Immuno-Diagnostics Antibodies & Antigens for Veterinary Cardiovascular Diseases Test
Therefore, the assessment of cardiovascular health depends on biomarkers such as cTnI and NT-proBNP. Specific cardiac Troponin I points to cardiac muscle damage, whilst NT-proBNP tests the pressure on the heart due to heart failure. Higher amounts of these biomarkers help to identify acute cardiac events and, thereby, manage the further process effectively.
Validation data of GeneMedi's products
- Canine NT-proBNP
- Feline cTnI
Immunoassay calibration curve for NT-proBNP
These immunoassays have a sensitivity of 22 pg/mL for measuring recombinant NT-proBNP. Figures 1 and 2 display the suggested pairing calibration curves. Conversion of 1NT-proBNP concentration: 1 pmol/L = 1÷10.545 pg/ml Please note that a variety of factors might affect how well an immunoassay test performs. These consist of the labeling procedure, the kind of label coupled to the detection antibody, and the detection platform. Thus, on our customers' immunoassay systems, other NT-proBNP antibody pairs with non-overlapping epitopes could perform better.
Figure 1-2. NT-proBNP immunoassay calibration curve
(left) Calibration curve for optimal paired detection
(right) Calibration curve and canine plasma sample dilution
comparison
Reagent type: two-step sandwich immunofluorescence detection,
streptavidin-coated
microplate
Capture antibody: 200ng/well, conjugated biotin
Capture antibody: 200ng/well, labeled europium chelate
Antigen: Canine recombinant NT-proBNP
(Cat.#GMP-CAN-NT-proBNP-Ag01)
Sample volume: 50μl
Incubation time: 40 minutes at room temperature
Sensitivity detection of NT-proBNP
These immunoassays have a sensitivity of 22 pg/ml 1 for measuring recombinant NT-proBNP. Figures 1 and 2 display the suggested pairing calibration curves. Conversion of 1 NT-proBNP concentration: 1 pmol/L = 1÷10.545 pg/ml Please be aware that a variety of factors might affect how well an immunoassay test performs. These consist of the labeling procedure, the kind of label coupled to the detection antibody, and the detection platform. Thus, on our customers' immunoassay systems, other NT-proBNP antibody pairs with non-overlapping epitopes could perform better.
Figure 3. NT-proBNP concentration in EDTA plasma samples from healthy dogs and dogs with heart disease.
Reagent type: two-step sandwich immunofluorescence detection,
streptavidin-coated
microplate
Capture antibody: GMP-CAN-NT-proBNP-Ab01, 1μg/well
Capture antibody: GMP-CAN-NT-proBNP-Ab02, 200ng/well, labeled
europium
chelate
Calibrator: Canine recombinant NT-proBNP (Cat.#
GMP-CAN-NT-proBNP-Ag01)
Sample volume: 50μl
Incubation time: 40 minutes at room temperature
Stability Test of NT-proBNP
Our antibodies were tested for their ability to detect endogenous canine NT-proBNP during sample storage. EDTA plasma from dogs with heart disease was incubated at two temperatures: +4°C and +20°C. At +4°C, NT-proBNP remains stable for at least 72 hours (95%–105% of initial immunoreactivity, Figure 4A). Simultaneously, at +20°C, about 98% of the initial immunoreactivity is detectable after 24 hours (Figure 4B). Using our recommended antibody pairings, plasma can be stored at +4°C for at least 72 hours with minimal loss of immunoreactivity. However, at room temperature, there is a slight weakening of the signal during the first 24 hours. Note that individual plasma or serum samples may vary.
Figure 4-5. Stability of endogenous canine NT-proBNP in EDTA plasma
(left) Immunoreactivity of NT-proBNP in plasma samples incubated for 24, 48 and 72 hours at +4°C.
(right) Immunoreactivity of NT-proBNP in plasma samples incubated for 4, 8, 24 and 48 hours at +20°C.
