Immuno-Diagnostics Antibodies & Antigens for Veterinary Cardiovascular Diseases Test

Therefore, the assessment of cardiovascular health depends on biomarkers such as cTnI and NT-proBNP. Specific cardiac Troponin I points to cardiac muscle damage, whilst NT-proBNP tests the pressure on the heart due to heart failure. Higher amounts of these biomarkers help to identify acute cardiac events and, thereby, manage the further process effectively.





Validation data of GeneMedi's products

  • Figure 1-2: Immunoassay calibration curve for NT-proBNP
  • Figure 3: Sensitivity detection of NT-proBNP
  • Figure 4-5: Stability Test of NT-proBNP
proBNP Validation

These immunoassays have a sensitivity of 22 pg/mL for measuring recombinant NT-proBNP. Figures 1 and 2 display the suggested pairing calibration curves. Conversion of 1NT-proBNP concentration: 1 pmol/L = 1÷10.545 pg/ml Please note that a variety of factors might affect how well an immunoassay test performs. These consist of the labeling procedure, the kind of label coupled to the detection antibody, and the detection platform. Thus, on our customers' immunoassay systems, other NT-proBNP antibody pairs with non-overlapping epitopes could perform better.

Figure 1-2. NT-proBNP immunoassay calibration curve

(left) Calibration curve for optimal paired detection
(right) Calibration curve and canine plasma sample dilution comparison

Reagent type: two-step sandwich immunofluorescence detection, streptavidin-coated microplate
Capture antibody: 200ng/well, conjugated biotin
Capture antibody: 200ng/well, labeled europium chelate
Antigen: Canine recombinant NT-proBNP (Cat.#GMP-CAN-NT-proBNP-Ag01)
Sample volume: 50μl
Incubation time: 40 minutes at room temperature



proBNP Validation

These immunoassays have a sensitivity of 22 pg/ml 1 for measuring recombinant NT-proBNP. Figures 1 and 2 display the suggested pairing calibration curves. Conversion of 1 NT-proBNP concentration: 1 pmol/L = 1÷10.545 pg/ml Please be aware that a variety of factors might affect how well an immunoassay test performs. These consist of the labeling procedure, the kind of label coupled to the detection antibody, and the detection platform. Thus, on our customers' immunoassay systems, other NT-proBNP antibody pairs with non-overlapping epitopes could perform better.

Figure 3. NT-proBNP concentration in EDTA plasma samples from healthy dogs and dogs with heart disease.

Reagent type: two-step sandwich immunofluorescence detection, streptavidin-coated microplate
Capture antibody: GMP-CAN-NT-proBNP-Ab01, 1μg/well
Capture antibody: GMP-CAN-NT-proBNP-Ab02, 200ng/well, labeled europium chelate
Calibrator: Canine recombinant NT-proBNP (Cat.# GMP-CAN-NT-proBNP-Ag01)
Sample volume: 50μl
Incubation time: 40 minutes at room temperature

proBNP Validation

Our antibodies were tested for their ability to detect endogenous canine NT-proBNP during sample storage. EDTA plasma from dogs with heart disease was incubated at two temperatures: +4°C and +20°C. At +4°C, NT-proBNP remains stable for at least 72 hours (95%–105% of initial immunoreactivity, Figure 4A). Simultaneously, at +20°C, about 98% of the initial immunoreactivity is detectable after 24 hours (Figure 4B). Using our recommended antibody pairings, plasma can be stored at +4°C for at least 72 hours with minimal loss of immunoreactivity. However, at room temperature, there is a slight weakening of the signal during the first 24 hours. Note that individual plasma or serum samples may vary.

Figure 4-5. Stability of endogenous canine NT-proBNP in EDTA plasma

(left) Immunoreactivity of NT-proBNP in plasma samples incubated for 24, 48 and 72 hours at +4°C.
(right) Immunoreactivity of NT-proBNP in plasma samples incubated for 4, 8, 24 and 48 hours at +20°C.

