-Ag01 at 37°C for 1 hour.
C. The recommended antibody pairs.
D-E. Comparison of SAA detection results between diseased and healthy individuals in dogs (D) and horses (E) in the sandwich immunoassay. Healthy serum was pre-diluted 50 times, while diseased serum was pre-diluted 1500 times (horse) or 2500 times (dog), with the dilution buffer containing 1% casein and 0.05% Tween 20.
F-H. Testing results of SAA in cat (F), dog (G), and horse (H) serum samples in the sandwich immunoassay. GMP-FEL-Serum-amyloid-A-Ab10 was blocked for 1 hour (blocking solution containing 2.5% sodium caseinate). Dog serum was pre-diluted 2500 times, while cat and horse serum were pre-diluted 1500 times, using a Tris buffer containing 0.01% CHAPS.