Immuno-Diagnostics Antibodies & Antigens for Veterinary Inflammation Test
Immuno-diagnostic antibodies and antigens for inflammation diagnosis in animals target specific markers like
CRP, SAA, and procalcitonin. These components are essential for developing assays that accurately detect and
quantify inflammatory responses, aiding in the early diagnosis and management of inflammatory diseases in
veterinary medicine, ensuring timely and effective treatment.
Validation data of GeneMedi's products
- Canine CRP
- SAA (Serum Amyloid A)
GeneMedi's anti-cCRP monoclonal antibodies achieve excellent performance in immunoassays.
GeneMedi's monoclonal antibodies targeting cCRP (canine C-reactive protein) deliver outstanding performance across different immunoassay systems, ensuring high sensitivity and reliability for accurate cCRP detection. These antibodies are designed to support consistent and precise results, making them ideal for various diagnostic and research applications.
Figure 1. GeneMedi’s GMP-CAN-CRP monoclonal antibodies exhibit superior performance in sandwich and direct ELISA assays.
A.Calibration curve for natural cCRP antigen in the sandwich immunoassay.
B.Canine CRP levels in serum samples from both inflammatory and healthy individuals were measured using antibody pairs in a sandwich immunoassay.
C-D. Sandwich immunoassay was performed to compare PC (phosphocholine) and GMP-CAN-CRP-Ab01 as the capture antibody in the presence (C) and absence (D) of Ca2+.
E.Presence of PC does not affect CRP detection in sandwich immunoassays.
F.Detection of natural cCRP on a direct ELISA platform in the presence of Ca2+ or EDTA using GMP-CAN-CRP-Ab06.
G.The optimal antibody pairs selected based on the immunoassay system.
GeneMedi's anti-cCRP polyclonal antibodies and recombinant cCRP are suitable for use in the sandwich immunoassay system.
GeneMedi supplies polyclonal antibodies that can be used for the development of cCRP immunoassay systems, as well as recombinant antigens suitable for the calibration of cCRP immunoassay reagents, both of which exhibit excellent performance in the sandwich immunoassay system.
Figure 2. GeneMedi’s anti-cCRP polyclonal antibodies and recombinant cCRP show superior performance in sandwich ELISA assays.
A.Calibration curves for recombinant cCRP using mAb-mAb (red) and pAb-mAb (green) antibody pairs in the sandwich immunoassay.
B.Detection results of serum cCRP from normal and systemically inflamed dogs using two antibody pairs in a sandwich immunoassay.
C.Calibration curves for natural and recombinant cCRP in a sandwich immunoassay.
D.Titration curves for recombinant and natural cCRP in a sandwich immunoassay using PC as the capture molecule.
GeneMedi’s anti-SAA monoclonal antibodies demonstrate superior performance in immunoassay.
GeneMedi’s monoclonal antibodies for SAA (Serum Amyloid A) offer high sensitivity for detecting SAA in cat, dog, and horse serum. Due to the high sequence homology among these species, the same antibody pairs can be used to develop versatile sandwich immunoassays. As a result, GeneMedi’s anti-SAA antibodies provide a valuable tool for accurately monitoring and diagnosing inflammatory conditions across multiple species.
Figure 1. GeneMedi’s monoclonal antibodies demonstrate high sensitivity and specificity for detecting SAA in both healthy and inflammatory cat plasma samples using a sandwich immunoassay.
A. Calibration curve for GMP-FEL-Serum-amyloid-A-Ag01 in mixed cat plasma in the sandwich immunoassay. The SAA concentration in mixed cat plasma was 145 μg/ml, and the sample was diluted 208 times prior to testing.
B-C. Dilution curves for recombinant cat SAA antigen and plasma samples using the prototype reagents in the sandwich immunoassay. (B) Healthy cat samples (H1, H2), with SAA concentrations of 0.67 μg/ml and 0.45 μg/ml, respectively. Plasma samples were diluted 45 times before testing. The initial concentration of GMP-FEL-Serum-amyloid-A-Ag01 was 65 ng/ml. (C) Postoperative cat samples (P1, P2), with SAA concentrations of 169 μg/ml and 220 μg/ml, respectively. Plasma samples were diluted 200 times before testing. The initial concentration of GMP-FEL-Serum-amyloid-A-Ag01 was 200 ng/ml.
D. SAA concentrations in healthy and postoperative inflammatory cats. Healthy cat samples were pre-diluted 100-3000 times, while inflammatory cat samples were pre-diluted 9000 times prior to testing.
