INDKan Industry-Used AAV Vector System
AAV Vector Systems Kanamycin (Kan) resistance - Introduction
In the field of gene therapy, particularly in clinical research, the use of ampicillin-resistant plasmids is becoming less favored due to specific challenges, such as the risk of antibiotic resistance spreading to non-target bacteria. To address this, GeneMedi has made significant advancements in its AAV Helper Plasmid and AAV Rep-Cap Plasmids by replacing the ampicillin resistance marker with kanamycin (Kan) resistance.
This shift to kanamycin resistance is especially advantageous for industrial applications. Kanamycin is not only less prone to cross-resistance issues but also tends to be more stable under various industrial processing conditions. This stability is crucial for maintaining the integrity of gene therapy products during large-scale production. Moreover, the kanamycin resistance marker is better suited for regulatory approval processes, as it is generally considered safer and more environmentally benign compared to ampicillin.
GeneMedi's adoption of kanamycin-resistant vectors represents a forward-thinking approach, ensuring that their AAV plasmids are not only effective but also align with current best practices in biomanufacturing and safety standards. This transition is indicative of a broader industry trend towards safer, more sustainable genetic tools that minimize potential ecological impacts and enhance the overall feasibility of gene therapy products.
AAV Vector Systems Kanamycin (Kan) resistance - Product list
AAV Rep-cap plasmids of different serotypes
| Serotype | Voctor system description | Plasmid Name | Size | Products Information |
|---|---|---|---|---|
| AAV1 | AAV1 Vector System (Kan resistance) 1. AAV1 Rep-Cap Plasmid (Kan resistance), 2. AAV Helper Plasmid (Kan resistance), 3. pGMAAV-CMV-MCS-T2A-ZsGreen (Kan resistance) (P-AAVV-B01-Kan) |
AAV1 Rep-Cap Plasmid (Kan resistance) | ≥10mg | Details |
Choose from a wide range of AAV serotypes and variants tailored to your research needs:
| Common Serotypes | AAV1, AAV2, AAV4, AAV5, AAV6, AAV6.2, AAV6.2FF, AAV8, AAV9, AAV-Rh.10, AAV-DJ, AAV-Retro (Retrograde), AAV-PHP.B, AAV-PHP.eB, AAV-PHP.S, AAV-BR1, AAV-2i8, AAV-SIG, AAV-VEC. |
| AAV2 Variants | AAV2 variant (Y444F), AAV2 variant (Y272F, Y444F, Y500F, Y730F), AAV2 variant (Y444F, Y730F, Y500F, Y272F, Y704F, Y252F), AAV2 variant (AAV2.7m8). |
| AAV8 Variants | AAV8-1m, AAV8-2m, AAV8 variant (Y733F, Y447F, Y275). |
| MyoAAV Series | AAV-MyoAAV1A, AAV-MyoAAV2A, AAV-MyoAAV3A, AAV-MyoAAV4A, AAV-MyoAAV4E. |
| Other Specialized Variants | AAV-Anc80, AAV-rh74., AAV-LK03 |
AAV Expression Vector
Choose from Promoters: CMV, CAG, hSyn, CamKII, GFAP, cTNT, TBG, U6, EF1, EF1a
| Cat.No | Expression | Name of Vectors | Promoter | Reporter | Cre-dependent | TAG (unfusion) | Size | Products Information |
|---|---|---|---|---|---|---|---|---|
| P-AAVV-B01-Kan | overexpression | pGMAAV-CMV-MCS-T2A-ZsGreen (Kan resistance) | CMV | ZsGreen | No | null | ≥10mg | Details |
| P-AAVV-B02-Kan | overexpression | pGMAAV-CMV-MCS-3flag-P2A-Zsgreen (Kan resistance) | CMV | ZsGreen | No | 3FLAG(C-terminal) | ≥10mg | Details |