Collect protease inhibitor-free EDTA plasma; samples are centrifuged, separated, and stored at -70°C before use. EDTA plasma is stored at +4°C or +20°C with the addition of 0.1% NaN3 to prevent bacterial growth. After incubation, samples were stored at -70°C before measurement. The NT-proBNP concentration in plasma was 9 ng/ml (determined by GMP-CAN-NT-proBNP-Ab01 and GMP-CAN-NT-proBNP-Ab02 immunoassays). Detection using GMP-CAN-NT-proBNP-Ab01 and GMP-CAN-NT-proBNP-Ab05 as capture antibodies and GMP-CAN-NT-proBNP-Ab06 and GMP-CAN-NT-proBNP-Ab02 as detection antibodies respectively The system results are depicted in Figure 3.
GeneMedi's feline cTnI antibodies exhibit superior performance in sandwich immunoassays.
GeneMedi’s monoclonal antibodies for SAA (Serum Amyloid A) offer high sensitivity for detecting SAA in cat, dog, and horse serum. Due to the high sequence homology among these species, the same antibody pairs can be used to develop versatile sandwich immunoassays. As a result, GeneMedi’s anti-SAA antibodies provide a valuable tool for accurately monitoring and diagnosing inflammatory conditions across multiple species.
Figure 1. The performance of GeneMedi’s feline cTnI antibodies in sandwich ELISA assays.
A-B. Results of four different antibody pairs in sandwich ELISA assays.
C. Antibody pairs and their EC50 values.
Identifying Signs of Cardiovascular Diseases in Pets
Cardiovascular diseases is a class of diseases affecting the heart and blood vessels; it consists of coronary artery diseases, heart failure, congenital heart diseases, peripheral arterial diseases, and stroke. These diseases are further accompanied by different pathological changes of various forms including the formation of the plaques in the coronary arteries (atherosclerosis) which may cause heart attacks or high blood pressure that could progress to heart failure. Hence, biomarkers are instrumental in the early identification and subsequently the tracking of CVDs.
Product list of GeneMedi's products for Veterinary Cardiovascular Test products
| Catalog No. | Products Name | Detected Species | Disease/Condition | Products Type | Products Information |
| GMP-FEL-NT-proBNP-Ag | Feline NT-proBNP (N-terminal prohormone of brain natriuretic peptide) antigen | Cat/Feline | Heart failure, cardiac disease | peptide-(naked/BSA/OVA) | Details |
| GMP-FEL-NT-proBNP-Ab | Anti-Feline NT-proBNP (N-terminal prohormone of brain natriuretic peptide) antibody | Cat/Feline | Heart failure, cardiac disease | antibody(Fc/mFc) | Details |
| GMP-CAN-NT-proBNP-Ag | Canine NT-proBNP (N-terminal prohormone of brain natriuretic peptide) antigen | Dog/Canine | Heart failure, cardiac disease | peptide-(naked/BSA/OVA) | Details |
| GMP-CAN-NT-proBNP-Ab | Anti-Canine NT-proBNP (N-terminal prohormone of brain natriuretic peptide) antibody | Dog/Canine | Heart failure, cardiac disease | antibody(Fc/mFc) | Details |
| GMP-EQU-NT-proBNP-Ag | Equine NT-proBNP (N-terminal prohormone of brain natriuretic peptide) antigen | Equine/Horse | Cardiac disease | peptide-(naked/BSA/OVA) | Details |