Collect protease inhibitor-free EDTA plasma; samples are centrifuged, separated, and stored at -70°C before use. EDTA plasma is stored at +4°C or +20°C with the addition of 0.1% NaN3 to prevent bacterial growth. After incubation, samples were stored at -70°C before measurement. The NT-proBNP concentration in plasma was 9 ng/ml (determined by GMP-CAN-NT-proBNP-Ab01 and GMP-CAN-NT-proBNP-Ab02 immunoassays). Detection using GMP-CAN-NT-proBNP-Ab01 and GMP-CAN-NT-proBNP-Ab05 as capture antibodies and GMP-CAN-NT-proBNP-Ab06 and GMP-CAN-NT-proBNP-Ab02 as detection antibodies respectively The system results are depicted in Figure 3.



Identifying Signs of Cardiovascular Diseases in Pets

Cardiovascular diseases is a class of diseases affecting the heart and blood vessels; it consists of coronary artery diseases, heart failure, congenital heart diseases, peripheral arterial diseases, and stroke. These diseases are further accompanied by different pathological changes of various forms including the formation of the plaques in the coronary arteries (atherosclerosis) which may cause heart attacks or high blood pressure that could progress to heart failure. Hence, biomarkers are instrumental in the early identification and subsequently the tracking of CVDs.