| P-AAVV-B03-Kan | overexpression | pGMAAV-CMV-MCS-3flag-EF1-ZsGreen (Kan resistance) | CMV | ZsGreen | No | 3FLAG(C-terminal) | ≥10mg | Details |
| P-AAVV-B04-Kan | overexpression | pGMAAV-CAG-MCS-T2A-ZsGreen (Kan resistance) | CAG | ZsGreen | No | null | ≥10mg | Details |
| P-AAVV-B05-Kan | overexpression | pGMAAV-CAG-MCS-3flag-T2A-ZsGreen (Kan resistance) | CAG | ZsGreen | No | 3FLAG(C-terminal) | ≥10mg | Details |
| P-AAVV-B06-Kan | overexpression | pGMAAV-CAG-MCS-T2A-mCherry (Kan resistance) | CAG | mCherry | No | null | ≥10mg | Details |
| P-AAVV-B07-Kan | overexpression | pGMAAV-CAG-DIO-MCS-T2A-ZsGreen (Kan resistance) | CAG | ZsGreen | Yes | null | ≥10mg | Details |
| P-AAVV-B08-Kan | overexpression | pGMAAV-CAG-DIO-MCS-T2A-mCherry (Kan resistance) | CAG | mCherry | Yes | null | ≥10mg | Details |
| P-AAVV-B09-Kan | overexpression | pGMAAV-CMV-DIO-MCS-T2A-ZsGreen (Kan resistance) | CMV | ZsGreen | Yes | null | ≥10mg | Details |
| P-AAVV-B10-Kan | overexpression | pGMAAV-hSyn-DIO-MCS-ZsGreen (Kan resistance) | hSyn | ZsGreen | Yes | null | ≥10mg | Details |
| P-AAVV-B11-Kan | overexpression | pGMAAV-hSyn-DIO-MCS-mCherry (Kan resistance) | hSyn | mCherry | Yes | null | ≥10mg | Details |
| P-AAVV-B12-Kan | overexpression | pGMAAV-hSyn-MCS-T2A-ZsGreen (Kan resistance) | hSyn | ZsGreen | No | null | ≥10mg | Details |
| P-AAVV-B13-Kan | overexpression | pGMAAV-hSyn-MCS-T2A-mCherry (Kan resistance) | hSyn | mCherry | No | null | ≥10mg | Details |
| P-AAVV-B14-Kan | overexpression | pGMAAV-CamKII-MCS-T2A-ZsGreen (Kan resistance) | CamKII | ZsGreen | No | null | ≥10mg | Details |
| P-AAVV-B15-Kan | overexpression | pGMAAV-CamKII-MCS-T2A-mCherry (Kan resistance) | CamKII | mCherry | No | null | ≥10mg | Details |
| P-AAVV-B16-Kan | overexpression | pGMAAV-GFAP-MCS-T2A-EGFP (Kan resistance) | GFAP | EGFP | No | null | ≥10mg | Details |
| P-AAVV-B17-Kan | overexpression | pGMAAV-cTNT-MCS-ZsGreen (Kan resistance) | cTNT | ZsGreen | No | null | ≥10mg | Details |
| P-AAVV-B18-Kan | overexpression | pGMAAV-GFAP-EGFP (Kan resistance) | GFAP | EGFP | No | null | ≥10mg | Details |
| P-AAVV-B19-Kan | overexpression | pGMAAV-TBG-MCS-P2A-ZsGreen (Kan resistance) | TBG | ZsGreen | No | null | ≥10mg | Details |
| P-AAVV-B20-Kan | shRNA for RNAi | pGMAAV-U6-MCS-CMV-mCherry (Kan or istance) | U6 | mCherry | No | null | ≥10mg | Details |
| P-AAVV-B21-Kan | shRNA for RNAi | pGMAAV-U6-MCS-CMV-EGFP (Kan or istance) | U6 | EGFP | No | null | ≥10mg | Details |
| P-AAVV-B22-Kan | shRNA for RNAi | pGMAAV-U6-MCS-WPRE (Kan or istance) | U6 | null | No | null | ≥10mg | Details |
| P-AAVV-B23-Kan | Cas9/gRNAfor Konckout | pGMAAV-CMV-Sacas9-U6-gRNA (Kan NAfistance) | CMV | null | No | null | ≥10mg | Details |
| P-AAVV-B24-Kan | gRNAfor Konckout | pscAAV-U6-BB-chemeric-sgRNA (Kan Koistance) | U6 | null | No | null | ≥10mg | Details |
| P-AAVV-B25-Kan | overexpression | pGMAAV-CMV-MCS-EF1-mCherry (Kan resistance) | CMV | mCherry | No | null | ≥10mg | Details |
| P-AAVV-B26-Kan | overexpression | pGMAAV-CMV-MCS-T2A-mCherry (Kan resistance) | CMV | mCherry | No | null | ≥10mg | Details |
