Electron microscopy (EM), Molecular Methods - strategies used in diagnosis of animal infectious disease for animal health

Electron microscopy allows direct visualization of virus particles. Two EM techniques are commonly used in diagnostic laboratories: negative-stain EM and ultrathin-section EM. Negative-stain EM for detection of virus particles in a fluid matrix; ultrathin-section EM for detection of virus particles in fixed tissues or cells. Based on characteristic morphology and size of virus particles observed under EM, viruses can be assigned to appropriate family, e.g., coronavirus-like particles were observed in some feces during initial investigation of diarrheic cases caused by porcine delta-coronavirus. Although EM cannot identify viruses to the species level, identification to the family level can still facilitate next-step testing to achieve definite diagnosis. However, EM generally is less sensitive and needs presence of sufficient amount of virus (about105–6virions per milliliter) in examined specimens. In addition, EM requires expensive equipment and highly skilled microscopist.
Figure 4. Transmission Electron Microscopy of hantavirus virions[18].

Category

Genemedi provides diagnostic antibodies and antigens for the in vitro diagnosis of diseases from the Companion Animal, Cat/Feline, Dog/Canine, Rabbit, Bovines/Cattle, Ovines/Sheep, Caprine/Goat, Equine/Horse, Swine/Porcine/Pig, Avian/bird/poultry, Fish and so on.

Animal category

Bovines/Cattles

Ovines/Sheep

Caprine/Goat

Deer

Cat/Feline

Dog/Canine

Rabbit

Equine/Horse

Swine/Porcine/Pig

Avian/Bird/Poultry

Fish

Others

Disease category

Technical resource

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