| GMP-EQU-NT-proBNP-Ab | Anti-Equine NT-proBNP (N-terminal prohormone of brain natriuretic peptide) antibody | Equine/Horse | Cardiac disease | antibody(Fc/mFc) | Details |
| GMP-BOV-NT-proBNP-Ag | Bovine NT-proBNP (N-terminal prohormone of brain natriuretic peptide) antigen | Bovines/Cattle | Cardiac disease | peptide-(naked/BSA/OVA) | Details |
| GMP-BOV-NT-proBNP-Ab | Anti-Bovine NT-proBNP (N-terminal prohormone of brain natriuretic peptide) antibody | Bovines/Cattle | Cardiac disease | antibody(Fc/mFc) | Details |
| GMP-FEL-cTnI-Ag | Feline Cardiac troponin I (cTnI) antigen | Cat/Feline | Myocardial injury, heart disease | protein | Details |
| GMP-FEL-cTnI-Ab | Anti-Feline Cardiac troponin I (cTnI) antibody | Cat/Feline | Myocardial injury, heart disease | antibody(Fc/mFc) | Details |
| GMP-CAN-cTnI-Ag | Canine Cardiac troponin I (cTnI) antigen | Dog/Canine | Myocardial injury, heart disease | protein | Details |
| GMP-CAN-cTnI-Ab | Anti-Canine Cardiac troponin I (cTnI) antibody | Dog/Canine | Myocardial injury, heart disease | antibody(Fc/mFc) | Details |
| GMP-EQU-cTnI-Ag | Equine Cardiac troponin I (cTnI) antigen | Equine/Horse | Myocardial injury, heart disease | protein | Details |
| GMP-EQU-cTnI-Ab | Anti-Equine Cardiac troponin I (cTnI) antibody | Equine/Horse | Myocardial injury, heart disease | antibody(Fc/mFc) | Details |
| GMP-BOV-cTnI-Ag | Bovine Cardiac troponin I (cTnI) antigen | Bovines/Cattle | myocardial infarction | protein | Details |
| GMP-BOV-cTnI-Ab | Anti-Bovine Cardiac troponin I (cTnI) antibody | Bovines/Cattle | myocardial infarction | antibody(Fc/mFc) | Details |
| GMP-FEL-cTnT-Ag | Feline Cardiac troponin T (cTnT) antigen | Cat/Feline | hypertrophic cardiomyopathy, restrictive and dilated cardiomyopathy. | protein | Details |
| GMP-FEL-cTnT-Ab | Anti-Feline Cardiac troponin T (cTnT) antibody | Cat/Feline | hypertrophic cardiomyopathy, restrictive and dilated cardiomyopathy. | antibody(Fc/mFc) | Details |
| GMP-CAN-cTnT-Ag | Canine Cardiac troponin T (cTnT) antigen | Dog/Canine | hypertrophic cardiomyopathy, restrictive and dilated cardiomyopathy. | protein | Details |
| GMP-CAN-cTnT-Ab | Anti-Canine Cardiac troponin T (cTnT) antibody | Dog/Canine | hypertrophic cardiomyopathy, restrictive and dilated cardiomyopathy. | antibody(Fc/mFc) | Details |
| GMP-EQU-cTnT-Ag | Equine Cardiac troponin T (cTnT) antigen | Equine/Horse | hypertrophic cardiomyopathy, restrictive and dilated cardiomyopathy. | protein | Details |
| GMP-EQU-cTnT-Ab | Anti-Equine Cardiac troponin T (cTnT) antibody | Equine/Horse | hypertrophic cardiomyopathy, restrictive and dilated cardiomyopathy. | antibody(Fc/mFc) | Details |
| GMP-BOV-cTnT-Ag | Bovine Cardiac troponin T (cTnT) antigen | Bovines/Cattle | hypertrophic cardiomyopathy, restrictive and dilated cardiomyopathy. | protein | Details |
| GMP-BOV-cTnT-Ab | Anti-Bovine Cardiac troponin T (cTnT) antibody | Bovines/Cattle | hypertrophic cardiomyopathy, restrictive and dilated cardiomyopathy. | antibody(Fc/mFc) | Details |
