NT-proBNP Antibodies & Antigens for Veterinary Diagnostics
NT-proBNP, which stands for N-terminal pro b-type natriuretic peptide, is one of the tools doctors use to diagnose the conditions as well as their severity. Derived from the heart mainly due to stress and ventricular volume increase, they signal heart strain and facilitate the choice and outcomes of the treatment.
Validation data of GeneMedi's products
- Figure 1-2: Immunoassay calibration curve for NT-proBNP
- Figure 3: Sensitivity detection of NT-proBNP
- Figure 4-5: Stability Test of NT-proBNP
These immunoassays have a sensitivity of 22 pg/mL for measuring recombinant NT-proBNP. Figures 1 and 2 display the suggested pairing calibration curves. Conversion of 1NT-proBNP concentration: 1 pmol/L = 1÷10.545 pg/ml Please note that a variety of factors might affect how well an immunoassay test performs. These consist of the labeling procedure, the kind of label coupled to the detection antibody, and the detection platform. Thus, on our customers' immunoassay systems, other NT-proBNP antibody pairs with non-overlapping epitopes could perform better.
Figure 1-2. NT-proBNP immunoassay calibration curve
(left) Calibration curve for optimal paired detection
(right) Calibration curve and canine plasma sample dilution
comparison
Reagent type: two-step sandwich immunofluorescence detection,
streptavidin-coated
microplate
Capture antibody: 200ng/well, conjugated biotin
Capture antibody: 200ng/well, labeled europium chelate
Antigen: Canine recombinant NT-proBNP
(Cat.#GMP-CAN-NT-proBNP-Ag01)
Sample volume: 50μl
Incubation time: 40 minutes at room temperature
These immunoassays have a sensitivity of 22 pg/ml 1 for measuring recombinant NT-proBNP. Figures 1 and 2 display the suggested pairing calibration curves. Conversion of 1 NT-proBNP concentration: 1 pmol/L = 1÷10.545 pg/ml Please be aware that a variety of factors might affect how well an immunoassay test performs. These consist of the labeling procedure, the kind of label coupled to the detection antibody, and the detection platform. Thus, on our customers' immunoassay systems, other NT-proBNP antibody pairs with non-overlapping epitopes could perform better.
Figure 3. NT-proBNP concentration in EDTA plasma samples from healthy dogs and dogs with heart disease.
Reagent type: two-step sandwich immunofluorescence detection,
streptavidin-coated
microplate
Capture antibody: GMP-CAN-NT-proBNP-Ab01, 1μg/well
Capture antibody: GMP-CAN-NT-proBNP-Ab02, 200ng/well, labeled
europium
chelate
Calibrator: Canine recombinant NT-proBNP (Cat.#
GMP-CAN-NT-proBNP-Ag01)
Sample volume: 50μl
Incubation time: 40 minutes at room temperature
Our antibodies were tested for their ability to detect endogenous canine NT-proBNP during sample storage. EDTA plasma from dogs with heart disease was incubated at two temperatures: +4°C and +20°C. At +4°C, NT-proBNP remains stable for at least 72 hours (95%–105% of initial immunoreactivity, Figure 4A). Simultaneously, at +20°C, about 98% of the initial immunoreactivity is detectable after 24 hours (Figure 4B). Using our recommended antibody pairings, plasma can be stored at +4°C for at least 72 hours with minimal loss of immunoreactivity. However, at room temperature, there is a slight weakening of the signal during the first 24 hours. Note that individual plasma or serum samples may vary.
Figure 4-5. Stability of endogenous canine NT-proBNP in EDTA plasma
(left) Immunoreactivity of NT-proBNP in plasma samples incubated for
24, 48 and
72 hours at
+4°C.
(right) Immunoreactivity of NT-proBNP in plasma samples incubated
for 4, 8, 24
and 48 hours
at +20°C.
Collect protease
inhibitor-free EDTA plasma; samples are centrifuged, separated, and
stored at
-70°C
before use. EDTA plasma is stored
at +4°C or +20°C with the addition of 0.1% NaN3 to prevent bacterial
growth.
After
incubation, samples were stored at
-70°C before measurement. The NT-proBNP concentration in plasma was
9 ng/ml
(determined
by GMP-CAN-NT-proBNP-Ab01 and
GMP-CAN-NT-proBNP-Ab02 immunoassays). Detection using
GMP-CAN-NT-proBNP-Ab01 and
GMP-CAN-NT-proBNP-Ab05 as capture
antibodies and GMP-CAN-NT-proBNP-Ab06 and GMP-CAN-NT-proBNP-Ab02 as
detection
antibodies
respectively The system
results are depicted in Figure 3.