Product list of GeneMedi's products for Veterinary Cardiovascular Test products

Catalog No. Products Name Detected Species Disease/Condition Products Type Products Information
GMP-FEL-NT-proBNP-Ag Feline NT-proBNP (N-terminal prohormone of brain natriuretic peptide) antigen Cat/Feline Heart failure, cardiac disease peptide-(naked/BSA/OVA) Details
GMP-FEL-NT-proBNP-Ab Anti-Feline NT-proBNP (N-terminal prohormone of brain natriuretic peptide) antibody Cat/Feline Heart failure, cardiac disease antibody(Fc/mFc) Details
GMP-CAN-NT-proBNP-Ag Canine NT-proBNP (N-terminal prohormone of brain natriuretic peptide) antigen Dog/Canine Heart failure, cardiac disease peptide-(naked/BSA/OVA) Details
GMP-CAN-NT-proBNP-Ab Anti-Canine NT-proBNP (N-terminal prohormone of brain natriuretic peptide) antibody Dog/Canine Heart failure, cardiac disease antibody(Fc/mFc) Details
GMP-EQU-NT-proBNP-Ag Equine NT-proBNP (N-terminal prohormone of brain natriuretic peptide) antigen Equine/Horse Cardiac disease peptide-(naked/BSA/OVA) Details
GMP-EQU-NT-proBNP-Ab Anti-Equine NT-proBNP (N-terminal prohormone of brain natriuretic peptide) antibody Equine/Horse Cardiac disease antibody(Fc/mFc) Details
GMP-BOV-NT-proBNP-Ag Bovine NT-proBNP (N-terminal prohormone of brain natriuretic peptide) antigen Bovines/Cattle Cardiac disease peptide-(naked/BSA/OVA) Details
GMP-BOV-NT-proBNP-Ab Anti-Bovine NT-proBNP (N-terminal prohormone of brain natriuretic peptide) antibody Bovines/Cattle Cardiac disease antibody(Fc/mFc) Details
GMP-FEL-cTnI-Ag Feline Cardiac troponin I (cTnI) antigen Cat/Feline Myocardial injury, heart disease protein Details
GMP-FEL-cTnI-Ab Anti-Feline Cardiac troponin I (cTnI) antibody Cat/Feline Myocardial injury, heart disease antibody(Fc/mFc) Details
GMP-CAN-cTnI-Ag Canine Cardiac troponin I (cTnI) antigen Dog/Canine Myocardial injury, heart disease protein Details
GMP-CAN-cTnI-Ab Anti-Canine Cardiac troponin I (cTnI) antibody Dog/Canine Myocardial injury, heart disease antibody(Fc/mFc) Details
GMP-EQU-cTnI-Ag Equine Cardiac troponin I (cTnI) antigen Equine/Horse Myocardial injury, heart disease protein Details
GMP-EQU-cTnI-Ab Anti-Equine Cardiac troponin I (cTnI) antibody Equine/Horse Myocardial injury, heart disease antibody(Fc/mFc) Details
GMP-BOV-cTnI-Ag Bovine Cardiac troponin I (cTnI) antigen Bovines/Cattle myocardial infarction protein Details
GMP-BOV-cTnI-Ab Anti-Bovine Cardiac troponin I (cTnI) antibody Bovines/Cattle myocardial infarction antibody(Fc/mFc) Details
GMP-FEL-cTnT-Ag Feline Cardiac troponin T (cTnT) antigen Cat/Feline hypertrophic cardiomyopathy, restrictive and dilated cardiomyopathy. protein Details
GMP-FEL-cTnT-Ab Anti-Feline Cardiac troponin T (cTnT) antibody Cat/Feline hypertrophic cardiomyopathy, restrictive and dilated cardiomyopathy. antibody(Fc/mFc) Details
GMP-CAN-cTnT-Ag Canine Cardiac troponin T (cTnT) antigen Dog/Canine hypertrophic cardiomyopathy, restrictive and dilated cardiomyopathy. protein Details
GMP-CAN-cTnT-Ab Anti-Canine Cardiac troponin T (cTnT) antibody Dog/Canine hypertrophic cardiomyopathy, restrictive and dilated cardiomyopathy. antibody(Fc/mFc) Details
GMP-EQU-cTnT-Ag Equine Cardiac troponin T (cTnT) antigen Equine/Horse hypertrophic cardiomyopathy, restrictive and dilated cardiomyopathy. protein Details
GMP-EQU-cTnT-Ab Anti-Equine Cardiac troponin T (cTnT) antibody Equine/Horse hypertrophic cardiomyopathy, restrictive and dilated cardiomyopathy. antibody(Fc/mFc) Details
GMP-BOV-cTnT-Ag Bovine Cardiac troponin T (cTnT) antigen Bovines/Cattle hypertrophic cardiomyopathy, restrictive and dilated cardiomyopathy. protein Details
GMP-BOV-cTnT-Ab Anti-Bovine Cardiac troponin T (cTnT) antibody Bovines/Cattle hypertrophic cardiomyopathy, restrictive and dilated cardiomyopathy. antibody(Fc/mFc) Details
GMP-FEL-sST2-Ag Feline sST2 antigen Cat/Feline chronic and acute heart failure protein Details
GMP-FEL-sST2-Ab Anti-Feline sST2 antibody Cat/Feline chronic and acute heart failure antibody(Fc/mFc) Details