| P-AAVV-B27-Kan | overexpression | pGMAAV-CMV-DIO-MCS (Kan resistance) | CMV | null | Yes | null | ≥10mg | Details |
| P-AAVV-B28-Kan | overexpression | pGMAAV-CMV-DIO-MCS-T2Am-ZsGreen (Kan resistance) | CMV | ZsGreen | Yes | null | ≥10mg | Details |
| P-AAVV-B29-Kan | overexpression | pGMAAV-CMV-DIO-ZsGreen-MCS (Kan resistance) | CMV | ZsGreen | Yes | null | ≥10mg | Details |
| P-AAVV-B30-Kan | overexpression | pGMAAV-CAG-DIO-MCS (Kan resistance) | CAG | null | Yes | null | ≥10mg | Details |
| P-AAVV-B31-Kan | overexpression | pGMAAV-hSyn-MCS-T2Am-mCherry (Kan resistance) | hSyn | mCherry | No | null | ≥10mg | Details |
| P-AAVV-B32-Kan | overexpression | pGMAAV-hSyn-ZsGreen (Kan resistance) | hSyn | ZsGreen | No | null | ≥10mg | Details |
| P-AAVV-B33-Kan | overexpression | pGMAAV-cTNT-MCS-P2A-EGFP (Kan resistance) | cTNT | EGFP | No | null | ≥10mg | Details |
| P-AAVV-B34-Kan | overexpression | pGMAAV-TBG-MCS-P2A-mCherry (Kan resistance) | TBG | mCherry | No | null | ≥10mg | Details |
| P-AAVV-B35-Kan | shRNA for RNAi | pGMAAV-U6-MCS-CMV-LUCi (Kan or istance) | U6 | null | No | null | ≥10mg | Details |
| P-AAVV-B36-Kan | shRNA for RNAi | pGMAAV-U6-MCS-CMV-ZsGreen (Kan or istance) | U6 | ZsGreen | No | null | ≥10mg | Details |
| P-AAVV-B37-Kan | overexpression | pGMAAV-CTNT-MCS-CMV-ZsGreen (Kan resistance) | cTNT | ZsGreen | No | null | ≥10mg | Details |
| P-AAVV-B38-Kan | for Konckout | pscAAV-U6-BB-sgRNA-CMV-mCherry-new (Kan ckoistance) | U6 | mCherry | No | null | ≥10mg | Details |
| P-AAVV-B39-Kan | for Konckout | pscAAV-U6-BB-sgRNA-CMV-EGFP-new (Kan ckoistance) | U6 | EGFP | No | null | ≥10mg | Details |
| P-AAVV-B40-Kan | overexpressioncre | pGMAAV-cre-ZsGreen (Kan resistance) | CMV | ZsGreen | No | 3FLAG(C-terminal) | ≥10mg | Details |
| P-AAVV-B41-Kan | overexpressioncre | pGMAAV-hSyn-cre (Kan resistance) | hSyn | null | No | null | ≥10mg | Details |
| P-AAVV-B42-Kan | overexpression | pGMAAV-TBG-MCS-P2A-mCherry (Kan resistance) | TBG | mCherry | No | null | ≥10mg | Details |
| P-AAVV-B43-Kan | overexpression | pGMAAV-GFAP-MCS-P2A-mCherry (Kan resistance) | GFAP | mCherry | No | null | ≥10mg | Details |
| P-AAVV-B44-Kan | overexpression | pGMAAV-CAG-DIO-T2Am-EGFP (Kan resistance) | CAG | EGFP | Yes | null | ≥10mg | Details |
| P-AAVV-B45-Kan | overexpression | pGMAAV-CMV-DIO-EGFP (Kan resistance) | CMV | EGFP | Yes | null | ≥10mg | Details |
| P-AAVV-B46-Kan | shRNA for RNAi | pGMAAV-U6-MCS-CMV-luc(△EGFP) (Kan or istance) | U6 | null | No | null | ≥10mg | Details |
| P-AAVV-B47-Kan | overexpression | pGMAAV-CMV-MCS-EF1-EGFP(△WPRE) (Kan resistance) | CMV | EGFP | No | null | ≥10mg | Details |
| P-AAVV-B48-Kan | overexpression | pGMAAV-EF1-MCS-CMV-EGFP (Kan resistance) | EF1 | EGFP | No | null | ≥10mg | Details |
| P-AAVV-B49-Kan | overexpression | pGMAAV-hCAG-MCS-P2A-ZsGreen (Kan resistance) | CAG | ZsGreen | No | null | ≥10mg | Details |
| P-AAVV-B50-Kan | overexpression | pGMAAV-CMV-MCS-P2A-ZsGreen (Kan resistance) | CMV | ZsGreen | No | null | ≥10mg | Details |
| P-AAVV-B51-Kan | overexpression | pGMAAV-CMV-MCS-P2A-luc(△EGFP) (Kan resistance) | CMV | luc | No | null | ≥10mg | Details |