Figure 2. GeneMedi’s anti-SAA monoclonal antibodies enable sensitive detection of SAA in dog, horse, and cat serum using a sandwich immunoassay.
A-B. Dilution curves of recombinant dog (A) and horse (B) SAA antigens in the sandwich immunoassay. GMP-CAN-Serum-amyloid-A-Ab01 was coated and blocked at 37°C for 1 hour, and incubated with GMP-CAN-Serum-amyloid-A-Ag01 and GMP-EQU-Serum-amyloid-A-Ag01 at 37°C for 1 hour.
C. The recommended antibody pairs.
D-E. Comparison of SAA detection results between diseased and healthy individuals in dogs (D) and horses (E) in the sandwich immunoassay. Healthy serum was pre-diluted 50 times, while diseased serum was pre-diluted 1500 times (horse) or 2500 times (dog), with the dilution buffer containing 1% casein and 0.05% Tween 20.
F-H. Testing results of SAA in cat (F), dog (G), and horse (H) serum samples in the sandwich immunoassay. GMP-FEL-Serum-amyloid-A-Ab10 was blocked for 1 hour (blocking solution containing 2.5% sodium caseinate). Dog serum was pre-diluted 2500 times, while cat and horse serum were pre-diluted 1500 times, using a Tris buffer containing 0.01% CHAPS.
What is Inflammation and how does it Manifest in Different Animals?
Inflammation is a fundamental physiological response to injury, infection, or irritation in animals, just as it is in humans. It serves as the body's mechanism to initiate the healing process, involving the immune system fighting off invading pathogens, repairing damaged tissues, and restoring normal function. The signs of inflammation can be characterized by five classic symptoms: pain, heat, redness, swelling, and loss of function at the affected site.
Animals Affected by Inflammation
Virtually all animals with an immune system can experience inflammation. This includes a wide range of animals, from domestic pets to wildlife:
Domestic Pets:
-
· Dogs and Cats: Commonly suffer from inflammation due to various causes, including infections, allergies, chronic diseases (such as arthritis), and injuries.
-
· Small Mammals: Rabbits, guinea pigs, and ferrets can also experience inflammation, often evidenced by skin conditions, digestive issues, or respiratory problems.
Livestock:
-
· Cattle, Sheep, and Goats: These animals can develop inflammatory conditions like mastitis (inflammation of the mammary gland), foot rot, or systemic infections.
-
· Horses: Prone to inflammation in the form of laminitis (inflammation of the tissues within the hoof), tendonitis, and colitis, among other conditions.
-
· Wildlife: Wild animals are equally susceptible to inflammation, which can be more difficult to diagnose and treat. Inflammation can arise from natural causes, such as predation injuries or infections, or from human-induced factors, like pollution or habitat destruction.
The Top 10 Inflammatory Biomarkers: Roles and Importance
This table outlines critical biomarkers like SAA, CRP, and NT-proBNP, their normal ranges, and implications in diseases for dogs and cats. It serves as a reference for veterinarians to assess inflammation, infection, and cardiac health, incorporating measurement methods and age considerations.
| Biomarker | Normal Range (Dogs) | Normal Range (Cats) | Implications in Disease | Specimens/Biofluids | Age Considerations | Measurement Method |
|---|---|---|---|---|---|---|
| SAA (Serum Amyloid A) | <100 µg/mL | <10 µg/mL | Acute inflammation, infection | Blood, Serum | Levels may increase with age | Immunoassay |
| CRP (C-Reactive Protein) | <10 mg/L | <10 mg/L | Inflammation, infection | Blood, Serum | Elderly pets may have higher baseline levels | Immunoassay |
| PCT (Procalcitonin) | Not commonly measured | Not commonly measured | Bacterial infection (less used in veterinary medicine) | Blood, Serum | Not well-established in pets | Immunoassay |
| NT-proBNP | <900 pg/mL | <100 pg/mL | Heart disease | Blood, Plasma | Increases with cardiac stress and age | Immunoassay |
| D-Dimer | 0-0.5 µg/mL | 0-0.5 µg/mL | Thrombosis, DIC | Blood, Plasma | No specific age correlation | Immunoassay |
| cTnI (Cardiac Troponin I) | <0.1 ng/mL | <0.1 ng/mL | Cardiac injury | Blood, Serum | Not significantly affected by age | Immunoassay |
| Albumin | 2.5-4.0 g/dL | 2.5-3.9 g/dL | Liver disease, chronic inflammation | Blood, Serum | Decreases in chronic disease | Biochemical assay |
| ALT (Alanine Aminotransferase) | 10-125 U/L | 10-100 U/L | Liver injury | Blood, Serum | Younger animals may have slightly higher levels due to growth | Biochemical assay |
| Creatinine | 0.6-1.2 mg/dL | 0.6-2.4 mg/dL | Kidney function | Blood, Serum | Older pets may have higher levels due to decreased kidney function | Biochemical assay |
| Glucose | 70-150 mg/dL | 70-150 mg/dL | Diabetes, stress response | Blood, Serum | Puppies/kittens may have lower baseline levels; stress can elevate | Biochemical assay |
Diagnosing and Treating Inflammation in Animals
Inflammation is diagnosed based on clinical signs, medical history, and diagnostic tests, including blood tests to identify markers of inflammation (e.g., C-reactive protein) and imaging studies to assess the affected areas. Treatment varies with the underlying cause but generally involves:
-
Anti-inflammatory Medications: Nonsteroidal anti-inflammatory drugs (NSAIDs) or corticosteroids to reduce inflammation and pain.