| GMP-FEL-sST2-Ag | Feline sST2 antigen | Cat/Feline | chronic and acute heart failure | protein | Details |
| GMP-FEL-sST2-Ab | Anti-Feline sST2 antibody | Cat/Feline | chronic and acute heart failure | antibody(Fc/mFc) | Details |
| GMP-CAN-sST2-Ag | Canine sST2 antigen | Dog/Canine | chronic and acute heart failure | protein | Details |
| GMP-CAN-sST2-Ab | Anti-Canine sST2 antibody | Dog/Canine | chronic and acute heart failure | antibody(Fc/mFc) | Details |
| GMP-EQU-sST2-Ag | Equine sST2 antigen | Equine/Horse | chronic and acute heart failure | protein | Details |
| GMP-EQU-sST2-Ab | Anti-Equine sST2 antibody | Equine/Horse | chronic and acute heart failure | antibody(Fc/mFc) | Details |
| GMP-BOV-sST2-Ag | Bovine sST2 antigen | Bovines/Cattle | chronic and acute heart failure | protein | Details |
| GMP-BOV-sST2-Ab | Anti-Bovine sST2 antibody | Bovines/Cattle | chronic and acute heart failure | antibody(Fc/mFc) | Details |
| GMP-FEL-proBNP-Ag | Feline pro B-type natriuretic peptide (proBNP) antigen | Cat/Feline | intrinsic cardiac dysfunction |
peptide-(naked/BSA/OVA) | Details |
| GMP-FEL-proBNP-Ab | Anti-Feline pro B-type natriuretic peptide (proBNP) antibody | Cat/Feline | intrinsic cardiac dysfunction |
antibody(Fc/mFc) | Details |
| GMP-CAN-proBNP-Ag | Canine pro B-type natriuretic peptide (proBNP) antigen | Dog/Canine | intrinsic cardiac dysfunction |
peptide-(naked/BSA/OVA) | Details |
| GMP-CAN-proBNP-Ab | Anti-Canine pro B-type natriuretic peptide (proBNP) antibody | Dog/Canine | intrinsic cardiac dysfunction |
antibody(Fc/mFc) | Details |
| GMP-EQU-proBNP-Ag | Equine pro B-type natriuretic peptide (proBNP) antigen | Equine/Horse | intrinsic cardiac dysfunction |
peptide-(naked/BSA/OVA) | Details |
| GMP-EQU-proBNP-Ab | Anti-Equine pro B-type natriuretic peptide (proBNP) antibody | Equine/Horse | intrinsic cardiac dysfunction |
antibody(Fc/mFc) | Details |
| GMP-BOV-proBNP-Ag | Bovine pro B-type natriuretic peptide (proBNP) antigen | Bovines/Cattle | intrinsic cardiac dysfunction |
peptide-(naked/BSA/OVA) | Details |
| GMP-BOV-proBNP-Ab | Anti-Bovine pro B-type natriuretic peptide (proBNP) antibody | Bovines/Cattle | intrinsic cardiac dysfunction |
antibody(Fc/mFc) | Details |
| GMP-FEL-BNP-Ag | Feline B-type natriuretic peptide (BNP) antigen | Cat/Feline | Cardiovascular Disease | protein | Details |
| GMP-FEL-BNP-Ab | Anti-Feline B-type natriuretic peptide (BNP) antibody | Cat/Feline | Cardiovascular Disease | antibody(Fc/mFc) | Details |
| GMP-CAN-BNP-Ag | Canine B-type natriuretic peptide (BNP) antigen | Dog/Canine | Cardiovascular Disease | protein | Details |
| GMP-CAN-BNP-Ab | Anti-Canine B-type natriuretic peptide (BNP) antibody | Dog/Canine | Cardiovascular Disease | antibody(Fc/mFc) | Details |
| GMP-EQU-BNP-Ag | Equine B-type natriuretic peptide (BNP) antigen | Equine/Horse | Cardiovascular Disease | protein | Details |
| GMP-EQU-BNP-Ab | Anti-Equine B-type natriuretic peptide (BNP) antibody | Equine/Horse | Cardiovascular Disease | antibody(Fc/mFc) | Details |
