GeneMedi Anti-FLAG Mouse Monoclonal Antibody (M2 epitope)

GeneMedi's Anti-FLAG Mouse Monoclonal Antibody (M2 epitope) is a high-quality, rigorously tested antibody specifically designed for accurate and efficient detection of FLAG-tagged proteins. Derived from mouse monoclonal sources, this antibody ensures high specificity and affinity towards FLAG epitope DYKDDDDK, a widely used tag in protein expression studies. GM’s anti-FLAG mab recognizes the same epitope as the M2 antibody.

Order information

Catalog No. Description Size US $ Price
GMLS-Tag001-Ab-1 Anti-FLAG Mouse Monoclonal Antibody (M2 epitope) 100ul 190
GMLS-Tag001-Ab-1 Anti-FLAG Mouse Monoclonal Antibody (M2 epitope) 1ml 1513
GMLS-Tag001-Ab-1 Anti-FLAG Mouse Monoclonal Antibody (M2 epitope) 10ml 15106
GMLS-Tag001-Ab-1 Anti-FLAG Mouse Monoclonal Antibody (M2 epitope) >10ml Inquiry
GMLS-Tag001-Ab-1-HRP HRP-conjugated Anti-FLAG Mouse Monoclonal Antibody (M2 epitope) 100ul 256
GMLS-Tag001-Ab-1-HRP HRP-conjugated Anti-FLAG Mouse Monoclonal Antibody (M2 epitope) 1ml 2048
GMLS-Tag001-Ab-1-HRP HRP-conjugated Anti-FLAG Mouse Monoclonal Antibody (M2 epitope) 10ml 16425
GMLS-Tag001-Ab-1-HRP HRP-conjugated Anti-FLAG Mouse Monoclonal Antibody (M2 epitope) >10ml Inquiry

For more than 100mg, click here for inquiry. >>


Description


Anti-FLAG Mouse Monoclonal Antibody (M2 epitope)

Cat No. GMLS-Tag001-Ab-1
Product Name Anti-FLAG Mouse Monoclonal Antibody (M2 epitope)
Target FLAG
Expression platform mammalian cell
Isotypes Mouse IgG
Bioactivity validation FLAG binding, Competitive immunoassay validation (Competitive ELISA) in FLAG level test.
Tag mFc
Products description Anti-FLAG mouse monoclonal antibody (M2 Epitope) is expressed from mammalian cell.
Species Mouse
Purity Purity: ≥95% （SDS-PAGE）
Application Western Blotting, Immunoprecipitation (IP), Immunofluorescence (IF), Flow Cytometry, and Immunohistochemistry (IHC) for accurate and efficient detection of FLAG-tagged proteins.
Formulation & Reconstitution Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4);
For PSB2, reconstituted with 0.9% sodium chloride;
For PBS, reconstituted with ddH2O.
Storage Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.


HRP-conjugated Anti-FLAG Mouse Monoclonal Antibody (M2 epitope)

Cat No. GMLS-Tag001-Ab-1-HRP
Product Name HRP-conjugated Anti-FLAG Mouse Monoclonal Antibody (M2 epitope)
Target FLAG
Expression platform mammalian cell
Isotypes Mouse IgG
Bioactivity validation FLAG binding, Competitive immunoassay validation (Competitive ELISA) in FLAG level test.
Tag mFc
Products description Anti-FLAG mouse monoclonal antibody (M2 Epitope) is expressed in mammalian cells and conjugated with HRP.
Species Mouse
Purity Purity: ≥95% （SDS-PAGE）
Application Western Blotting, Immunoprecipitation (IP), Flow Cytometry, and ELISA for accurate and efficient detection of FLAG-tagged proteins.
Formulation & Reconstitution PBS, pH7.4 with protectant.
Storage Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.


Key Applications

  1. Western Blotting: Ideal for detecting FLAG-tagged proteins in SDS-PAGE-separated samples.

  2. Immunoprecipitation (IP): Excellently suited for isolating FLAG-tagged proteins from complex mixtures, facilitating downstream analysis.

