Pre-made biosensors & Organelle Bioprobe in adenovirus & AAV vector
Pre-made biosensors & Organelle Bioprobe in adenovirus & AAV vector:
| Cat No. | Name of Biosensors (BioProbes) | Biosensors/Bioprobes Description |
| GM-Probe-Adsensor-01 | Adv-actin indicator(mTurquoise reporter) | Actin cytoskeletal localization |
| GM-Probe-Adsensor-02 | Adv-GCaMP6m(medium variant calcium indicator) | GcaMP6 is a super-sensitive fluorescent protein, which is widely used to reflect the changes of intracellular free Ca2+ concentration. GcaMP6 fluorescent protein can be used as a hypersensitive Ca2+ probe to study the effects of external factors on Ca2+ in living cells under physiological and pathological conditions.GCaMP6m is a genetically encoded fluorescent Ca[2+] indicator that shows high sensitivity and medium decay kinetics. It consists of the calmodulin-binding peptide M13, a circularly permuted green fluorescent protein and calmodulin. |
| GM-Probe-Adsensor-03 | Adv-GCaMP6s(slow variant calcium indicator) | |
| GM-Probe-Adsensor-04 | Adv-GCaMP6f(fast variant calcium indicator) | |
| GM-Probe-Adsensor-05 | Adv-Peroxisome indicator(mTurquoise reporter) | Peroxisome localization |
| GM-Probe-Adsensor-06 | Adv-lactate biosensor(Laconic fluorescent protein) | Laconic fluorescent protein can specifically and efficiently reflect the changes of lactate concentration in living cells, so as to reflect the influence of external factors on intracellular metabolism in real time. It can be used to study the metabolic rate of lactic acid in cells. |
| GM-Probe-Adsensor-07 | Adv-NES(nuclear export signal) indicator(mTurquoise reporter) | NES(nuclear export signal), which is for export from the cell nucleus to the cytoplasm localization |
| GM-Probe-Adsensor-08 | Adv-Mito(mitochondrial)indicator(mTurquoise reporter) | Mito (mitochondrial) localization |
| GM-Probe-Adsensor-09 | Adv-H2O2 biosensor(Laconic fluorescent protein) | HyPer(Hydrogen peroxide sensor) is the first fully genetically encoded fluorescent sensor capable of detecting intracellular hydrogen peroxide (H2O2), one of the main ROS generated by cells. HyPer demonstrates submicromolar affinity to hydrogen peroxide and is insensitive to other oxidants tested, such as superoxide, oxidized glutathione, nitric oxide, and peroxinitrite. HyPer does not cause artifactual ROS generation and can be used for detection of fast changes of H2O2 concentration in different cell compartments under various physiological and pathological conditions. |
| GM-Probe-Adsensor-10 | Adv-Tubulin indicator(mTurquoise reporter) | Tubulin cytoskeletal localization |
| GM-Probe-Adsensor-11 | Adv-ER(endoplasmic reticulum) indicator(mTurquoise reporter) | ER(endoplasmic reticulum) localization |
| GM-Probe-Adsensor-12 | Adv-Membrane voltage(membrane potential) biosensor(VSFP) | VSFP(voltagesensitive fluorescent protein) Butterfly fluorescent protein can accurately reflect the polarization, depolarization and other membrane potential changes of excitatory cells such as cardiomyocytes and neurons |
| GM-Probe-Adsensor-13 | Adv-High glucose concentration biosensor(Hglu) | FLIPglu fluorescent protein can specifically and efficiently reflect the changes of glucose concentration in living cells, so as to reflect the influence of external factors on intracellular metabolism in real time. It can be used to study intracellular glucose metabolism rate and transmembrane transport rate. |
| GM-Probe-Adsensor-14 | Adv-Low glucose concentration biosensor(Lglu) | |
| GM-Probe-Adsensor-15 | Adv-lysosome indicator(Lamp1-RFP fusion protein) | Lamp1(lysosome associated membrane protein 1) is fused to RFP. Used for localization to the lysosome. |
| GM-Probe-Adsensor-16 | Adv-Mg2+ biosensor(Mag6) | Mag6(Mag FRET fluorescence protein) can specifically and efficiently reflect the change of intracellular free Mg2+ concentration. Mg2+ is an important ion to maintain the stability of ATP and a catalyst for many proteins in cells to maintain enzyme activity. It regulates a lot of signal transduction processes. |
| GM-Probe-Adsensor-17 | Adv-ATP biosensor(ATeam1.03) | ATeam1.03,a FRET-based ATP Fluorescent Biosensor, is an Adenosine 5-Triphosphate indicator based on Epsilon subunit for Analytical Measurements |
| GM-Probe-Adsensor-18 | Adv-mitochondrial autophagy indicator(mt-mKeima) | Monomeric Keima (mKeima)， a coral-derived acid-stable fluorescent protein that emits different-colored signals at acidic and neutral pHs. The mitochondrial mKeima(mt-mKeima) can be shown to enter the lysosome mitochondria through the autophagy pathway, so as to directly reflect the degree of mitochondrial autophagy. |
| GM-Probe-Adsensor-19 | Adv-Mito(mitochondrial)indicator(DsRed reporter) | The mitochondrial targeting sequence from subunit VIII of human cytochrome c oxidase (Mito) is fused to the 5'-end of DsRed,which is designed for fluorescent labeling of mitochondria. |