What is NT-proBNP? Decoding the Heart Health Biomarker
NT-proBNP stands for natriuretic peptide type B, N-terminal pro b-type natriuretic peptide is one of the biomarkers typically employed in the veterinary medicine to measure the health of the animals hearts. Contrary to BNP, NT-proBNP is secreted in the bloodstream as a result of alterations in pressure in the heart’s ventricles. : raised NT-proBNP could thus be suggestive of heart ailment like cardiomyopathy or congestive heart failure in cats and dogs.
This marker is thus of great help in identifying, staging and naming of heart diseases and in management of the diseases in animals. It is easily availed in the diagnosis of heart disorders and hence widely used as a screening test in veterinary cardiology due to its simplicity and minimal invasiveness in most cases, and common methods of sample collection involve blood sampling or a slow IV infusion into the patient.
Structure of NT-proBNP
Diseases Detected by NT-proBNP: Insights into Veterinary Cardiology
The following table indicates the comparison of NT-proBNP values in relation to heart disease in distinct animals showing normal and disease ranges and the approaches used in quantification.
Animals | Normal Range | Possible Diseases Listed (Detail) | Specimens/Biofluids | Measurement Method |
---|---|---|---|---|
Cat | <100 pmol/L | HCM (Hypertrophic Cardiomyopathy);RCM (Restrictive Cardiomyopathy); DCM (Dilated Cardiomyopathy) ; Congestive Heart Failure;Arterial Thromboembolism; Systemic Hypertension | Blood Plasma/Serum | ELISA, Chemiluminescence |
Dog | <900 pmol/L | DCM; Valvular Heart Disease;Congestive Heart Failure;Chronic Valvular Disease;Pulmonary Hypertension;Cardiac Arrhythmias | Blood Plasma/Serum | ELISA, Chemiluminescence |
Horse | - | Atrial Fibrillation; Equine Metabolic Syndrome-related cardiac changes;Congestive Heart Failure | Blood Plasma/Serum | ELISA, Chemiluminescence |
Cattle | - | Limited data available, but potential use in detecting heart failure or stress due to metabolic syndromes in dairy cows | Blood Plasma/Serum | ELISA, Chemiluminescence |
Note:
"HCM" stands for Hypertrophic Cardiomyopathy, a common heart disease in
cats
characterized by
thickened heart walls."DCM" is Dilated Cardiomyopathy, observed in dogs,
characterized by an
enlarged heart with poor contraction.The "Normal Range" values provided
are
generalized;
specific values can vary based on the testing method, equipment used,
and individual
patient
factors. It's important to consult veterinary diagnostic reference
materials or
laboratories
for precise normal ranges.In cases where data is not available ("-"), it
indicates
that the
use of NT-proBNP for those specific animals or diseases is either not
common or not
well-documented in available literature.