  3. Immunofluorescence (IF): Useful in localizing FLAG-tagged proteins within cells, aiding in cellular biology studies.

  4. Flow Cytometry: Enables the identification and analysis of FLAG-tagged proteins in suspension cells.

  5. Immunohistochemistry (IHC): Applicable for detecting and visualizing FLAG-tagged proteins in tissue sections.



Why Choose GeneMedi's Product?

  1. Consistency and Reliability: Each batch undergoes stringent quality control tests, ensuring consistent performance.

  2. High Sensitivity: Optimized to detect even low levels of FLAG-tagged proteins, ensuring no target goes unnoticed.

  3. Reduced Background Noise: Minimizes non-specific bindings, leading to clear and interpretable results.

  4. Versatility: Compatible with a variety of sample types and research applications.

  5. Support and Resources: GeneMedi provides comprehensive technical support and detailed product documentation to assist researchers in their experiments.


Validation Data


GeneMedi's Anti-FLAG antibody demonstrates superior performance in immunoblot and FACS analysis


GeneMedi’s GMLS-Tag001-Ab-1 anti-FLAG Mouse Monoclonal Antibody (M2 epitope) demonstrates superior performance in western blot and FACS assays compared to other commercial antibodies from Company S or Company C. Its exceptional sensitivity is evident in its ability to detect lower amounts of loading protein and produce stronger imaging signals with equal protein loading, highlighting its enhanced detection capabilities. These features make it an ideal choice for applications involving the detection of low-abundance proteins in qualitative analysis.

Tau ELISA Validation
Click to Zoom

Figure 1. GeneMedi’s GMLS-Tag001-Ab-1 anti-FLAG Mouse Monoclonal Antibody (M2 epitope) demonstrates superior performance in western blot and FACS assays.
A. Lane 1 contains cell lysate from untransfected 293T cells; lanes 2-5 contain 100 ng, 30 ng, 10 ng, and 3 ng of cell lysate from 293T cells transfected with a plasmid expressing engineered A protein-Flag. Immunoblot analysis was performed to detect A protein and Flag proteins.
B. Immunoblot analysis was conducted to detect Flag-tagged proteins. Lanes 1, 2, 4 and 6 contain cell lysates from 293T cells transfected with plasmids expressing proteins of different molecular weights. Lanes 3 and 5 contain control samples of 293T cell lysate without plasmid transfection.
C. A549 cells were transfected with a plasmid expressing engineered X protein-Flag. FACS was performed to detect Flag with control IgG, commercial Flag antibody and GMLS-Tag001-Ab-1.



GeneMedi’s HRP-conjugated anti-FLAG antibody demonstrates great performance in immunoblot analysis


GeneMedi’s HRP-conjugated anti-FLAG antibody demonstrates great performance and more convenient in western blot assays compared to GMLS-Tag001-Ab-1 anti-Flag Mouse Monoclonal Antibody (M2 epitope). Unlike unconjugated anti-FLAG antibodies, GeneMedi’s HRP-conjugated version enables direct detection, eliminating the need for secondary antibody incubation. This feature makes it an optimal choice for detecting FLAG-tagged proteins in qualitative analyses, offering researchers enhanced experimental efficiency and greater confidence in their results.

Tau ELISA Validation
Click to Zoom

Figure 2. GeneMedi’s HRP-conjugated anti-FLAG antibody exhibits excellent performance in Western blot assays.
A-B. Immunoblot analysis was conducted to detect Flag-tagged proteins with GeneMedi’s GMLS-Tag001-Ab-1 (A) or GMLS-Tag001-Ab-1-HRP (B). All images were acquired with a consistent exposure time of 1 second. Lane 1 contains 1000 ng of cell lysate from untransfected 293T cells (negative control). Lanes 2–4 contain 1000 ng, 300 ng, and 100 ng, respectively, of cell lysate from 293T cells transfected with a plasmid expressing engineered V-FLAG fusion protein (100 kDa).



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