| P-AAVV-B52-Kan | overexpression | pGMAAV-hSyn-MCS-T2Am-ZsGreen (Kan resistance) | hSyn | ZsGreen | No | null | ≥10mg | Details |
| P-AAVV-B53-Kan | overexpression | pGMAAV-TNT-3flag-P2A-EGFP (Kan resistance) | cTNT | EGFP | No | 3FLAG(C-terminal) | ≥10mg | Details |
| P-AAVV-B55-Kan | overexpression | pGMAAV-pGMAAV-cTNT-MCS (Kan resistance) | cTNT | null | No | null | ≥10mg | Details |
| P-AAVV-B56-Kan | overexpression | pGMAAV-CMV-MCS-T2A-mcherry (Kan resistance) | CMV | mCherry | No | null | ≥10mg | Details |
| P-AAVV-B57-Kan | overexpression | pGMAAV-cTNT-MCS-3flag-P2A-EGFP (Kan resistance) | cTNT | EGFP | No | 3FLAG(C-terminal) | ≥10mg | Details |
| P-AAVV-B58-Kan | overexpression | pGMAAV-hSyn-cre (Kan resistance) | hSyn | null | No | null | ≥10mg | Details |
| P-AAVV-B59-Kan | overexpression | pscAAV-U6-BB-sgRNA-PGK-EGFP (Kan resistance) | U6 | EGFP | No | null | ≥10mg | Details |
| P-AAVV-B60-Kan | overexpression | pGMAAV-CMV-DIO-tdtomato (Kan resistance) | CMV | tdtomato | Yes | null | ≥10mg | Details |
| P-AAVV-B61-Kan | overexpression | pGMAAV-CAG-DIO-T2Am-tdtomato (Kan resistance) | CAG | tdtomato | Yes | null | ≥10mg | Details |
| P-AAVV-B62-Kan | overexpression | pGMAAV-EF1a-DIO-FLPo-WPRE-hGHpA (Kan resistance) | EF1a | null | Yes | null | ≥10mg | Details |
| P-AAVV-B63-Kan | overexpression | pGMAAV-CAG-MCS (NO WPRE) (Kan resistance) | CAG | null | No | null | ≥10mg | Details |
| P-AAVV-B64-Kan | overexpression | pGMAAV-CAG-MCS-T2A-ZsGreen (NO WPRE) (Kan resistance) | CAG | ZsGreen | No | null | ≥10mg | Details |
| P-AAVV-B65-Kan | overexpression | pGMAAV-CMV-MCS (NO WPRE) (Kan resistance) | CMV | null | No | null | ≥10mg | Details |
| P-AAVV-B66-Kan | overexpression | pGMAAV-CMV-MCS-T2A-ZsGreen (NO WPRE) (Kan resistance) | CMV | ZsGreen | No | null | ≥10mg | Details |
| P-AAVV-B67-Kan | overexpression | pGMAAV-TBG-MCS-CMV-ZsGreen (Kan resistance) | TBG | ZsGreen | No | null | ≥10mg | Details |
| P-AAVV-B68-Kan | overexpression | pGMAAV-TBG-MCS-NULL (Kan resistance) | TBG | null | No | null | ≥10mg | Details |
| P-AAVV-B69-Kan | overexpression | pGMAAV-CMV-circ-EF1-ZsGreen (Kan resistance) | CMV | ZsGreen | No | null | ≥10mg | Details |
| P-AAVV-B70-Kan | overexpression | pGMAAV-CMV-MCS-T2Am-ZsGreen (Kan resistance) | CMV | ZsGreen | No | null | ≥10mg | Details |
| P-AAVV-B71-Kan | overexpression | pGMAAV-CMV-MCS-3flag (Kan resistance) | CMV | null | No | 3FLAG(C-terminal) | ≥10mg | Details |
| P-AAVV-B72-Kan | overexpression | pGMAAV-GFAP-MCS-NULL (Kan resistance) | GFAP | null | No | null | ≥10mg | Details |
| P-AAVV-B73-Kan | overexpression | pGMAAV-cTNT-mCherry (Kan resistance) | cTNT | mCherry | No | null | ≥10mg | Details |
| P-AAVV-B74-Kan | shRNA for RNAi | pGMAAV-U6-MCS-CMV-eRFP (Kan or istance) | U6 | eRFP | No | null | ≥10mg | Details |
| P-AAVV-B75-Kan | overexpression | pGMAAV-CMV-MCS-EF1-eRFP (Kan resistance) | CMV | eRFP | No | null | ≥10mg | Details |
| P-AAVV-B76-Kan | overexpression | pGMAAV-CMV-MCS-3flag-T2A-eRFP (Kan resistance) | CMV | eRFP | No | 3FLAG(C-terminal) | ≥10mg | Details |
| P-AAVV-B77-Kan | overexpression | pGMAAV-hsyn-DIO-MCS (Kan resistance) | hsyn | null | Yes | null | ≥10mg | Details |