| GM-Probe-AAVsensor-01 | AAV-actin indicator(mTurquoise reporter) | Actin cytoskeletal localization |
| GM-Probe-AAVsensor-02 | AAV-GCaMP6m(medium variant calcium indicator) | GcaMP6 is a super-sensitive fluorescent protein, which is widely used to reflect the changes of intracellular free Ca2+ concentration. GcaMP6 fluorescent protein can be used as a hypersensitive Ca2+ probe to study the effects of external factors on Ca2+ in living cells under physiological and pathological conditions.GCaMP6m is a genetically encoded fluorescent Ca[2+] indicator that shows high sensitivity and medium decay kinetics. It consists of the calmodulin-binding peptide M13, a circularly permuted green fluorescent protein and calmodulin. |
| GM-Probe-AAVsensor-03 | AAV-GCaMP6s(slow variant calcium indicator) | |
| GM-Probe-AAVsensor-04 | AAV-GCaMP6f(fast variant calcium indicator) | |
| GM-Probe-AAVsensor-05 | AAV-Peroxisome indicator(mTurquoise reporter) | Peroxisome localization |
| GM-Probe-AAVsensor-06 | AAV-lactate biosensor(Laconic fluorescent protein) | Laconic fluorescent protein can specifically and efficiently reflect the changes of lactate concentration in living cells, so as to reflect the influence of external factors on intracellular metabolism in real time. It can be used to study the metabolic rate of lactic acid in cells. |
| GM-Probe-AAVsensor-07 | AAV-NES(nuclear export signal) indicator(mTurquoise reporter) | NES(nuclear export signal), which is for export from the cell nucleus to the cytoplasm localization |
| GM-Probe-AAVsensor-08 | AAV-Mito(mitochondrial) indicator(mTurquoise reporter) | Mito (mitochondrial) localization |
| GM-Probe-AAVsensor-09 | AAV-H2O2 biosensor(Laconic fluorescent protein) | HyPer(Hydrogen peroxide sensor) is the first fully genetically encoded fluorescent sensor capable of detecting intracellular hydrogen peroxide (H2O2), one of the main ROS generated by cells. HyPer demonstrates submicromolar affinity to hydrogen peroxide and is insensitive to other oxidants tested, such as superoxide, oxidized glutathione, nitric oxide, and peroxinitrite. HyPer does not cause artifactual ROS generation and can be used for detection of fast changes of H2O2 concentration in different cell compartments under various physiological and pathological conditions. |
| GM-Probe-AAVsensor-10 | AAV-Tubulin indicator(mTurquoise reporter) | Tubulin cytoskeletal localization |
| GM-Probe-AAVsensor-11 | AAV-ER(endoplasmic reticulum) indicator(mTurquoise reporter) | ER(endoplasmic reticulum) localization |
| GM-Probe-AAVsensor-12 | AAV-Membrane voltage(membrane potential) biosensor(VSFP) | VSFP(voltagesensitive fluorescent protein) Butterfly fluorescent protein can accurately reflect the polarization, depolarization and other membrane potential changes of excitatory cells such as cardiomyocytes and neurons |
| GM-Probe-AAVsensor-13 | AAV-High glucose concentration biosensor(Hglu) | FLIPglu fluorescent protein can specifically and efficiently reflect the changes of glucose concentration in living cells, so as to reflect the influence of external factors on intracellular metabolism in real time. It can be used to study intracellular glucose metabolism rate and transmembrane transport rate. |
| GM-Probe-AAVsensor-14 | AAV-Low glucose concentration biosensor(Lglu) | |
| GM-Probe-AAVsensor-15 | AAV-lysosome indicator(Lamp1-RFP fusion protein) | Lamp1(lysosome associated membrane protein 1) is fused to RFP. Used for localization to the lysosome. |
| GM-Probe-AAVsensor-16 | AAV-Mg2+ biosensor(Mag6) | Mag6(Mag FRET fluorescence protein) can specifically and efficiently reflect the change of intracellular free Mg2+ concentration. Mg2+ is an important ion to maintain the stability of ATP and a catalyst for many proteins in cells to maintain enzyme activity. It regulates a lot of signal transduction processes. |
| GM-Probe-AAVsensor-17 | AAV-ATP biosensor(ATeam1.03) | ATeam1.03,a FRET-based ATP Fluorescent Biosensor, is an Adenosine 5-Triphosphate indicator based on Epsilon subunit for Analytical Measurements |
| GM-Probe-AAVsensor-18 | AAV-mitochondrial autophagy indicator(mt-mKeima) | Monomeric Keima (mKeima), a coral-derived acid-stable fluorescent protein that emits different-colored signals at acidic and neutral pHs. The mitochondrial mKeima(mt-mKeima) can be shown to enter the lysosome mitochondria through the autophagy pathway, so as to directly reflect the degree of mitochondrial autophagy. |
| GM-Probe-AAVsensor-19 | AAV-Mito(mitochondrial)indicator(DsRed reporter) | The mitochondrial targeting sequence from subunit VIII of human cytochrome c oxidase (Mito) is fused to the 5'-end of DsRed,which is designed for fluorescent labeling of mitochondria. |
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