-
Antibiotics or Antifungal Medications: If the inflammation is due to an infection.
-
Dietary Management and Supplements: Especially for chronic inflammatory conditions like arthritis, where omega-3 fatty acids may help reduce inflammation.
-
Surgery: In cases where physical removal of the cause of inflammation is necessary (e.g., abscess drainage, tumor removal).
Preventive care, including regular veterinary check-ups, vaccinations, and maintaining a healthy lifestyle, can help minimize the risk of inflammatory diseases in animals. Early detection and treatment are key to effectively managing inflammation and preventing its progression into more serious conditions.
Product list of GeneMedi's products for Veterinary Inflammation Test
| Catalog No. | Products Name | Specific Host | Products Information |
| GMP-BOV-Serum-amyloid-A-Ag | Bovine Serum amyloid A | Bovines/Cattle | Details |
| GMP-BOV-Serum-amyloid-A-Ab | Anti-bovine SAA protein monoclonal antibody (mAb) | Bovines/Cattle | Details |
| GMP-CAN-Serum-amyloid-A-Ab | Anti-Canine Serum amyloid A monoclonal antibody (mAb) | Dog/Canine | Details |
| GMP-CAN-Serum-amyloid-A-Ag | Serum amyloid A | Dog/Canine | Details |
| GMP-EQU-Serum-amyloid-A-Ab | Anti-Equine SAA monoclonal antibody (mAb) | Equine/Horse | Details |
| GMP-EQU-Serum-amyloid-A-Ag | Serum amyloid A | Equine/Horse | Details |
| GMP-FEL-Serum-amyloid-A-Ab | Anti-Feline Serum amyloid A monoclonal antibody (mAb) | Cat/Feline | Details |
| GMP-FEL-Serum-amyloid-A-Ag | Serum amyloid A | Cat/Feline | Details |
| GMP-CAN-PCT-Ag | PCT | Dog/Canine | Details |
| GMP-CAN-PCT-Ab | Anti-Canine PCT monoclonal antibody (mAb) | Dog/Canine | Details |
| GMP-EQU-PCT-Ag | PCT | Equine/Horse | Details |
| GMP-EQU-PCT-Ab | Anti-Equine PCT monoclonal antibody (mAb) | Equine/Horse | Details |
| GMP-FEL-PCT-Ag | PCT | Cat/Feline | Details |
| GMP-FEL-PCT-Ab | Anti-Feline PCT monoclonal antibody (mAb) | Cat/Feline | Details |
| GMP-BOV-CRP-Ag | C-Reactive Protein | Bovines/Cattle | Details |
| GMP-BOV-CRP-Ab | Anti-Bovine CRP monoclonal antibody (mAb) | Bovines/Cattle | Details |
| GMP-CAN-CRP-Ag | C-Reactive Protein | Dog/Canine | Details |
| GMP-CAN-CRP-Ab | Anti-Canine C-Reactive Protein (CRP) monoclonal antibody (mAb) | Dog/Canine | Details |
| GMP-EQU-CRP-Ag | C-Reactive Protein | Equine/Horse | Details |
| GMP-EQU-CRP-Ab | Anti-Equine CRP monoclonal antibody (mAb) | Equine/Horse | Details |
| GMP-FEL-CRP-Ag | C-Reactive Protein | Cat/Feline | Details |
| GMP-FEL-CRP-Ab | Anti-Feline CRP monoclonal antibody (mAb) | Cat/Feline | Details |
Facebook
LinkedIn
Twitter