| GMP-BOV-BNP-Ag | Bovine B-type natriuretic peptide (BNP) antigen | Bovines/Cattle | Cardiovascular Disease | protein | Details |
| GMP-BOV-BNP-Ab | Anti-Bovine B-type natriuretic peptide (BNP) antibody | Bovines/Cattle | Cardiovascular Disease | antibody(Fc/mFc) | Details |
| GMP-FEL-NT-proANP-Ag | Feline pro atrial natriuretic peptide (ANP) antigen | Cat/Feline | hypertension, cardiac hypertrophy and heart failure | protein | Details |
| GMP-FEL-NT-proANP-Ab | Anti-Feline pro atrial natriuretic peptide (ANP) antibody | Cat/Feline | hypertension, cardiac hypertrophy and heart failure | antibody(Fc/mFc) | Details |
| GMP-CAN-NT-proANP-Ag | Canine pro atrial natriuretic peptide (ANP) antigen | Dog/Canine | hypertension, cardiac hypertrophy and heart failure | protein | Details |
| GMP-CAN-NT-proANP-Ab | Anti-Canine pro atrial natriuretic peptide (ANP) antibody | Dog/Canine | hypertension, cardiac hypertrophy and heart failure | antibody(Fc/mFc) | Details |
| GMP-EQU-NT-proANP-Ag | Equine pro atrial natriuretic peptide (ANP) antigen | Equine/Horse | hypertension, cardiac hypertrophy and heart failure | protein | Details |
| GMP-EQU-NT-proANP-Ab | Anti-Equine pro atrial natriuretic peptide (ANP) antibody | Equine/Horse | hypertension, cardiac hypertrophy and heart failure | antibody(Fc/mFc) | Details |
| GMP-BOV-NT-proANP-Ag | Bovine pro atrial natriuretic peptide (ANP) antigen | Bovines/Cattle | hypertension, cardiac hypertrophy and heart failure | protein | Details |
| GMP-BOV-NT-proANP-Ab | Anti-Bovine pro atrial natriuretic peptide (ANP) antibody | Bovines/Cattle | hypertension, cardiac hypertrophy and heart failure | antibody(Fc/mFc) | Details |
| GMP-FEL-ANP-Ag | Feline atrial natriuretic peptide (ANP) antigen | Cat/Feline | hypertension, cardiac hypertrophy and heart failure | protein | Details |
| GMP-FEL-ANP-Ab | Anti-Feline atrial natriuretic peptide (ANP) antibody | Cat/Feline | hypertension, cardiac hypertrophy and heart failure | antibody(Fc/mFc) | Details |
| GMP-CAN-ANP-Ag | Canine atrial natriuretic peptide (ANP) antigen | Dog/Canine | hypertension, cardiac hypertrophy and heart failure | protein | Details |
| GMP-CAN-ANP-Ab | Anti-Canine atrial natriuretic peptide (ANP) antibody | Dog/Canine | hypertension, cardiac hypertrophy and heart failure | antibody(Fc/mFc) | Details |
| GMP-EQU-ANP-Ag | Equine atrial natriuretic peptide (ANP) antigen | Equine/Horse | hypertension, cardiac hypertrophy and heart failure | protein | Details |
| GMP-EQU-ANP-Ab | Anti-Equine atrial natriuretic peptide (ANP) antibody | Equine/Horse | hypertension, cardiac hypertrophy and heart failure | antibody(Fc/mFc) | Details |
| GMP-BOV-ANP-Ag | Bovine atrial natriuretic peptide (ANP) antigen | Bovines/Cattle | hypertension, cardiac hypertrophy and heart failure | protein | Details |
| GMP-BOV-ANP-Ab | Anti-Bovine atrial natriuretic peptide (ANP) antibody | Bovines/Cattle | hypertension, cardiac hypertrophy and heart failure | antibody(Fc/mFc) | Details |
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