GMP-CAN-sST2-Ag Canine sST2 antigen Dog/Canine chronic and acute heart failure protein Details
GMP-CAN-sST2-Ab Anti-Canine sST2 antibody Dog/Canine chronic and acute heart failure antibody(Fc/mFc) Details
GMP-EQU-sST2-Ag Equine sST2 antigen Equine/Horse chronic and acute heart failure protein Details
GMP-EQU-sST2-Ab Anti-Equine sST2 antibody Equine/Horse chronic and acute heart failure antibody(Fc/mFc) Details
GMP-BOV-sST2-Ag Bovine sST2 antigen Bovines/Cattle chronic and acute heart failure protein Details
GMP-BOV-sST2-Ab Anti-Bovine sST2 antibody Bovines/Cattle chronic and acute heart failure antibody(Fc/mFc) Details
GMP-FEL-proBNP-Ag Feline pro B-type natriuretic peptide (proBNP) antigen Cat/Feline intrinsic cardiac
dysfunction
peptide-(naked/BSA/OVA) Details
GMP-FEL-proBNP-Ab Anti-Feline pro B-type natriuretic peptide (proBNP) antibody Cat/Feline intrinsic cardiac
dysfunction
antibody(Fc/mFc) Details
GMP-CAN-proBNP-Ag Canine pro B-type natriuretic peptide (proBNP) antigen Dog/Canine intrinsic cardiac
dysfunction
peptide-(naked/BSA/OVA) Details
GMP-CAN-proBNP-Ab Anti-Canine pro B-type natriuretic peptide (proBNP) antibody Dog/Canine intrinsic cardiac
dysfunction
antibody(Fc/mFc) Details
GMP-EQU-proBNP-Ag Equine pro B-type natriuretic peptide (proBNP) antigen Equine/Horse intrinsic cardiac
dysfunction
peptide-(naked/BSA/OVA) Details
GMP-EQU-proBNP-Ab Anti-Equine pro B-type natriuretic peptide (proBNP) antibody Equine/Horse intrinsic cardiac
dysfunction
antibody(Fc/mFc) Details
GMP-BOV-proBNP-Ag Bovine pro B-type natriuretic peptide (proBNP) antigen Bovines/Cattle intrinsic cardiac
dysfunction
peptide-(naked/BSA/OVA) Details
GMP-BOV-proBNP-Ab Anti-Bovine pro B-type natriuretic peptide (proBNP) antibody Bovines/Cattle intrinsic cardiac
dysfunction
antibody(Fc/mFc) Details
GMP-FEL-BNP-Ag Feline B-type natriuretic peptide (BNP) antigen Cat/Feline Cardiovascular Disease protein Details
GMP-FEL-BNP-Ab Anti-Feline B-type natriuretic peptide (BNP) antibody Cat/Feline Cardiovascular Disease antibody(Fc/mFc) Details
GMP-CAN-BNP-Ag Canine B-type natriuretic peptide (BNP) antigen Dog/Canine Cardiovascular Disease protein Details
GMP-CAN-BNP-Ab Anti-Canine B-type natriuretic peptide (BNP) antibody Dog/Canine Cardiovascular Disease antibody(Fc/mFc) Details
GMP-EQU-BNP-Ag Equine B-type natriuretic peptide (BNP) antigen Equine/Horse Cardiovascular Disease protein Details
GMP-EQU-BNP-Ab Anti-Equine B-type natriuretic peptide (BNP) antibody Equine/Horse Cardiovascular Disease antibody(Fc/mFc) Details
GMP-BOV-BNP-Ag Bovine B-type natriuretic peptide (BNP) antigen Bovines/Cattle Cardiovascular Disease protein Details
GMP-BOV-BNP-Ab Anti-Bovine B-type natriuretic peptide (BNP) antibody Bovines/Cattle Cardiovascular Disease antibody(Fc/mFc) Details
GMP-FEL-ANP-Ag Feline atrial natriuretic peptide (ANP) antigen Cat/Feline hypertension, cardiac hypertrophy and heart failure protein Details
GMP-FEL-ANP-Ab Anti-Feline atrial natriuretic peptide (ANP) antibody Cat/Feline hypertension, cardiac hypertrophy and heart failure antibody(Fc/mFc) Details
GMP-CAN-ANP-Ag Canine atrial natriuretic peptide (ANP) antigen Dog/Canine hypertension, cardiac hypertrophy and heart failure protein Details
GMP-CAN-ANP-Ab Anti-Canine atrial natriuretic peptide (ANP) antibody Dog/Canine hypertension, cardiac hypertrophy and heart failure antibody(Fc/mFc) Details
GMP-EQU-ANP-Ag Equine atrial natriuretic peptide (ANP) antigen Equine/Horse hypertension, cardiac hypertrophy and heart failure protein Details
GMP-EQU-ANP-Ab Anti-Equine atrial natriuretic peptide (ANP) antibody Equine/Horse hypertension, cardiac hypertrophy and heart failure antibody(Fc/mFc) Details
GMP-BOV-ANP-Ag Bovine atrial natriuretic peptide (ANP) antigen Bovines/Cattle hypertension, cardiac hypertrophy and heart failure protein Details
GMP-BOV-ANP-Ab Anti-Bovine atrial natriuretic peptide (ANP) antibody Bovines/Cattle hypertension, cardiac hypertrophy and heart failure antibody(Fc/mFc) Details





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