GeneMedi Anti-FLAG Mouse Monoclonal Antibody (M2 epitope)



GeneMedi's Anti-FLAG Mouse Monoclonal Antibody (M2 epitope) is a high-quality, rigorously tested antibody specifically designed for accurate and efficient detection of FLAG-tagged proteins. Derived from mouse monoclonal sources, this antibody ensures high specificity and affinity towards FLAG epitope DYKDDDDK, a widely used tag in protein expression studies. GM’s anti-FLAG mab recognizes the same epitope as the M2 antibody.

Order information

Catalog No. Description Price(In USD) Qty (Quantity) Sum(In USD)
GMLS-Tag001-Ab-1 Anti-FLAG Mouse Monoclonal Antibody (M2 epitope);Size:1mg 756
GMLS-Tag001-Ab-1 Anti-FLAG Mouse Monoclonal Antibody (M2 epitope);Size:10mg 5292
GMLS-Tag001-Ab-1 Anti-FLAG Mouse Monoclonal Antibody (M2 epitope);Size:100mg 37044
GMLS-Tag001-Ab-1 Size≥100mg Inquiry
Shipping Cost: 760.00
Total:

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Description


Anti-FLAG Mouse Monoclonal Antibody (M2 epitope)

Cat No. GMLS-Tag001-Ab-1
Product Name Anti-FLAG Mouse Monoclonal Antibody (M2 epitope)
Target FLAG
Expression platform mammalian cell
Isotypes Mouse IgG
Bioactivity validation FLAG binding, Competitive immunoassay validation (Competitive ELISA) in FLAG level test.
Tag mFc
Products description Anti-FLAG mouse monoclonal antibody (M2 Epitope) monoclonal antibody is expressed from mammalian cell.
Species Mouse
Purity Purity: ≥95% （SDS-PAGE）
Application Western Blotting, Immunoprecipitation (IP), Immunofluorescence (IF), Flow Cytometry, and Immunohistochemistry (IHC) for accurate and efficient detection of FLAG-tagged proteins.
Formulation & Reconstitution Lyophilized from GM's Protein Stability Buffer2 (PSB2,Confidential Ingredients) or PBS (pH7.4);
For PSB2, reconstituted with 0.9% sodium chloride;
For PBS, reconstituted with ddH2O.
Storage Store at -20℃ to -80℃ under sterile conditions. Avoid repeated freeze-thaw cycles.


Key Applications

  1. Western Blotting: Ideal for detecting FLAG-tagged proteins in SDS-PAGE-separated samples.

  2. Immunoprecipitation (IP): Excellently suited for isolating FLAG-tagged proteins from complex mixtures, facilitating downstream analysis.

  3. Immunofluorescence (IF): Useful in localizing FLAG-tagged proteins within cells, aiding in cellular biology studies.

  4. Flow Cytometry: Enables the identification and analysis of FLAG-tagged proteins in suspension cells.

  5. Immunohistochemistry (IHC): Applicable for detecting and visualizing FLAG-tagged proteins in tissue sections.



Why Choose GeneMedi's Product?

  1. Consistency and Reliability: Each batch undergoes stringent quality control tests, ensuring consistent performance.

  2. High Sensitivity: Optimized to detect even low levels of FLAG-tagged proteins, ensuring no target goes unnoticed.

  3. Reduced Background Noise: Minimizes non-specific bindings, leading to clear and interpretable results.

  4. Versatility: Compatible with a variety of sample types and research applications.

  5. Support and Resources: GeneMedi provides comprehensive technical support and detailed product documentation to assist researchers in their experiments.


Validation Data


GeneMedi's Anti-FLAG antibody demonstrates superior performance in immunoblot and FACS analysis



GeneMedi’s GMLS-Tag001-Ab-1 anti-FLAG Mouse Monoclonal Antibody (M2 epitope) demonstrates superior performance in western blot and FACS assays compared to other commercial antibodies from Company S or Company C. Its exceptional sensitivity is evident in its ability to detect lower amounts of loading protein and produce stronger imaging signals with equal protein loading, highlighting its enhanced detection capabilities. These features make it an ideal choice for applications involving the detection of low-abundance proteins in qualitative analysis.

Tau ELISA Validation
Click to Zoom

Figure 1. GeneMedi’s GMLS-Tag001-Ab-1 anti-FLAG Mouse Monoclonal Antibody (M2 epitope) demonstrates superior performance in western blot and FACS assays.
A. Lane 1 contains cell lysate from untransfected 293T cells; lanes 2-5 contain 100 ng, 30 ng, 10 ng, and 3 ng of cell lysate from 293T cells transfected with a plasmid expressing engineered A protein-Flag. Immunoblot analysis was performed to detect A protein and Flag proteins.
B. Immunoblot analysis was conducted to detect Flag-tagged proteins. Lanes 1, 2, 4 and 6 contain cell lysates from 293T cells transfected with plasmids expressing proteins of different molecular weights. Lanes 3 and 5 contain control samples of 293T cell lysate without plasmid transfection.
C. A549 cells were transfected with a plasmid expressing engineered X protein-Flag. FACS was performed to detect Flag with control IgG, commercial Flag antibody and GMLS-Tag001-Ab-1.



Click to get more Data / Case study about the product.