Product list of GeneMedi's BNP products
Catalog No. | Products Name | Detected Species | Disease/Condition | Products Type | Products Information |
GMP-FEL-BNP-Ag | Feline B-type natriuretic peptide (BNP) antigen | Cat/Feline | Cardiovascular Disease | protein | Details |
GMP-FEL-BNP-Ab | Anti-Feline B-type natriuretic peptide (BNP) antibody | Cat/Feline | Cardiovascular Disease | antibody(Fc/mFc) | Details |
GMP-CAN-BNP-Ag | Canine B-type natriuretic peptide (BNP) antigen | Dog/Canine | Cardiovascular Disease | protein | Details |
GMP-CAN-BNP-Ab | Anti-Canine B-type natriuretic peptide (BNP) antibody | Dog/Canine | Cardiovascular Disease | antibody(Fc/mFc) | Details |
GMP-BOV-BNP-Ag | Bovine B-type natriuretic peptide (BNP) antigen | Bovines/Cattle | Cardiovascular Disease | protein | Details |
GMP-BOV-BNP-Ab | Anti-Bovine B-type natriuretic peptide (BNP) antibody | Bovines/Cattle | Cardiovascular Disease | antibody(Fc/mFc) | Details |
GMP-EQU-BNP-Ag | Equine B-type natriuretic peptide (BNP) antigen | Equine/Horse | Cardiovascular Disease | protein | Details |
GMP-EQU-BNP-Ab | Anti-Equine B-type natriuretic peptide (BNP) antibody | Equine/Horse | Cardiovascular Disease | antibody(Fc/mFc) | Details |
GMP-FEL-NT-proBNP-Ag | Feline NT-proBNP (N-terminal prohormone of brain natriuretic peptide) antigen | Cat/Feline | Heart failure, cardiac disease | peptide-(naked/BSA/OVA) | Details |
GMP-FEL-NT-proBNP-Ab | Anti-Feline NT-proBNP (N-terminal prohormone of brain natriuretic peptide) antibody | Cat/Feline | Heart failure, cardiac disease | antibody(Fc/mFc) | Details |
GMP-CAN-NT-proBNP-Ag | Canine NT-proBNP (N-terminal prohormone of brain natriuretic peptide) antigen | Dog/Canine | Heart failure, cardiac disease | peptide-(naked/BSA/OVA) | Details |
GMP-CAN-NT-proBNP-Ab | Anti-Canine NT-proBNP (N-terminal prohormone of brain natriuretic peptide) antibody | Dog/Canine | Heart failure, cardiac disease | antibody(Fc/mFc) | Details |
GMP-BOV-NT-proBNP-Ag | Bovine NT-proBNP (N-terminal prohormone of brain natriuretic peptide) antigen | Bovines/Cattle | Cardiac disease | peptide-(naked/BSA/OVA) | Details |
GMP-BOV-NT-proBNP-Ab | Anti-Bovine NT-proBNP (N-terminal prohormone of brain natriuretic peptide) antibody | Bovines/Cattle | Cardiac disease | antibody(Fc/mFc) | Details |
GMP-EQU-NT-proBNP-Ag | Equine NT-proBNP (N-terminal prohormone of brain natriuretic peptide) antigen | Equine/Horse | Cardiac disease | peptide-(naked/BSA/OVA) | Details |
GMP-EQU-NT-proBNP-Ab | Anti-Equine NT-proBNP (N-terminal prohormone of brain natriuretic peptide) antibody | Equine/Horse | Cardiac disease | antibody(Fc/mFc) | Details |
GMP-FEL-proBNP-Ag | Feline pro B-type natriuretic peptide (proBNP) antigen | Cat/Feline | intrinsic cardiac dysfunction |
peptide-(naked/BSA/OVA) | Details |
GMP-FEL-proBNP-Ab | Anti-Feline pro B-type natriuretic peptide (proBNP) antibody | Cat/Feline | intrinsic cardiac dysfunction |
antibody(Fc/mFc) | Details |
GMP-CAN-proBNP-Ag | Canine pro B-type natriuretic peptide (proBNP) antigen | Dog/Canine | intrinsic cardiac dysfunction |
peptide-(naked/BSA/OVA) | Details |
GMP-CAN-proBNP-Ab | Anti-Canine pro B-type natriuretic peptide (proBNP) antibody | Dog/Canine | intrinsic cardiac dysfunction |
antibody(Fc/mFc) | Details |
GMP-BOV-proBNP-Ag | Bovine pro B-type natriuretic peptide (proBNP) antigen | Bovines/Cattle | intrinsic cardiac dysfunction |
peptide-(naked/BSA/OVA) | Details |
GMP-BOV-proBNP-Ab | Anti-Bovine pro B-type natriuretic peptide (proBNP) antibody | Bovines/Cattle | intrinsic cardiac dysfunction |
antibody(Fc/mFc) | Details |
GMP-EQU-proBNP-Ag | Equine pro B-type natriuretic peptide (proBNP) antigen | Equine/Horse | intrinsic cardiac dysfunction |
peptide-(naked/BSA/OVA) | Details |
GMP-EQU-proBNP-Ab | Anti-Equine pro B-type natriuretic peptide (proBNP) antibody | Equine/Horse | intrinsic cardiac dysfunction |
antibody(Fc/mFc) | Details |
*Note: 1. The bioactivity of the antigen, as demonstrated through its binding capabilities and role as an immunogen in sandwich ELISA, lateral-flow tests, and other immunoassays, serves as a control material in disease-related assessments. 2. The paired antibody demonstrates bioactivity by binding to its recombinant antigen, validated in ELISA as both a capture and detection antibody for disease testing.