Advantages and disadvantages of chemiluminescence immunoassay
Chemiluminescence immunoassay has unique characteristics of high sensitivity, high specificity, speed, accuracy, specificity, and automation.
It plays an important role in clinical testing, drug analysis, environmental monitoring and other fields. Especially the determination of various hormones, tumor markers, drug concentration and other trace biologically active substances.
In practical analysis, it is necessary to detect complex samples with large amounts and low abundance, so researchers have followed the trend to develop rapid, high-throughput and sensitive CLIA.
The time for measuring the optical signal of each sample is no more than a few seconds, and it can be completed within 9-60 minutes from sample addition to analysis result.
Additionally, CLIA has a wide dynamic range, which means that it can accurately measure a broad range of concentrations in a single assay. Another advantage of CLIA is that it is a homogeneous assay, meaning that it does not require any separation or washing steps, making it a fast and efficient assay.
However, CLIA also has some disadvantages. One limitation of CLIA is that it requires specialized equipment and reagents, which can be costly and may not be available in all laboratories. Another limitation to consider is the lot-to-lot variability, an issue for all assays.
Lot-to-lot differences in the reagents can be due to variability of raw materials used in the formulation of the reagents. This can impact the quality of the assay and must be carefully analyzed. On the other hand, It is important to check lot-to-lot differences in the calibrations and controls because response of certain reagents may change.
Although the development of a chemiluminescent immunoassay requires the optimization of several key parameters, with the help of solid expertise of researchers in the field, improved development of chemiluminescent immunoassays can be developed.
Additionally, CLIA may be subject to interference from matrix effects, such as high levels of lipids or proteins in a sample, which can lead to false-positive or false-negative results.
The other disadvantages are:
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(1) the light-emitting process is short;
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(2) High background;
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(3) High instrument failure rate;
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(4) The detection accuracy is not high
Guidence of GeneMedi's protocol / procedure for the diagnostics application: 
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