| P-AAVV-B78-Kan | shRNA for RNAi | pGMAAV-U6-DIO-CMV-mcherry (Kan or istance) | U6 | mcherry | Yes | null | ≥10mg | Details |
| P-AAVV-B79-Kan | overexpression | pGMAAV-CMV-T2A-ZsGreen-W3-BGH-pa (Kan resistance) | CMV | ZsGreen | No | null | ≥10mg | Details |
| P-AAVV-B80-Kan | overexpression | pGMAAV-cTNT-LUCi (Kan resistance) | cTNT | LUCi | No | null | ≥10mg | Details |
| P-AAVV-B81-Kan | shRNA for RNAi | pGMAAV-U6-DIO-CMV-EGFP (Kan or istance) | U6 | EGFP | Yes | null | ≥10mg | Details |
| P-AAVV-B82-Kan | overexpression | pGMAAV-CMV-3flag-EF1-ZsGreen-w3-bgh pa (Kan resistance) | CMV | ZsGreen | No | 3FLAG(C-terminal) | ≥10mg | Details |
| P-AAVV-B83-Kan | overexpression | pGMAAV-CMV-MCS-EF1-luc (Kan resistance) | CMV | luc | No | null | ≥10mg | Details |
| P-AAVV-B84-Kan | overexpression | pGMAAV-TBG-P2A-LUCi (Kan resistance) | TBG | LUCi | No | null | ≥10mg | Details |
| P-AAVV-B85-Kan | overexpression | pGMAAV-CMV-MCS-3flag-IRES-hrGFP (Kan resistance) | CMV | hrGFP | No | 3FLAG(C-terminal) | ≥10mg | Details |
| P-AAVV-B86-Kan | overexpression | pGMAAV-GFAP-MCS-T2A-ZsGreen (Kan resistance) | GFAP | ZsGreen | No | null | ≥10mg | Details |
AAV Helper Vector
| Cat.No | Description | Map and sequence | Size | Products Information |
|---|---|---|---|---|
| P-HP01-Kan | AAV Helper Plasmid (Kan resistance) | 
 Download the detail information of AAV Helper Plasmid here and read in Snapgene Software. |
≥10mg | Details |
AAV Vector Systems Kanamycin (Kan) resistance - Stability data
GeneMedi's AAV vector systems with kanamycin resistance exhibit stable plasmid amplification yields, high purity, and enhanced AAV potency.
1. Consistent Batch-to-Batch Yield Stability
As shown in Figure A, GeneMedi's plasmid amplification service ensures that the yield of AAV plasmids with kanamycin resistance remains stable across multiple batches, regardless of the plasmid copy number (low, medium, or high). This consistent yield is crucial for maintaining the quality and efficiency of gene therapy production processes.
2. High Purity with ≥90% Supercoiled Content (PAGE Analysis)
Figure B demonstrates the high purity of AAV plasmids with kanamycin resistance, with supercoiled content exceeding 90%. This high purity ensures that the plasmids are suitable for demanding applications.
3. Enhanced AAV Potency for Superior Gene Delivery
Figure C illustrates the excellent infectivity of AAV2, AAV8, and AAV9 viruses, which were packaged using GeneMedi's kanamycin-resistant AAV plasmids. The strong green fluorescence in 293 cells highlights the high potency of the AAV vectors, making them highly effective for gene therapy applications.
Fig. GeneMedi's AAV vector systems with kanamycin resistance exhibit stable plasmid yields, high purity, and enhanced AAV potency.
A. Plasmid yield stability across different copy number plasmids (low, medium, high) with kanamycin resistance.
B. PAGE gel showing plasmid purity, with ≥90% supercoiled content.
CC. Green fluorescence image showing successful infection of 293 cells with AAV2, AAV8, and AAV9 viruses prepared using GeneMedi's kanamycin-resistant AAV vector system.
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